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The map label for this gene is miaA

Identifier: 188586124

GI number: 188586124

Start: 1572457

End: 1573455

Strand: Direct

Name: miaA

Synonym: Nther_1498

Alternate gene names: 188586124

Gene position: 1572457-1573455 (Clockwise)

Preceding gene: 188586123

Following gene: 188586125

Centisome position: 49.67

GC content: 33.93

Gene sequence:

>999_bases
ATGATGAACACATATGACAATCAACACCGTGAACAACAAAAGTTGAGTGAAAATATATTAAAAACAAAACCTCTTATAGC
AATTGTAGGCCCTACAGCTGTTGGAAAGACGGATATTTCTATAAAAGTAGCCGAAAGATTACCTGTGTCTACTGGAATAA
TTTCGGCTGACTCTATGCAAGTATATAAAAAAATGGATATCGGAACAGCCAAACCTTCCCAAGAAATTAGAACCAAGATA
CCACATTATCTAGTTGATAATGTGGAACCAGACGAAGAATTTAGTGTAGGAAGATATCAAAAACATGCTAAATCAATTCT
GAATCAACTTTATCAAAACAAAGAGCTGCCCCTTTTAGTAGGAGGTACGGGCTTATATGTAGATGCGCTTATTTATGACT
TTTCAATGAATGAGCTTCCTAAATCAACGAAATATCGTCAAGAGTTACAGCAAAAAGCAGAACAAGATGGTTTAGAACAG
TTGTACCGGAAACTAGAAAAAGTAGACCCCGATGCTGCTGATAGAATTCATCCCAATGATCAAAGGAGAATCATTCGCGC
ATTAGAAGTTTATTATTACACTGGTGAACCTATCAGCCAAAGACAAAAGAGAAGATACGACTCTCCTTATAATTTATTAA
TATTTGGCATAACAATGGATAGAAACAAATTATATAACAAAATAGAAACACGTGTAGATCAAATGATTGATCAAGGTTTA
GTGGAGGAAGTAAAGTACTTATTAGAAAACGGTTATCACTTACAGCTCACTTCTATGCAAGGTTTGGGATATAAAGAGAT
AGCAGGGTATTTAATGGGTGATTATGATTTAGAAACTGCAGTTCAAAAGTTAAAGAAAAACACTAAAAGGTTTGCAAAAA
GACAGCTTTCCTGGTTTCGAAGGGATCCCAATATAAAATGGTTAGATATTACTGAAACTGATAAAAGTGAAATTTGTGAC
AAGATTATTGATATGATCTTGAAGGAATATTTGGAATAA

Upstream 100 bases:

>100_bases
AACTTAACCAGAACAGATGTAGAAAAACTGTTTTACCGAGTTTAATAAACTTAGCAGAATGCTATAAAAAATTTTGCTAG
TTATTGGGGTGAAAAAATTT

Downstream 100 bases:

>100_bases
CATAGAAATATAGATATTAGGTAATAAAAATTATATAATATATAAATTATATTGGAGGCGATAGCTTGTCAAAAAACACA
ATTAATTTACAGGATAATGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MMNTYDNQHREQQKLSENILKTKPLIAIVGPTAVGKTDISIKVAERLPVSTGIISADSMQVYKKMDIGTAKPSQEIRTKI
PHYLVDNVEPDEEFSVGRYQKHAKSILNQLYQNKELPLLVGGTGLYVDALIYDFSMNELPKSTKYRQELQQKAEQDGLEQ
LYRKLEKVDPDAADRIHPNDQRRIIRALEVYYYTGEPISQRQKRRYDSPYNLLIFGITMDRNKLYNKIETRVDQMIDQGL
VEEVKYLLENGYHLQLTSMQGLGYKEIAGYLMGDYDLETAVQKLKKNTKRFAKRQLSWFRRDPNIKWLDITETDKSEICD
KIIDMILKEYLE

Sequences:

