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The map label for this gene is leuC1 [H]

Identifier: 188586768

GI number: 188586768

Start: 2284880

End: 2286076

Strand: Reverse

Name: leuC1 [H]

Synonym: Nther_2159

Alternate gene names: 188586768

Gene position: 2286076-2284880 (Counterclockwise)

Preceding gene: 188586769

Following gene: 188586767

Centisome position: 72.22

GC content: 41.69

Gene sequence:

>1197_bases
ATGAATATCATTGAAAGATTAATAGCAGAAAAAGCTGGTAGAGATCATGTTGAAGTAGGGGAAGAAGTCTCTTGCTATGC
AGACCTTGTAGTTGCCCACGATGTAACCGGACCATTAGCTGTAGAAAGATTTTACGATATAGGAACAGATAGACTATTTG
ATCCCAAGAAAGTAGCCTTTGTTATTGATCACAACATTCCGGCTTCATCTGTACAGTCACGTCAGCAGCATAAATCAGTG
AAAGAATTCGCTAAAAAGACAGGAGCTAGATTATACGATCGAAGTGACGGTGTGATCCATCAGGTAATTTATGAACAGGG
CCTCTATCAAAAAGGCCAACTGGTAGTTGGAGCCGATTCTCACACCTGCACAGCCGGTGCATCTGGGGCTTTATCTGTAG
CTGTTGGTTCTACAGAACTGGCCGCTGTTATGGCTCTTGGAACCATCGATTTGGAAGTACCGGACACTTACCTGATTCAA
ATCGATGGAGAGTTACCTCAAGGAGTATACGCTAAAGATATCATTTTAACTATTCTAGGTAAGTTTGGGACTAATGGTTT
TACTGATAAAGCTGTGATTTTTGGCGGTGGTGGTATAACGAATTTGAGTATAGATGAGAAAATGACTATTTCAAATATGG
CTATTGAAATGGGAGCTATGATTGGATATGTGGATCAAGGGGAAGAAATTGGTTCTGTTAAGGAAAGCTATACAATAAAA
GCTTCCGAATTAGAACCCGTGGCTGCTTGCCCTGCTTCTCCCGGAAATGTTAAGCCAATTTCACAAGTGGCCGGGACCGA
AATTGACCAAGTAGTTATAGGTAGCTGTACTAACGGAAGGTTCAGTGATATGGAAATAGCTGCCAAAGTACTAGATGGGA
AAAGAATTTCCGAAAATGTTGATATGATAGTGATTCCAGCTTCTGAAAAAGTGTTGGTTGAAATGGAAAAGAGAGAAATT
ACCGAAACACTTAGAAACGCAGGGGCAGTGATTACAAACCCTGGTTGTGGACCTTGTTATGGTGCTCATCAAGGTTTACT
GGCACCTGGAGAGGTTGGTCTATCGACTACCAATAGAAATTTCCCCGGGAGGATGGGACATCCTGAAGCCGAAATTTATT
TGGTCTCTCCTCGAACAGCAGTTGAGAGCGCTTTGACAGGAAAAATCACATTATCAAAGGAGCGGTTAAATGAATAA

Upstream 100 bases:

>100_bases
AGGCCAAACAAATAGCGGAAGACTTGGGCATAGTTCTGGTGGATTAATAACATGACTAATTTAAACAATAAAATGATCAG
GGAAAGAAGGTGAAACTTTC

Downstream 100 bases:

>100_bases
GACAAGAGAGACAAGGAAAGAGTTTTCGCCGATGCAAGATGAAAGGTTTAGAGGAAAAGCTTTTGTATTGGGGGATAATA
TAGATACAGATCAAATTTTA

Product: 3-isopropylmalate dehydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNIIERLIAEKAGRDHVEVGEEVSCYADLVVAHDVTGPLAVERFYDIGTDRLFDPKKVAFVIDHNIPASSVQSRQQHKSV
KEFAKKTGARLYDRSDGVIHQVIYEQGLYQKGQLVVGADSHTCTAGASGALSVAVGSTELAAVMALGTIDLEVPDTYLIQ
IDGELPQGVYAKDIILTILGKFGTNGFTDKAVIFGGGGITNLSIDEKMTISNMAIEMGAMIGYVDQGEEIGSVKESYTIK
ASELEPVAACPASPGNVKPISQVAGTEIDQVVIGSCTNGRFSDMEIAAKVLDGKRISENVDMIVIPASEKVLVEMEKREI
TETLRNAGAVITNPGCGPCYGAHQGLLAPGEVGLSTTNRNFPGRMGHPEAEIYLVSPRTAVESALTGKITLSKERLNE

Sequences:

