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The map label for this gene is iscS [H]

Identifier: 188586960

GI number: 188586960

Start: 2502825

End: 2503991

Strand: Reverse

Name: iscS [H]

Synonym: Nther_2352

Alternate gene names: 188586960

Gene position: 2503991-2502825 (Counterclockwise)

Preceding gene: 188586961

Following gene: 188586959

Centisome position: 79.1

GC content: 39.42

Gene sequence:

>1167_bases
ATGGGGTCAAGAAAAATTTATCTGGATAATAGTGCTACAACCAAAGTTGATAAAGAAGTGGCTGATGTTGTTTATAAAAT
AATGACAGAAGGGTATGGTAATCCTAGTTCTTTGCATGGTTTAGGTGTTACCGCTGAAGATAATATTACGAAAGTACGCT
CTTTTTTAATGAAAGCTCTGAAAGCCTCACAGGGTGAGCTAATATTTACTTCTGGAGGTACCGAAGCTAACAATCTGGCT
ATTAGAGGTGTGGCTTATCGAGAAAACAGGCAAGGTAATCATCTGATAACTACAGAAGTGGAACATCCCTCGGTTTTAGA
AGTTTTTAACAGTTTAGAAGATCAGGGATATCAAGTCACTTATCTGCCAGTTGATCATAAAGGTATAATTGACCTTTACC
AATTGCAACAAGCTGTTAATGACGATACTATTTTAGTCAGTATTCAGGCCGTCAATAACGAAACGGGGACGATTCAACCG
GTCAAAGAAGCAGCGAAACTGGTTAAGGACGTTTCGCATAAACCTGTATTTCATTGTGATGCCGTACAGGCAGTTGGTAA
AATTGAGCTTGCTCCCGAGGACTGGGGAATTGATCTGTTGACTGTAAGTGCCCATAAATTTCATGGCCCCAAGGGTGTGG
GTAGTCTGTATATTTCTCCTAGAACCAGATTAAAACCTTTGTTCTATGGTGGTGGTCAGGAACAGGACTTGCGACCAGGA
ACAGAAAATGTCCCTGGAATAACAGGTATGGGAAAAGCTCTGGAAAAAATGCTAAAGAACAGGGAACAATTTAAAGAACT
CTATTCTTTGAAAAAACACTTATATGATAAAATCACCTTAAAACTAGGAAATGAAGTCATGCTGATAGGTCCTGAAATTC
AGGAAGGTGTTCCCTATATCATGTCTCTGGCATTTTCTGGAGTCAAAGCGGAAGTTATGATTCACGCCCTGGAAAAAAGA
GGTGTGTATGTATCCGCTGGGTCTGCCTGTACTTCTAATAAAGAAGGTGAAAGTCATGTGTTAAAAGCTATGAAAATTCC
AGATGATTTTAGAGAAGGTACCATTAGATTGAGCTTTTCATATCATCATACCATGGATGAAATAGATTATGCGGCAGATC
AGGTGATAGGAGTTTATCAAGAGTTAAAGCAATATGGCCGGAGGTGA

Upstream 100 bases:

>100_bases
GATTTTTCTATTAATATAACTTATAGTATAATTAAGGTAAATTCAGCTATTTAAATGAGAAACTAATTCTCTACAATTTA
TTAAAATTGAGGTGTCAATC

Downstream 100 bases:

>100_bases
TAATATGTACAACTATTTACTGATTCGTTACGGTGAAATAGGCTTAAAGGGTAAAAACAGATCGTATTTTGAAAAGAGCT
TGGTAAAAAATATGCAGTCA

Product: aminotransferase class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MGSRKIYLDNSATTKVDKEVADVVYKIMTEGYGNPSSLHGLGVTAEDNITKVRSFLMKALKASQGELIFTSGGTEANNLA
IRGVAYRENRQGNHLITTEVEHPSVLEVFNSLEDQGYQVTYLPVDHKGIIDLYQLQQAVNDDTILVSIQAVNNETGTIQP
VKEAAKLVKDVSHKPVFHCDAVQAVGKIELAPEDWGIDLLTVSAHKFHGPKGVGSLYISPRTRLKPLFYGGGQEQDLRPG
TENVPGITGMGKALEKMLKNREQFKELYSLKKHLYDKITLKLGNEVMLIGPEIQEGVPYIMSLAFSGVKAEVMIHALEKR
GVYVSAGSACTSNKEGESHVLKAMKIPDDFREGTIRLSFSYHHTMDEIDYAADQVIGVYQELKQYGRR

Sequences:

>Translated_388_residues
MGSRKIYLDNSATTKVDKEVADVVYKIMTEGYGNPSSLHGLGVTAEDNITKVRSFLMKALKASQGELIFTSGGTEANNLA
IRGVAYRENRQGNHLITTEVEHPSVLEVFNSLEDQGYQVTYLPVDHKGIIDLYQLQQAVNDDTILVSIQAVNNETGTIQP
VKEAAKLVKDVSHKPVFHCDAVQAVGKIELAPEDWGIDLLTVSAHKFHGPKGVGSLYISPRTRLKPLFYGGGQEQDLRPG
TENVPGITGMGKALEKMLKNREQFKELYSLKKHLYDKITLKLGNEVMLIGPEIQEGVPYIMSLAFSGVKAEVMIHALEKR
GVYVSAGSACTSNKEGESHVLKAMKIPDDFREGTIRLSFSYHHTMDEIDYAADQVIGVYQELKQYGRR
>Mature_387_residues
GSRKIYLDNSATTKVDKEVADVVYKIMTEGYGNPSSLHGLGVTAEDNITKVRSFLMKALKASQGELIFTSGGTEANNLAI
RGVAYRENRQGNHLITTEVEHPSVLEVFNSLEDQGYQVTYLPVDHKGIIDLYQLQQAVNDDTILVSIQAVNNETGTIQPV
KEAAKLVKDVSHKPVFHCDAVQAVGKIELAPEDWGIDLLTVSAHKFHGPKGVGSLYISPRTRLKPLFYGGGQEQDLRPGT
ENVPGITGMGKALEKMLKNREQFKELYSLKKHLYDKITLKLGNEVMLIGPEIQEGVPYIMSLAFSGVKAEVMIHALEKRG
VYVSAGSACTSNKEGESHVLKAMKIPDDFREGTIRLSFSYHHTMDEIDYAADQVIGVYQELKQYGRR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=40.5194805194805, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=33.7349397590361, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI48994898, Length=379, Percent_Identity=39.5778364116095, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1787970, Length=394, Percent_Identity=25.1269035532995, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=22.4181360201511, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=376, Percent_Identity=38.563829787234, Blast_Score=250, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=34.3465045592705, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=39.3139841688654, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=40.2597402597403, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42927; Mature: 42795

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSRKIYLDNSATTKVDKEVADVVYKIMTEGYGNPSSLHGLGVTAEDNITKVRSFLMKAL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHH
KASQGELIFTSGGTEANNLAIRGVAYRENRQGNHLITTEVEHPSVLEVFNSLEDQGYQVT
HCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEE
YLPVDHKGIIDLYQLQQAVNDDTILVSIQAVNNETGTIQPVKEAAKLVKDVSHKPVFHCD
EEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEH
AVQAVGKIELAPEDWGIDLLTVSAHKFHGPKGVGSLYISPRTRLKPLFYGGGQEQDLRPG
HHHHCCEEEECCCCCCEEEEEEECHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCC
TENVPGITGMGKALEKMLKNREQFKELYSLKKHLYDKITLKLGNEVMLIGPEIQEGVPYI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCHHHCCHHH
MSLAFSGVKAEVMIHALEKRGVYVSAGSACTSNKEGESHVLKAMKIPDDFREGTIRLSFS
HHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
YHHTMDEIDYAADQVIGVYQELKQYGRR
ECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GSRKIYLDNSATTKVDKEVADVVYKIMTEGYGNPSSLHGLGVTAEDNITKVRSFLMKAL
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHH
KASQGELIFTSGGTEANNLAIRGVAYRENRQGNHLITTEVEHPSVLEVFNSLEDQGYQVT
HCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEE
YLPVDHKGIIDLYQLQQAVNDDTILVSIQAVNNETGTIQPVKEAAKLVKDVSHKPVFHCD
EEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEH
AVQAVGKIELAPEDWGIDLLTVSAHKFHGPKGVGSLYISPRTRLKPLFYGGGQEQDLRPG
HHHHCCEEEECCCCCCEEEEEEECHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCC
TENVPGITGMGKALEKMLKNREQFKELYSLKKHLYDKITLKLGNEVMLIGPEIQEGVPYI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCHHHCCHHH
MSLAFSGVKAEVMIHALEKRGVYVSAGSACTSNKEGESHVLKAMKIPDDFREGTIRLSFS
HHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
YHHTMDEIDYAADQVIGVYQELKQYGRR
ECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA