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The map label for this gene is fumB [H]

Identifier: 188587005

GI number: 188587005

Start: 2561287

End: 2561847

Strand: Reverse

Name: fumB [H]

Synonym: Nther_2397

Alternate gene names: 188587005

Gene position: 2561847-2561287 (Counterclockwise)

Preceding gene: 188587006

Following gene: 188587004

Centisome position: 80.93

GC content: 45.63

Gene sequence:

>561_bases
ATGCGTGATCAGTTTTATAAAAAACTTTCAGCTCCTCTTGCTGAGGAAGAGGTTCAAGAACTCAAGGTGGGAGATCAGGT
GCTGCTTTCAGGGTCAGTCTTGACTGCCAGGGATGCATCCCACAAGAGGTTAATAGAGCTGAAGAAAGCCGGGATTGAAC
TACCAGTATCTTTAGAAGGTGAAATTATTTATTATGTGGGTCCCACTCCTGCCAAACCGGGGCATGTCATAGGTTCGGCT
GGTCCAACTACCAGCAGCAGGATGGATAGATATACTACCCAAATGTTGGAACTAGGAGCCAAAGCATTCATAGGCAAGGG
TTATCGCTCTCAAACAGTTAAAAATGCGCTGCAAAAACACAAAGGGGTATACTTAGCTGCCGTCGGTGGGGCGGGAGCAC
TTATAAAGGAAAGTATAGTGGACTATCAAGTGATAGCCTTCGAAGACCTGGGGCCTGAGGCTATCAGGCGTTTAGAAGTT
AAAAATTTCCCGGTATTAGTTGCTAATGATATCTATGGCAATGACCTGTACCAACAGGGCAAGAATAGGTATGCCAAGTA
G

Upstream 100 bases:

>100_bases
TACATTGCGTTCAACAAAACTAAATAGAGAAGAGGAGAGATTAAGTGGATTAGCAGACTAAAAAAGATTGAAATTTCACA
ATTGGGAGGAAAATAAAATA

Downstream 100 bases:

>100_bases
CATGGTTTCATATTTATCACGATAGTGGGAGGTTTGGGATATGAAATTATATGAATATATGGCTAAGAAATTGATGAATC
AATGTGGTATTTCCGTACCC

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MRDQFYKKLSAPLAEEEVQELKVGDQVLLSGSVLTARDASHKRLIELKKAGIELPVSLEGEIIYYVGPTPAKPGHVIGSA
GPTTSSRMDRYTTQMLELGAKAFIGKGYRSQTVKNALQKHKGVYLAAVGGAGALIKESIVDYQVIAFEDLGPEAIRRLEV
KNFPVLVANDIYGNDLYQQGKNRYAK

Sequences:

>Translated_186_residues
MRDQFYKKLSAPLAEEEVQELKVGDQVLLSGSVLTARDASHKRLIELKKAGIELPVSLEGEIIYYVGPTPAKPGHVIGSA
GPTTSSRMDRYTTQMLELGAKAFIGKGYRSQTVKNALQKHKGVYLAAVGGAGALIKESIVDYQVIAFEDLGPEAIRRLEV
KNFPVLVANDIYGNDLYQQGKNRYAK
>Mature_186_residues
MRDQFYKKLSAPLAEEEVQELKVGDQVLLSGSVLTARDASHKRLIELKKAGIELPVSLEGEIIYYVGPTPAKPGHVIGSA
GPTTSSRMDRYTTQMLELGAKAFIGKGYRSQTVKNALQKHKGVYLAAVGGAGALIKESIVDYQVIAFEDLGPEAIRRLEV
KNFPVLVANDIYGNDLYQQGKNRYAK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=179, Percent_Identity=38.5474860335196, Blast_Score=126, Evalue=8e-31,
Organism=Escherichia coli, GI1787897, Length=175, Percent_Identity=36.5714285714286, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1789443, Length=185, Percent_Identity=33.5135135135135, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 20367; Mature: 20367

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDQFYKKLSAPLAEEEVQELKVGDQVLLSGSVLTARDASHKRLIELKKAGIELPVSLEG
CCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCCEEECC
EIIYYVGPTPAKPGHVIGSAGPTTSSRMDRYTTQMLELGAKAFIGKGYRSQTVKNALQKH
CEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KGVYLAAVGGAGALIKESIVDYQVIAFEDLGPEAIRRLEVKNFPVLVANDIYGNDLYQQG
CCEEEEEECCCCHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCHHHHHH
KNRYAK
HHCCCC
>Mature Secondary Structure
MRDQFYKKLSAPLAEEEVQELKVGDQVLLSGSVLTARDASHKRLIELKKAGIELPVSLEG
CCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCCEEECC
EIIYYVGPTPAKPGHVIGSAGPTTSSRMDRYTTQMLELGAKAFIGKGYRSQTVKNALQKH
CEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KGVYLAAVGGAGALIKESIVDYQVIAFEDLGPEAIRRLEVKNFPVLVANDIYGNDLYQQG
CCEEEEEECCCCHHHHHHHHCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCHHHHHH
KNRYAK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]