>Translated_398_residues
MNIIERLIAEKAGRDHVEVGEEVSCYADLVVAHDVTGPLAVERFYDIGTDRLFDPKKVAFVIDHNIPASSVQSRQQHKSV
KEFAKKTGARLYDRSDGVIHQVIYEQGLYQKGQLVVGADSHTCTAGASGALSVAVGSTELAAVMALGTIDLEVPDTYLIQ
IDGELPQGVYAKDIILTILGKFGTNGFTDKAVIFGGGGITNLSIDEKMTISNMAIEMGAMIGYVDQGEEIGSVKESYTIK
ASELEPVAACPASPGNVKPISQVAGTEIDQVVIGSCTNGRFSDMEIAAKVLDGKRISENVDMIVIPASEKVLVEMEKREI
TETLRNAGAVITNPGCGPCYGAHQGLLAPGEVGLSTTNRNFPGRMGHPEAEIYLVSPRTAVESALTGKITLSKERLNE
>Mature_398_residues
MNIIERLIAEKAGRDHVEVGEEVSCYADLVVAHDVTGPLAVERFYDIGTDRLFDPKKVAFVIDHNIPASSVQSRQQHKSV
KEFAKKTGARLYDRSDGVIHQVIYEQGLYQKGQLVVGADSHTCTAGASGALSVAVGSTELAAVMALGTIDLEVPDTYLIQ
IDGELPQGVYAKDIILTILGKFGTNGFTDKAVIFGGGGITNLSIDEKMTISNMAIEMGAMIGYVDQGEEIGSVKESYTIK
ASELEPVAACPASPGNVKPISQVAGTEIDQVVIGSCTNGRFSDMEIAAKVLDGKRISENVDMIVIPASEKVLVEMEKREI
TETLRNAGAVITNPGCGPCYGAHQGLLAPGEVGLSTTNRNFPGRMGHPEAEIYLVSPRTAVESALTGKITLSKERLNE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=420, Percent_Identity=26.6666666666667, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI8659555, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786259, Length=436, Percent_Identity=30.9633027522936, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87081781, Length=375, Percent_Identity=24, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367097, Length=124, Percent_Identity=35.4838709677419, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1787531, Length=210, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=419, Percent_Identity=26.7303102625298, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564738, Length=414, Percent_Identity=26.5700483091787, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25149342, Length=294, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17568399, Length=202, Percent_Identity=31.6831683168317, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=431, Percent_Identity=31.322505800464, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=440, Percent_Identity=29.3181818181818, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6323335, Length=419, Percent_Identity=25.7756563245823, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=421, Percent_Identity=23.2779097387173, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28571643, Length=421, Percent_Identity=26.8408551068884, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281365315, Length=439, Percent_Identity=25.0569476082005, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17864292, Length=439, Percent_Identity=25.0569476082005, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161076999, Length=414, Percent_Identity=25.3623188405797, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24645686, Length=254, Percent_Identity=29.9212598425197, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137564, Length=242, Percent_Identity=30.9917355371901, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 42411; Mature: 42411

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIERLIAEKAGRDHVEVGEEVSCYADLVVAHDVTGPLAVERFYDIGTDRLFDPKKVAF
CCHHHHHHHHHCCCCHHHCCCCHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEE
VIDHNIPASSVQSRQQHKSVKEFAKKTGARLYDRSDGVIHQVIYEQGLYQKGQLVVGADS
EEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCEEEECCC
HTCTAGASGALSVAVGSTELAAVMALGTIDLEVPDTYLIQIDGELPQGVYAKDIILTILG
CCCCCCCCCEEEEEECCHHHHHHHEECEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
KFGTNGFTDKAVIFGGGGITNLSIDEKMTISNMAIEMGAMIGYVDQGEEIGSVKESYTIK
HHCCCCCCCCEEEEECCCEEECCCCCCEEHHHHHHHHHHHEEECCCCCHHCCCHHHEEEE
ASELEPVAACPASPGNVKPISQVAGTEIDQVVIGSCTNGRFSDMEIAAKVLDGKRISENV
ECCCCCHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCC
DMIVIPASEKVLVEMEKREITETLRNAGAVITNPGCGPCYGAHQGLLAPGEVGLSTTNRN
CEEEECCCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
FPGRMGHPEAEIYLVSPRTAVESALTGKITLSKERLNE
CCCCCCCCCCEEEEECCCHHHHHHHCCEEEEEHHHCCC
>Mature Secondary Structure
MNIIERLIAEKAGRDHVEVGEEVSCYADLVVAHDVTGPLAVERFYDIGTDRLFDPKKVAF
CCHHHHHHHHHCCCCHHHCCCCHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEE
VIDHNIPASSVQSRQQHKSVKEFAKKTGARLYDRSDGVIHQVIYEQGLYQKGQLVVGADS
EEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCEEEECCC
HTCTAGASGALSVAVGSTELAAVMALGTIDLEVPDTYLIQIDGELPQGVYAKDIILTILG
CCCCCCCCCEEEEEECCHHHHHHHEECEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
KFGTNGFTDKAVIFGGGGITNLSIDEKMTISNMAIEMGAMIGYVDQGEEIGSVKESYTIK
HHCCCCCCCCEEEEECCCEEECCCCCCEEHHHHHHHHHHHEEECCCCCHHCCCHHHEEEE
ASELEPVAACPASPGNVKPISQVAGTEIDQVVIGSCTNGRFSDMEIAAKVLDGKRISENV
ECCCCCHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCC
DMIVIPASEKVLVEMEKREITETLRNAGAVITNPGCGPCYGAHQGLLAPGEVGLSTTNRN
CEEEECCCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
FPGRMGHPEAEIYLVSPRTAVESALTGKITLSKERLNE
CCCCCCCCCCEEEEECCCHHHHHHHCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]