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The map label for this gene is thiI

Identifier: 188586959

GI number: 188586959

Start: 2501579

End: 2502820

Strand: Reverse

Name: thiI

Synonym: Nther_2351

Alternate gene names: 188586959

Gene position: 2502820-2501579 (Counterclockwise)

Preceding gene: 188586960

Following gene: 188586958

Centisome position: 79.06

GC content: 39.69

Gene sequence:

>1242_bases
ATGTACAACTATTTACTGATTCGTTACGGTGAAATAGGCTTAAAGGGTAAAAACAGATCGTATTTTGAAAAGAGCTTGGT
AAAAAATATGCAGTCAGCCTTAAAAGATTTAGAGATAGGTAAGATTAAATCTACCCAGGGACGCATGTATATACCTCTAA
GTGGTAGTAGTGATGAATTGACTAGAGTTTTAGACAGGGTGACCAGGGTCTTTGGAATCGAAACCGTCAGCCCTGCCGTA
AAAGTGGAGTCCGATTTGGAAGTCATCAAAAAGACGGCTTTACAAGTCTTTAAAAATCACATGGATAGTATAAGCCCACA
AAATCAGGTTTCTTTTAAAGTGGATTGTCGCCGAGCTGATAAATTATTTTCCAAAAATTCCATGGAAATGAATCAGATTT
TGGGTGCACACATACTGGACCATGTTCCAGGTCTGAAGGTAGATGTAAAGCAACCACAGATTCTTCTTCAGGTAGAAATT
AGAGAAGATGGGACCTATATTTTTACGGAAAAAATTCCAGGACATGGTGGTCTACCTATTGGTACTACTGGTAAAGGAGT
GTTAATGTTATCTGGAGGTATAGACAGTCCTGTTGCAGGATGGCTTGCCATGAAAAGAGGGATTCAAGTAGTTGGTCTTC
ATTTTCACAGTTACCCCTTTACCAGTCAGCGGGCTTTAAAAAAAGTTGAAGATATCTCCCAAGTTCTTTCACGTTACGGT
ACAGGTCCAACAGGTGGCTTTAAGTTGATTACCAATCACTTCACCGATATCCAAAAAGCCATTCAGAATTACTGCAGTGA
AAGCATGTGGGTTACAGTAATGCGCAGATTCATGTTTTATATAGCTAATCGAATGGCTCAGAAAGAACAAGCAATGACAG
TGGTCACGGGTGAAAATGTTGGGCAGGTAGCTAGCCAAACCCTTGAAAGTATGCATGCAGTCAGTCAAGATGTTGTAAAC
CTTCCCATTTTGCGTCCCCTGGCTGGATTGGATAAAAAAGAGATCATGAGCAAGGCTGAAACTATTGGCACCTACGATAT
ATCTATCCGTCCTTATGAGGACTGCTGTACATTGTTTTTACCTAAAAATCCTAAAACTCGTCCTAGTCTGGAACAAACTA
AGAGGGAAATAAGCAAACTGAACTTTGAGGAACTGGTGGAAGAATCCCTGGAGAAAACTGAAATTAAGTATTTTGAACCT
TATCAGGACATTACAGAGGAAGAATTGACCTTTGATGTTTAA

Upstream 100 bases:

>100_bases
AGCTTTTCATATCATCATACCATGGATGAAATAGATTATGCGGCAGATCAGGTGATAGGAGTTTATCAAGAGTTAAAGCA
ATATGGCCGGAGGTGATAAT

Downstream 100 bases:

>100_bases
GTTCATCTGATGGGAGAGGAGTAGAATAGAAGGAGGGGACATATGAATCTAGATTATTTTGATTCCTTTTTTGTCGTAGT
GGAAGAAGGCAGTATTACTA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MYNYLLIRYGEIGLKGKNRSYFEKSLVKNMQSALKDLEIGKIKSTQGRMYIPLSGSSDELTRVLDRVTRVFGIETVSPAV
KVESDLEVIKKTALQVFKNHMDSISPQNQVSFKVDCRRADKLFSKNSMEMNQILGAHILDHVPGLKVDVKQPQILLQVEI
REDGTYIFTEKIPGHGGLPIGTTGKGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYG
TGPTGGFKLITNHFTDIQKAIQNYCSESMWVTVMRRFMFYIANRMAQKEQAMTVVTGENVGQVASQTLESMHAVSQDVVN
LPILRPLAGLDKKEIMSKAETIGTYDISIRPYEDCCTLFLPKNPKTRPSLEQTKREISKLNFEELVEESLEKTEIKYFEP
YQDITEEELTFDV

Sequences:

>Translated_413_residues
MYNYLLIRYGEIGLKGKNRSYFEKSLVKNMQSALKDLEIGKIKSTQGRMYIPLSGSSDELTRVLDRVTRVFGIETVSPAV
KVESDLEVIKKTALQVFKNHMDSISPQNQVSFKVDCRRADKLFSKNSMEMNQILGAHILDHVPGLKVDVKQPQILLQVEI
REDGTYIFTEKIPGHGGLPIGTTGKGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYG
TGPTGGFKLITNHFTDIQKAIQNYCSESMWVTVMRRFMFYIANRMAQKEQAMTVVTGENVGQVASQTLESMHAVSQDVVN
LPILRPLAGLDKKEIMSKAETIGTYDISIRPYEDCCTLFLPKNPKTRPSLEQTKREISKLNFEELVEESLEKTEIKYFEP
YQDITEEELTFDV
>Mature_413_residues
MYNYLLIRYGEIGLKGKNRSYFEKSLVKNMQSALKDLEIGKIKSTQGRMYIPLSGSSDELTRVLDRVTRVFGIETVSPAV
KVESDLEVIKKTALQVFKNHMDSISPQNQVSFKVDCRRADKLFSKNSMEMNQILGAHILDHVPGLKVDVKQPQILLQVEI
REDGTYIFTEKIPGHGGLPIGTTGKGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYG
TGPTGGFKLITNHFTDIQKAIQNYCSESMWVTVMRRFMFYIANRMAQKEQAMTVVTGENVGQVASQTLESMHAVSQDVVN
LPILRPLAGLDKKEIMSKAETIGTYDISIRPYEDCCTLFLPKNPKTRPSLEQTKREISKLNFEELVEESLEKTEIKYFEP
YQDITEEELTFDV

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=393, Percent_Identity=24.6819338422392, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_NATTJ (B2A8M8)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918504.1
- ProteinModelPortal:   B2A8M8
- SMR:   B2A8M8
- GeneID:   6316938
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2351
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 46621; Mature: 46621

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS51165 THUMP

Important sites: BINDING 275-275 BINDING 297-297 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNYLLIRYGEIGLKGKNRSYFEKSLVKNMQSALKDLEIGKIKSTQGRMYIPLSGSSDEL
CCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCHHHH
TRVLDRVTRVFGIETVSPAVKVESDLEVIKKTALQVFKNHMDSISPQNQVSFKVDCRRAD
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
KLFSKNSMEMNQILGAHILDHVPGLKVDVKQPQILLQVEIREDGTYIFTEKIPGHGGLPI
HHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCC
GTTGKGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYG
CCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
TGPTGGFKLITNHFTDIQKAIQNYCSESMWVTVMRRFMFYIANRMAQKEQAMTVVTGENV
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
GQVASQTLESMHAVSQDVVNLPILRPLAGLDKKEIMSKAETIGTYDISIRPYEDCCTLFL
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHEEEE
PKNPKTRPSLEQTKREISKLNFEELVEESLEKTEIKYFEPYQDITEEELTFDV
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCHHHCCHHHHCCCC
>Mature Secondary Structure
MYNYLLIRYGEIGLKGKNRSYFEKSLVKNMQSALKDLEIGKIKSTQGRMYIPLSGSSDEL
CCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCHHHH
TRVLDRVTRVFGIETVSPAVKVESDLEVIKKTALQVFKNHMDSISPQNQVSFKVDCRRAD
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHH
KLFSKNSMEMNQILGAHILDHVPGLKVDVKQPQILLQVEIREDGTYIFTEKIPGHGGLPI
HHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCC
GTTGKGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYG
CCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
TGPTGGFKLITNHFTDIQKAIQNYCSESMWVTVMRRFMFYIANRMAQKEQAMTVVTGENV
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
GQVASQTLESMHAVSQDVVNLPILRPLAGLDKKEIMSKAETIGTYDISIRPYEDCCTLFL
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCEEEEEECCHHHHHEEEE
PKNPKTRPSLEQTKREISKLNFEELVEESLEKTEIKYFEPYQDITEEELTFDV
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA