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The map label for this gene is cysL [H]

Identifier: 188586958

GI number: 188586958

Start: 2500610

End: 2501536

Strand: Reverse

Name: cysL [H]

Synonym: Nther_2350

Alternate gene names: 188586958

Gene position: 2501536-2500610 (Counterclockwise)

Preceding gene: 188586959

Following gene: 188586957

Centisome position: 79.02

GC content: 35.06

Gene sequence:

>927_bases
ATGAATCTAGATTATTTTGATTCCTTTTTTGTCGTAGTGGAAGAAGGCAGTATTACTAAAGCTGCTAACAAGCTACACAT
TAGTCAACCTGCCCTAAGTGCACAAATTAATTGTCTAGAAAAGTATTTTCAGGTTTCCCTGGTAGAAAGATCTTCTAAAG
GGGTTAAATTGACACCTGCCGGGGAAACCCTCTATGAAGAAGGTAAAAGATTTACCAGTTTATTTAAGAGTATGAGTAAT
AAAATTAGGGAGCACGCCGGTGAAGGCACTAAAAAATTATATATTGGTGCTTCTTCCACTATAGGTAATCATGCTCTGCC
CTGTAGTATATTTAACTTTAAAAAACGTTATCCTCAATATGACATCAAAATGAAGATAACTAATAGTGAAGAAGTAGCCA
ATTTGGTGATGGAAGATAAAGTTGGAATGGGAATAATTGAAGGTCCTGTAGACAAAGAACTACGGGAAGAATTCAAAATA
AGTGGGATTCAAACTAAGCGGTTGTTGTACGATAGATTGAATGTACTAGCCCCCTATGGACCCCCCTGGGCCAAGCGGGA
TTTTATTACAATAGAAGAACTTTTTAATCAAGATTTAATTTTGAGAGAAAAAAGTTCCGGGATTAGAAAAACTATTGAAC
AAACACTAACTAATCAAGGTTTAGACCCATCTAGACTAAATATGAATATGGAGTTGAACAACTCTTGTGCTATTATCAAT
GCAGTTAAAACTGGAAAAGGCCTGTCCATCCTACCTAAAAATGCTTTTAAACAGGATTTGGTTTATGAAGCGTTATCTTC
CCTTGTAGTAGAAGGGGTTGAGTTTTATCATCCTTACACACTTCTATATCATCCTTCCAGATTGAAAGATTTTCTTCACA
GAGAATTTTTTGAACTACTGATTAATGAAGAATATTGCTTTTGTTAA

Upstream 100 bases:

>100_bases
TAAGTATTTTGAACCTTATCAGGACATTACAGAGGAAGAATTGACCTTTGATGTTTAAGTTCATCTGATGGGAGAGGAGT
AGAATAGAAGGAGGGGACAT

Downstream 100 bases:

>100_bases
AAAATATTGTTATTGGTCCAAAAAGCGTGAAGGTGGAAAATAGTCAAAAAGTTTGGTATTCTTTTCCATGAAGGGACTAA
TATTTACCAAAATATAAGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNLDYFDSFFVVVEEGSITKAANKLHISQPALSAQINCLEKYFQVSLVERSSKGVKLTPAGETLYEEGKRFTSLFKSMSN
KIREHAGEGTKKLYIGASSTIGNHALPCSIFNFKKRYPQYDIKMKITNSEEVANLVMEDKVGMGIIEGPVDKELREEFKI
SGIQTKRLLYDRLNVLAPYGPPWAKRDFITIEELFNQDLILREKSSGIRKTIEQTLTNQGLDPSRLNMNMELNNSCAIIN
AVKTGKGLSILPKNAFKQDLVYEALSSLVVEGVEFYHPYTLLYHPSRLKDFLHREFFELLINEEYCFC

Sequences:

>Translated_308_residues
MNLDYFDSFFVVVEEGSITKAANKLHISQPALSAQINCLEKYFQVSLVERSSKGVKLTPAGETLYEEGKRFTSLFKSMSN
KIREHAGEGTKKLYIGASSTIGNHALPCSIFNFKKRYPQYDIKMKITNSEEVANLVMEDKVGMGIIEGPVDKELREEFKI
SGIQTKRLLYDRLNVLAPYGPPWAKRDFITIEELFNQDLILREKSSGIRKTIEQTLTNQGLDPSRLNMNMELNNSCAIIN
AVKTGKGLSILPKNAFKQDLVYEALSSLVVEGVEFYHPYTLLYHPSRLKDFLHREFFELLINEEYCFC
>Mature_308_residues
MNLDYFDSFFVVVEEGSITKAANKLHISQPALSAQINCLEKYFQVSLVERSSKGVKLTPAGETLYEEGKRFTSLFKSMSN
KIREHAGEGTKKLYIGASSTIGNHALPCSIFNFKKRYPQYDIKMKITNSEEVANLVMEDKVGMGIIEGPVDKELREEFKI
SGIQTKRLLYDRLNVLAPYGPPWAKRDFITIEELFNQDLILREKSSGIRKTIEQTLTNQGLDPSRLNMNMELNNSCAIIN
AVKTGKGLSILPKNAFKQDLVYEALSSLVVEGVEFYHPYTLLYHPSRLKDFLHREFFELLINEEYCFC

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=277, Percent_Identity=32.8519855595668, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=25.5725190839695, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=262, Percent_Identity=26.3358778625954, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=252, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789506, Length=241, Percent_Identity=26.1410788381743, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=24.609375, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787806, Length=255, Percent_Identity=22.3529411764706, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789204, Length=299, Percent_Identity=25.752508361204, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=240, Percent_Identity=24.1666666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=259, Percent_Identity=23.1660231660232, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=21.6494845360825, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=138, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=254, Percent_Identity=24.0157480314961, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35136; Mature: 35136

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDYFDSFFVVVEEGSITKAANKLHISQPALSAQINCLEKYFQVSLVERSSKGVKLTPA
CCCCCCCCEEEEEECCCCHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GETLYEEGKRFTSLFKSMSNKIREHAGEGTKKLYIGASSTIGNHALPCSIFNFKKRYPQY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCC
DIKMKITNSEEVANLVMEDKVGMGIIEGPVDKELREEFKISGIQTKRLLYDRLNVLAPYG
EEEEEECCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PPWAKRDFITIEELFNQDLILREKSSGIRKTIEQTLTNQGLDPSRLNMNMELNNSCAIIN
CCCCCCCCEEHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCEEEEE
AVKTGKGLSILPKNAFKQDLVYEALSSLVVEGVEFYHPYTLLYHPSRLKDFLHREFFELL
EEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHH
INEEYCFC
HCCCCCCC
>Mature Secondary Structure
MNLDYFDSFFVVVEEGSITKAANKLHISQPALSAQINCLEKYFQVSLVERSSKGVKLTPA
CCCCCCCCEEEEEECCCCHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
GETLYEEGKRFTSLFKSMSNKIREHAGEGTKKLYIGASSTIGNHALPCSIFNFKKRYPQY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCC
DIKMKITNSEEVANLVMEDKVGMGIIEGPVDKELREEFKISGIQTKRLLYDRLNVLAPYG
EEEEEECCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
PPWAKRDFITIEELFNQDLILREKSSGIRKTIEQTLTNQGLDPSRLNMNMELNNSCAIIN
CCCCCCCCEEHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCCEEEEE
AVKTGKGLSILPKNAFKQDLVYEALSSLVVEGVEFYHPYTLLYHPSRLKDFLHREFFELL
EEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHH
INEEYCFC
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]