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The map label for this gene is rimO

Identifier: 188586093

GI number: 188586093

Start: 1539201

End: 1540535

Strand: Direct

Name: rimO

Synonym: Nther_1467

Alternate gene names: 188586093

Gene position: 1539201-1540535 (Clockwise)

Preceding gene: 188586092

Following gene: 188586094

Centisome position: 48.62

GC content: 33.11

Gene sequence:

>1335_bases
ATGAAAGTAGGGATTATTTCACTAGGATGTGCAAAGAATCAAGTAGATACTGAGGTTATGCAAGGAATTTTAGAAAACAG
CAATTACAAAATGACCGATGATTATTACGATGCCGATATTATTATCGTTAATACTTGTGGTTTTATTGATGATGCCAAAG
AAGAATCTGTTGACCATATTTTAGAAGTTGCTCAATTGAAAGAAACTGGTAAATTGAAAGTTTTGATTGTTGCTGGTTGC
CTATCACAGCGTTATCAAGAATCCCTAAAAGAAGAAATCCCTGAAATCGACGCAATGATAGGTACAGATACCCAAGATAA
AATTACAGAAGTTATTTCATCTGCATTAAAAGGAAATTATATATCATTTTATGATCGATTAAATAAGATAGACGAACAAC
TTTTCTTAAGACAACCATATCAACCTGGGCCTAGCGCTTATATTAAGATTGCTGAAGGCTGTCATAATTACTGTTCTTAC
TGTGCTATACCCTTAATAAGAGGTGGTTATCGCAGTCGTACTATTGAGGATATTAAGATCGAAGCTAATCATTTTATTGA
AAAAGGAAGTAAAGAACTCACCTTAATAGCTCAGGATACTACAAATTACGGTAGTGATATATATGGTAAATTTAGTTTAG
ATACTTTATTAGATGAACTTGCCACAATACCGGGAGATTTTTGGATTAGAGTTCTTTATGCTTATCCCACGAGAATAACT
GATTCCTTAATTGAAGTTATAAATAGACATGAAAAGATCTGTTCTTACTTAGATATCCCATTACAACATATTGATGACGA
TATCTTAACAAGTATGAATAGGGGTGGAAATAAAGAGCAGATCTTGAATTTAATTCATAATTTAAGAAAAAACATTCCAG
ATATTACTCTAAGAACTTCTTTGATAGTAGGTTTTCCCGGTGAAACAGACGAAAAATATCAAAACTTAATTTCATTTATG
CAAGAGATTGAATTTGACCATGCTGGTATTTTTAAGTATTCCGATGAAGAAGATACCCAAGCTTATAATTTTAAAGATAA
AGTCTCGGAAGATGTTAAAGAACAACGTTATCAGGAGGCCTGGGAAGTTCAAAAAGAAATTACAAGGAAAAAGAATGAGG
GTTTGGTAGGAACTGAAATGCGAGTTCTAATTGAAGAAGCCCTAGAAGATGAACCTACTACTAAAGTCGGGCGAACTGAA
GGTCATGCTCCTGAAGTGGATGGAGCTGTTATAATACCCGATTGTGAAGCTTCATCTGGAGATTTTATAAATGTTGAGAT
AGTTCAAGCTTTAGATTACGATTTAATTGGAGAAATGACAAATGAATTTAGCTAA

Upstream 100 bases:

>100_bases
TTTTGACAGTAATAATACTATATTATATACTGAATTAGACAAATAAGTACAAAAATCGGGGTCACAAACCAAAGATTGGA
AAGGTGGATAAGTATTCAAA

Downstream 100 bases:

>100_bases
TAAGATAACAATAGCAAGAATCATACTGGCACCTGTTTTTATGGTGTTTTTACTGGTTACAGTTCCATATGGAGAATTAA
TAGCGTGGGCCATATTTATC

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGC
LSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSY
CAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRIT
DSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFM
QEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTE
GHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTNEFS

Sequences:

>Translated_444_residues
MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGC
LSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSY
CAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRIT
DSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFM
QEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTE
GHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTNEFS
>Mature_444_residues
MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGC
LSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSY
CAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRIT
DSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFM
QEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTE
GHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTNEFS

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=28.6307053941909, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=28.6307053941909, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=27.0509977827051, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=36.8303571428571, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=29.5045045045045, Blast_Score=194, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=440, Percent_Identity=28.4090909090909, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI71996771, Length=365, Percent_Identity=27.1232876712329, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21356207, Length=465, Percent_Identity=28.8172043010753, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=25.7777777777778, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_NATTJ (B2A3C0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917638.1
- ProteinModelPortal:   B2A3C0
- SMR:   B2A3C0
- GeneID:   6315468
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1467
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50388; Mature: 50388

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHI
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCCCHHHHHHHHH
LEVAQLKETGKLKVLIVAGCLSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNY
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCH
ISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSYCAIPLIRGGYRSRTIEDIKI
HHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEE
EANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRIT
CHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHH
DSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTS
HHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEE
LIVGFPGETDEKYQNLISFMQEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEA
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
WEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DFINVEIVQALDYDLIGEMTNEFS
CEEEEEHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHI
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCCCHHHHHHHHH
LEVAQLKETGKLKVLIVAGCLSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNY
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCH
ISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSYCAIPLIRGGYRSRTIEDIKI
HHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEE
EANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRIT
CHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHH
DSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTS
HHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEE
LIVGFPGETDEKYQNLISFMQEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEA
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
WEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DFINVEIVQALDYDLIGEMTNEFS
CEEEEEHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA