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The map label for this gene is leuD1 [H]

Identifier: 188586767

GI number: 188586767

Start: 2284330

End: 2284887

Strand: Reverse

Name: leuD1 [H]

Synonym: Nther_2158

Alternate gene names: 188586767

Gene position: 2284887-2284330 (Counterclockwise)

Preceding gene: 188586768

Following gene: 188586766

Centisome position: 72.18

GC content: 37.63

Gene sequence:

>558_bases
ATGAATAAGACAAGAGAGACAAGGAAAGAGTTTTCGCCGATGCAAGATGAAAGGTTTAGAGGAAAAGCTTTTGTATTGGG
GGATAATATAGATACAGATCAAATTTTACCAGGATACGCCATGTCAGCACCAAAGGAACAGCTGAGACACTACGTTTTAA
AAGGTAGTTCAATACCTGATTTTTCGGAGCGAGTACAGCAGGGTGACATTATCATAGGTGGAGACAATTTTGGTTGTGGT
TCTAGCAGGGAGCAAGCTCCTATAGCTTTAAAAGATGCGGGTGTTTCAGCAATTATTGCTAAATCTTTTGCCAGGATTTT
TAGAAGGAATGCAATAAATATCGGTCTTCCTGTATTATATGGTGATGTGGTGGAAGAAATAGAAACAGGGGATGAAGTGC
AATTAAAAATATATCAGTATAAGTTGGAAAATATCACAAAAGATAAAACTTTCCAACTAGAGGAGCTATCACAAGTCACC
ATGGAAACACTGGAAGCCGGTGGACTGATCAACAAAGTCAAAATGATATTAAATATAAGAGGTGATATGAATGAGTAG

Upstream 100 bases:

>100_bases
AGGATGGGACATCCTGAAGCCGAAATTTATTTGGTCTCTCCTCGAACAGCAGTTGAGAGCGCTTTGACAGGAAAAATCAC
ATTATCAAAGGAGCGGTTAA

Downstream 100 bases:

>100_bases
TGAAAGGCCAAATACTAAGCGAATTATTCGTGAAGATATTGACAGAATAAGAGCTTATGAAAAGGCTTTTGATGTGGATA
TGCCCTATATTGGCGAGGAC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNKTRETRKEFSPMQDERFRGKAFVLGDNIDTDQILPGYAMSAPKEQLRHYVLKGSSIPDFSERVQQGDIIIGGDNFGCG
SSREQAPIALKDAGVSAIIAKSFARIFRRNAINIGLPVLYGDVVEEIETGDEVQLKIYQYKLENITKDKTFQLEELSQVT
METLEAGGLINKVKMILNIRGDMNE

Sequences:

>Translated_185_residues
MNKTRETRKEFSPMQDERFRGKAFVLGDNIDTDQILPGYAMSAPKEQLRHYVLKGSSIPDFSERVQQGDIIIGGDNFGCG
SSREQAPIALKDAGVSAIIAKSFARIFRRNAINIGLPVLYGDVVEEIETGDEVQLKIYQYKLENITKDKTFQLEELSQVT
METLEAGGLINKVKMILNIRGDMNE
>Mature_185_residues
MNKTRETRKEFSPMQDERFRGKAFVLGDNIDTDQILPGYAMSAPKEQLRHYVLKGSSIPDFSERVQQGDIIIGGDNFGCG
SSREQAPIALKDAGVSAIIAKSFARIFRRNAINIGLPVLYGDVVEEIETGDEVQLKIYQYKLENITKDKTFQLEELSQVT
METLEAGGLINKVKMILNIRGDMNE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=42.7083333333333, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20748; Mature: 20748

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTRETRKEFSPMQDERFRGKAFVLGDNIDTDQILPGYAMSAPKEQLRHYVLKGSSIPD
CCCCHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
FSERVQQGDIIIGGDNFGCGSSREQAPIALKDAGVSAIIAKSFARIFRRNAINIGLPVLY
HHHHHHCCCEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHH
GDVVEEIETGDEVQLKIYQYKLENITKDKTFQLEELSQVTMETLEAGGLINKVKMILNIR
HHHHHHHCCCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEC
GDMNE
CCCCC
>Mature Secondary Structure
MNKTRETRKEFSPMQDERFRGKAFVLGDNIDTDQILPGYAMSAPKEQLRHYVLKGSSIPD
CCCCHHHHHHCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
FSERVQQGDIIIGGDNFGCGSSREQAPIALKDAGVSAIIAKSFARIFRRNAINIGLPVLY
HHHHHHCCCEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHH
GDVVEEIETGDEVQLKIYQYKLENITKDKTFQLEELSQVTMETLEAGGLINKVKMILNIR
HHHHHHHCCCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEC
GDMNE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]