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The map label for this gene is pyrB

Identifier: 188587358

GI number: 188587358

Start: 2985657

End: 2986598

Strand: Reverse

Name: pyrB

Synonym: Nther_2765

Alternate gene names: 188587358

Gene position: 2986598-2985657 (Counterclockwise)

Preceding gene: 188587359

Following gene: 188587357

Centisome position: 94.35

GC content: 39.28

Gene sequence:

>942_bases
TTGGGTCTACAGCGAAAAAGTCTACTGGGATTAAAAGATATGGAAAAGTCAGAAATAGAATCTATTCTTGATTCAACGGA
AAGTATGAAAGAGATAGTACAGAGAAAGAATAAAAAACTTCCAACATTAAAAGGCTTTTCCATGGTGAATTTATTTTATG
AACCTAGCACCAGGACCAGATCATCCTTTGAAATGGCGGGTAAGTACTTAGGTGCTGATACGACAAATATGAGTGCTTCT
GCTAGTAGTGTTGCTAAAGGAGAAAACCTAATTGATACAGGGAAGACGTTGGAAGCCATGGGAATAAACTTAATTGTGAT
GCGACATCCACTGGCGGGTGCTCCCCGTTTACTGGCAAATAATTTATCTTGTTCAATAATTAATGCCGGCGATGGTTATC
ATGAACATCCCACTCAGGCGCTTTTGGATATGTTTACAATTAAAGAATATAAAGGCTCTCTCAAAGGATTAAAGGTAACT
ATAATTGGTGATATTTATCACAGTCGAGTTGCCAGGTCTAATATCTGGGGACTTACTAAAATGGGTGCAGATGTTAGACT
TGCAGGCCCTCCAACTTTGCTTGATCAACGAGTGTTTACAGAGATGGGGGCTAGTTGTTATTACAGGGTAGAAGATGCTT
TGGCTGATGCCGATGTTGTGATGGCACTTCGAATCCAAAGAGAAAGACAGGGAAAAACACTGTTACCAAGTTTGAGAGAA
TATGCCCGATTATACGGGATTAATCAAGAAAGATTTAAACTTGCTAAAAAGGATGCCTTGCTACTTCATCCGGGACCTGT
TAATCGTGGTATTGAATTGACTTCTGAACTAATGAACAGCGAAAAATCAGTTATTGAAGAACAGGTGACTAACGGAGTTG
CTATCCGAATGGCATTGCTTTATCACTTATCTGGAGGTGAAGATAATGGGGATATTGCTTAA

Upstream 100 bases:

>100_bases
AAAATGAAGCATAGGAAGTTTTTTTATGGTCAATTTTACATCTGAAGAGTATTGCTTGTTGATATTTATTGACAATTAAC
TTATGATAGGGGGTTAAAAA

Downstream 100 bases:

>100_bases
GGGAGGTATTATTTATACAGGAGAACCCGTTAGTGATGGAACACAAATGGATATTTTAATAGAAGATGGTATGATTAAGG
ATCAACAAAGGAATATTAAT

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MGLQRKSLLGLKDMEKSEIESILDSTESMKEIVQRKNKKLPTLKGFSMVNLFYEPSTRTRSSFEMAGKYLGADTTNMSAS
ASSVAKGENLIDTGKTLEAMGINLIVMRHPLAGAPRLLANNLSCSIINAGDGYHEHPTQALLDMFTIKEYKGSLKGLKVT
IIGDIYHSRVARSNIWGLTKMGADVRLAGPPTLLDQRVFTEMGASCYYRVEDALADADVVMALRIQRERQGKTLLPSLRE
YARLYGINQERFKLAKKDALLLHPGPVNRGIELTSELMNSEKSVIEEQVTNGVAIRMALLYHLSGGEDNGDIA

Sequences:

>Translated_313_residues
MGLQRKSLLGLKDMEKSEIESILDSTESMKEIVQRKNKKLPTLKGFSMVNLFYEPSTRTRSSFEMAGKYLGADTTNMSAS
ASSVAKGENLIDTGKTLEAMGINLIVMRHPLAGAPRLLANNLSCSIINAGDGYHEHPTQALLDMFTIKEYKGSLKGLKVT
IIGDIYHSRVARSNIWGLTKMGADVRLAGPPTLLDQRVFTEMGASCYYRVEDALADADVVMALRIQRERQGKTLLPSLRE
YARLYGINQERFKLAKKDALLLHPGPVNRGIELTSELMNSEKSVIEEQVTNGVAIRMALLYHLSGGEDNGDIA
>Mature_312_residues
GLQRKSLLGLKDMEKSEIESILDSTESMKEIVQRKNKKLPTLKGFSMVNLFYEPSTRTRSSFEMAGKYLGADTTNMSASA
SSVAKGENLIDTGKTLEAMGINLIVMRHPLAGAPRLLANNLSCSIINAGDGYHEHPTQALLDMFTIKEYKGSLKGLKVTI
IGDIYHSRVARSNIWGLTKMGADVRLAGPPTLLDQRVFTEMGASCYYRVEDALADADVVMALRIQRERQGKTLLPSLREY
ARLYGINQERFKLAKKDALLLHPGPVNRGIELTSELMNSEKSVIEEQVTNGVAIRMALLYHLSGGEDNGDIA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=35.9223300970874, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=27.1565495207668, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=33.0128205128205, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=31.8181818181818, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=33.2258064516129, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24642586, Length=323, Percent_Identity=33.4365325077399, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_NATTJ (B2A2U7)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918903.1
- ProteinModelPortal:   B2A2U7
- SMR:   B2A2U7
- GeneID:   6315750
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2765
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34524; Mature: 34393

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQRKSLLGLKDMEKSEIESILDSTESMKEIVQRKNKKLPTLKGFSMVNLFYEPSTRTR
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCH
SSFEMAGKYLGADTTNMSASASSVAKGENLIDTGKTLEAMGINLIVMRHPLAGAPRLLAN
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHCCCHHHHCCCEEEEEECCCCCCCHHHHC
NLSCSIINAGDGYHEHPTQALLDMFTIKEYKGSLKGLKVTIIGDIYHSRVARSNIWGLTK
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCHHH
MGADVRLAGPPTLLDQRVFTEMGASCYYRVEDALADADVVMALRIQRERQGKTLLPSLRE
CCCCEEECCCCHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
YARLYGINQERFKLAKKDALLLHPGPVNRGIELTSELMNSEKSVIEEQVTNGVAIRMALL
HHHHHCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
YHLSGGEDNGDIA
HHHCCCCCCCCCC
>Mature Secondary Structure 
GLQRKSLLGLKDMEKSEIESILDSTESMKEIVQRKNKKLPTLKGFSMVNLFYEPSTRTR
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCH
SSFEMAGKYLGADTTNMSASASSVAKGENLIDTGKTLEAMGINLIVMRHPLAGAPRLLAN
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHCCCHHHHCCCEEEEEECCCCCCCHHHHC
NLSCSIINAGDGYHEHPTQALLDMFTIKEYKGSLKGLKVTIIGDIYHSRVARSNIWGLTK
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCHHH
MGADVRLAGPPTLLDQRVFTEMGASCYYRVEDALADADVVMALRIQRERQGKTLLPSLRE
CCCCEEECCCCHHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
YARLYGINQERFKLAKKDALLLHPGPVNRGIELTSELMNSEKSVIEEQVTNGVAIRMALL
HHHHHCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
YHLSGGEDNGDIA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA