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The map label for this gene is dnaA

Identifier: 189025237

GI number: 189025237

Start: 4

End: 1398

Strand: Direct

Name: dnaA

Synonym: TPASS_0001

Alternate gene names: 189025237

Gene position: 4-1398 (Clockwise)

Preceding gene: NA

Following gene: 189025238

Centisome position: 0.0

GC content: 40.72

Gene sequence:

>1395_bases
ATGGACGCAGTAGGGTATGAAGTATTCTGGAACGAGACACTCAGCCAGATACGGAGTGAATCGACCGAAGCAGAATTTAA
CATGTGGTTTGCTCATTTGTTCTTTATCGCATCTTTTGAAAACGCTATCGAAATAGCAGTACCTTCAGACTTTTTCCGAA
TACAGTTTAGCCAAAAATATCAAGAAAAGCTTGAGCGCAAGTTCCTCGAACTTTCTGGACACCCCATTAAACTTTTGTTT
GCCGTTAAAAAAGGCACCCCTCATGGAAATACTGCTCCCCCCAAACACGTGCATACCTACCTGGAGAAAAACTCTCCTGC
AGAGGTTCCTTCCAAAAAGAGCTTTCACCCCGACCTGAACAGAGACTATACCTTCGAGAACTTTGTATCCGGAGAAGAAA
CCAAATTCAGCCATAGCGCTGCTATCTCCGTATCAAAAAACCCAGGCACTTCCTACAATCCGTTACTTATCTACGGTGGA
GTGGGACTAGGAAAAACCCACCTTATGCAGGCTATTGGACACGAGATCTACAAGACAACAGACCTGAACGTCATATACGT
CACTGCGGAGAATTTTGGAAATGAATTCATTTCCACATTACTCAATAAAAAGACCCAGGATTTTAAAAAAAAATACCGCT
ACACCGCGGATGTACTTCTTATAGATGACATTCATTTTTTTGAAAACAAAGACGGATTACAAGAAGAGCTTTTCTATACG
TTCAACGAACTTTTCGAGAAAAAAAAACAAATTATCTTTACCTGCGACAGGCCTGTACAAGAATTGAAAAATCTCTCTTC
TCGCTTACGCTCGAGGTGCTCCCGAGGGCTTAGCACTGATCTGAATATGCCATGTTTTGAAACGCGCTGTGCTATCTTGA
TTAAAAAAATACAAAACTATAACAGCACCTATCCTCACAAAGCCATCCACATTTCAGACGATGTTGTCCGACTTGTTTCT
GAAAACATTTCTTCAAATATCAGGGATCTTGAGGGGGCATTAACAAAAATTATCGCTTTCATTGAAGTGTCGGGATCCAT
CACGATAGATATCGTTCCCTCTCTCCTAAAAGAGTTCTTCCTCTCTGCAAGGCCAAAACACATCACAGTAGAAACTATTC
TTCATGTAGTTGCAGATCACTTTAACATTTCGTATTCAGATCTAAAGGGTAAGAAACGCAATAAAAGCGTTGTTTATCCT
CGGCAAATCGCTATGTTTCTCTCAAAGGAACTGACAGAGCTCTCCACTACTGAACTTGGTATCGAATTTGGTGGCAGAGA
TCATTCAACCGTCATTTACGGATGTCAAAAAATAGAAGGAGAAATTCTCACTAATCCTTCGTTACAGGCAAATCTTGATT
TGCTGAAAAGTAAAGTTCAAGATTCAATCCGCTAG

Upstream 100 bases:

>100_bases
ACTTCTCTTGAATAACAAGATAATCTACACCCACAAAGCAACGCGCGTATAACCTCTTCGTTTATTGCAGGCAAAATACA
ATAGGTTGTATCATAGGTAG

Downstream 100 bases:

>100_bases
GGCGTAGACACTGAATTCGATGGGGATAAGTGGTGGATAAAAGAATATAAATTAGTCATTACACTTTACTCACGAATATC
CCCCTTTTTTTAGAGAAAAA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDAVGYEVFWNETLSQIRSESTEAEFNMWFAHLFFIASFENAIEIAVPSDFFRIQFSQKYQEKLERKFLELSGHPIKLLF
AVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLNRDYTFENFVSGEETKFSHSAAISVSKNPGTSYNPLLIYGG
VGLGKTHLMQAIGHEIYKTTDLNVIYVTAENFGNEFISTLLNKKTQDFKKKYRYTADVLLIDDIHFFENKDGLQEELFYT
FNELFEKKKQIIFTCDRPVQELKNLSSRLRSRCSRGLSTDLNMPCFETRCAILIKKIQNYNSTYPHKAIHISDDVVRLVS
ENISSNIRDLEGALTKIIAFIEVSGSITIDIVPSLLKEFFLSARPKHITVETILHVVADHFNISYSDLKGKKRNKSVVYP
RQIAMFLSKELTELSTTELGIEFGGRDHSTVIYGCQKIEGEILTNPSLQANLDLLKSKVQDSIR

Sequences:

>Translated_464_residues
MDAVGYEVFWNETLSQIRSESTEAEFNMWFAHLFFIASFENAIEIAVPSDFFRIQFSQKYQEKLERKFLELSGHPIKLLF
AVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLNRDYTFENFVSGEETKFSHSAAISVSKNPGTSYNPLLIYGG
VGLGKTHLMQAIGHEIYKTTDLNVIYVTAENFGNEFISTLLNKKTQDFKKKYRYTADVLLIDDIHFFENKDGLQEELFYT
FNELFEKKKQIIFTCDRPVQELKNLSSRLRSRCSRGLSTDLNMPCFETRCAILIKKIQNYNSTYPHKAIHISDDVVRLVS
ENISSNIRDLEGALTKIIAFIEVSGSITIDIVPSLLKEFFLSARPKHITVETILHVVADHFNISYSDLKGKKRNKSVVYP
RQIAMFLSKELTELSTTELGIEFGGRDHSTVIYGCQKIEGEILTNPSLQANLDLLKSKVQDSIR
>Mature_464_residues
MDAVGYEVFWNETLSQIRSESTEAEFNMWFAHLFFIASFENAIEIAVPSDFFRIQFSQKYQEKLERKFLELSGHPIKLLF
AVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLNRDYTFENFVSGEETKFSHSAAISVSKNPGTSYNPLLIYGG
VGLGKTHLMQAIGHEIYKTTDLNVIYVTAENFGNEFISTLLNKKTQDFKKKYRYTADVLLIDDIHFFENKDGLQEELFYT
FNELFEKKKQIIFTCDRPVQELKNLSSRLRSRCSRGLSTDLNMPCFETRCAILIKKIQNYNSTYPHKAIHISDDVVRLVS
ENISSNIRDLEGALTKIIAFIEVSGSITIDIVPSLLKEFFLSARPKHITVETILHVVADHFNISYSDLKGKKRNKSVVYP
RQIAMFLSKELTELSTTELGIEFGGRDHSTVIYGCQKIEGEILTNPSLQANLDLLKSKVQDSIR

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=451, Percent_Identity=36.8070953436807, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_TREPA (O83047)

Other databases:

- EMBL:   AE000520
- PIR:   H71377
- RefSeq:   NP_218442.1
- ProteinModelPortal:   O83047
- IntAct:   O83047
- GeneID:   2611816
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0001
- NMPDR:   fig|243276.1.peg.1
- TIGR:   TP_0001
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- ProtClustDB:   PRK00149
- BioCyc:   TPAL243276:TP_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52966; Mature: 52966

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVGYEVFWNETLSQIRSESTEAEFNMWFAHLFFIASFENAIEIAVPSDFFRIQFSQKY
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEHHHH
QEKLERKFLELSGHPIKLLFAVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLN
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
RDYTFENFVSGEETKFSHSAAISVSKNPGTSYNPLLIYGGVGLGKTHLMQAIGHEIYKTT
CCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEC
DLNVIYVTAENFGNEFISTLLNKKTQDFKKKYRYTADVLLIDDIHFFENKDGLQEELFYT
CCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCHHHCCCCCHHHHHHHH
FNELFEKKKQIIFTCDRPVQELKNLSSRLRSRCSRGLSTDLNMPCFETRCAILIKKIQNY
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
NSTYPHKAIHISDDVVRLVSENISSNIRDLEGALTKIIAFIEVSGSITIDIVPSLLKEFF
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHH
LSARPKHITVETILHVVADHFNISYSDLKGKKRNKSVVYPRQIAMFLSKELTELSTTELG
HCCCCCEEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IEFGGRDHSTVIYGCQKIEGEILTNPSLQANLDLLKSKVQDSIR
EEECCCCCCEEEEEEHHCCCEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAVGYEVFWNETLSQIRSESTEAEFNMWFAHLFFIASFENAIEIAVPSDFFRIQFSQKY
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEHHHH
QEKLERKFLELSGHPIKLLFAVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLN
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
RDYTFENFVSGEETKFSHSAAISVSKNPGTSYNPLLIYGGVGLGKTHLMQAIGHEIYKTT
CCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEC
DLNVIYVTAENFGNEFISTLLNKKTQDFKKKYRYTADVLLIDDIHFFENKDGLQEELFYT
CCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCHHHCCCCCHHHHHHHH
FNELFEKKKQIIFTCDRPVQELKNLSSRLRSRCSRGLSTDLNMPCFETRCAILIKKIQNY
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
NSTYPHKAIHISDDVVRLVSENISSNIRDLEGALTKIIAFIEVSGSITIDIVPSLLKEFF
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHH
LSARPKHITVETILHVVADHFNISYSDLKGKKRNKSVVYPRQIAMFLSKELTELSTTELG
HCCCCCEEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IEFGGRDHSTVIYGCQKIEGEILTNPSLQANLDLLKSKVQDSIR
EEECCCCCCEEEEEEHHCCCEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876