>Translated_332_residues
MMNTYDNQHREQQKLSENILKTKPLIAIVGPTAVGKTDISIKVAERLPVSTGIISADSMQVYKKMDIGTAKPSQEIRTKI
PHYLVDNVEPDEEFSVGRYQKHAKSILNQLYQNKELPLLVGGTGLYVDALIYDFSMNELPKSTKYRQELQQKAEQDGLEQ
LYRKLEKVDPDAADRIHPNDQRRIIRALEVYYYTGEPISQRQKRRYDSPYNLLIFGITMDRNKLYNKIETRVDQMIDQGL
VEEVKYLLENGYHLQLTSMQGLGYKEIAGYLMGDYDLETAVQKLKKNTKRFAKRQLSWFRRDPNIKWLDITETDKSEICD
KIIDMILKEYLE
>Mature_332_residues
MMNTYDNQHREQQKLSENILKTKPLIAIVGPTAVGKTDISIKVAERLPVSTGIISADSMQVYKKMDIGTAKPSQEIRTKI
PHYLVDNVEPDEEFSVGRYQKHAKSILNQLYQNKELPLLVGGTGLYVDALIYDFSMNELPKSTKYRQELQQKAEQDGLEQ
LYRKLEKVDPDAADRIHPNDQRRIIRALEVYYYTGEPISQRQKRRYDSPYNLLIFGITMDRNKLYNKIETRVDQMIDQGL
VEEVKYLLENGYHLQLTSMQGLGYKEIAGYLMGDYDLETAVQKLKKNTKRFAKRQLSWFRRDPNIKWLDITETDKSEICD
KIIDMILKEYLE

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=30.6709265175719, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=36.3934426229508, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI25144712, Length=281, Percent_Identity=27.4021352313167, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=302, Percent_Identity=31.1258278145695, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24649830, Length=360, Percent_Identity=30.2777777777778, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NATTJ (B2A3X9)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917669.1
- ProteinModelPortal:   B2A3X9
- SMR:   B2A3X9
- GeneID:   6314834
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1498
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 38743; Mature: 38743

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNTYDNQHREQQKLSENILKTKPLIAIVGPTAVGKTDISIKVAERLPVSTGIISADSMQ
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHCCCHHCCCCCHHHH
VYKKMDIGTAKPSQEIRTKIPHYLVDNVEPDEEFSVGRYQKHAKSILNQLYQNKELPLLV
HHHHHCCCCCCCHHHHHHHCCHHHHCCCCCCCHHCHHHHHHHHHHHHHHHHCCCCCCEEE
GGTGLYVDALIYDFSMNELPKSTKYRQELQQKAEQDGLEQLYRKLEKVDPDAADRIHPND
CCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCH
QRRIIRALEVYYYTGEPISQRQKRRYDSPYNLLIFGITMDRNKLYNKIETRVDQMIDQGL
HHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHH
VEEVKYLLENGYHLQLTSMQGLGYKEIAGYLMGDYDLETAVQKLKKNTKRFAKRQLSWFR
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDPNIKWLDITETDKSEICDKIIDMILKEYLE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMNTYDNQHREQQKLSENILKTKPLIAIVGPTAVGKTDISIKVAERLPVSTGIISADSMQ
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHCCCHHCCCCCHHHH
VYKKMDIGTAKPSQEIRTKIPHYLVDNVEPDEEFSVGRYQKHAKSILNQLYQNKELPLLV
HHHHHCCCCCCCHHHHHHHCCHHHHCCCCCCCHHCHHHHHHHHHHHHHHHHCCCCCCEEE
GGTGLYVDALIYDFSMNELPKSTKYRQELQQKAEQDGLEQLYRKLEKVDPDAADRIHPND
CCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCH
QRRIIRALEVYYYTGEPISQRQKRRYDSPYNLLIFGITMDRNKLYNKIETRVDQMIDQGL
HHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHH
VEEVKYLLENGYHLQLTSMQGLGYKEIAGYLMGDYDLETAVQKLKKNTKRFAKRQLSWFR
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDPNIKWLDITETDKSEICDKIIDMILKEYLE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA