Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 188586085

GI number: 188586085

Start: 1528291

End: 1529178

Strand: Direct

Name: dapA

Synonym: Nther_1459

Alternate gene names: 188586085

Gene position: 1528291-1529178 (Clockwise)

Preceding gene: 188586084

Following gene: 188586086

Centisome position: 48.28

GC content: 36.26

Gene sequence:

>888_bases
TTGAGAGGGTTTGGTGAGTTAATAACTGCCATGATCACACCCTTTAACAAGCAAGGTGAACTGGCAACCGAAAATTGTGT
AAAATTAGCCAAAAAACTCGTGAATGAACAAAACTCAGATGCCTTAGTTTTATCTGGAACTACGGGGGAATCACCTACAT
TAAGTACAGAAGAAAAGTTGACTTTGTTTCAACTTATAACAGAAAAGGTAGGCGGAAACGCTAACATTATAGCAGGTACC
GGAAGTTACAGTACGAAAGAAACCTTGGAGTTGACCAAAAAGGCTGAAGAAGTAGGAGTCCATGGTATAATGCTGGTCAC
TCCATACTATAATAAACCTACTCAAGGAGATTTATATAAACATTTCAAGACAATAGCCCAAAAGACTTCTCTGCCGATTA
TGTTGTATAATGTCCCTAAGCGTACTGGCGTAGATATGTCTCAAGATACAATCTTAAAATTAGCAGAAATAGATAATATA
ATTGCTTTGAAAGAAGCCAGTGGAGACTTTAATAAAATTGCCAATGTAATCAGAGAAACTAGAGGAGAGTTTGATGTTTA
CAGTGGTGATGATATTAGCTTAATGCCTGTTTTAAGTCTTGGAGGGACTGGTGTAGTAAGTGTTGCTTCACATCTAATAG
GTAAAGAGCTAAAAGAAATAATAGATGCTTATCACGCAAATAATTCCAGAAAAGCTTTGGAATTGTTTCAAGAAATCTAT
CCTTTCATAACGGCATTATTAGTTAAGACAAATCCAATTCCCATAAAAGCTGCAGTCAACACTCATTTAATGCAAGTAGG
AAGTGTACGTCCACCTTTATATGATCTAAGTGAAAAGGAGCTAGAACAGCTATCAGAGGTGTATAAATGGTCAGTAGGTA
AATACTAA

Upstream 100 bases:

>100_bases
TCCTGTTTAATCCAGGGCAAGGATATTCAAAAAGCTTTGTTAGTTTTGCACGAACAATTTGGTCTGTCTCTTAAAAATTA
AACCAAAAGGGGGAGAAACA

Downstream 100 bases:

>100_bases
TCTTTATTTACCTAAAAATTTAATTATTCTTAAATCCCTTGAGATTGGTATTAAGAAGCCTTTATGGCTTCTTTTTCTTT
CTTGCTATGAGATAACAATT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRGFGELITAMITPFNKQGELATENCVKLAKKLVNEQNSDALVLSGTTGESPTLSTEEKLTLFQLITEKVGGNANIIAGT
GSYSTKETLELTKKAEEVGVHGIMLVTPYYNKPTQGDLYKHFKTIAQKTSLPIMLYNVPKRTGVDMSQDTILKLAEIDNI
IALKEASGDFNKIANVIRETRGEFDVYSGDDISLMPVLSLGGTGVVSVASHLIGKELKEIIDAYHANNSRKALELFQEIY
PFITALLVKTNPIPIKAAVNTHLMQVGSVRPPLYDLSEKELEQLSEVYKWSVGKY

Sequences:

>Translated_295_residues
MRGFGELITAMITPFNKQGELATENCVKLAKKLVNEQNSDALVLSGTTGESPTLSTEEKLTLFQLITEKVGGNANIIAGT
GSYSTKETLELTKKAEEVGVHGIMLVTPYYNKPTQGDLYKHFKTIAQKTSLPIMLYNVPKRTGVDMSQDTILKLAEIDNI
IALKEASGDFNKIANVIRETRGEFDVYSGDDISLMPVLSLGGTGVVSVASHLIGKELKEIIDAYHANNSRKALELFQEIY
PFITALLVKTNPIPIKAAVNTHLMQVGSVRPPLYDLSEKELEQLSEVYKWSVGKY
>Mature_295_residues
MRGFGELITAMITPFNKQGELATENCVKLAKKLVNEQNSDALVLSGTTGESPTLSTEEKLTLFQLITEKVGGNANIIAGT
GSYSTKETLELTKKAEEVGVHGIMLVTPYYNKPTQGDLYKHFKTIAQKTSLPIMLYNVPKRTGVDMSQDTILKLAEIDNI
IALKEASGDFNKIANVIRETRGEFDVYSGDDISLMPVLSLGGTGVVSVASHLIGKELKEIIDAYHANNSRKALELFQEIY
PFITALLVKTNPIPIKAAVNTHLMQVGSVRPPLYDLSEKELEQLSEVYKWSVGKY

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=249, Percent_Identity=28.9156626506024, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI13540533, Length=233, Percent_Identity=28.3261802575107, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=33.9041095890411, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI87082415, Length=299, Percent_Identity=27.7591973244147, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786463, Length=290, Percent_Identity=27.2413793103448, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789620, Length=288, Percent_Identity=28.4722222222222, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_NATTJ (B2A3B2)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917630.1
- ProteinModelPortal:   B2A3B2
- SMR:   B2A3B2
- GeneID:   6314936
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1459
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32413; Mature: 32413

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 164-164 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFGELITAMITPFNKQGELATENCVKLAKKLVNEQNSDALVLSGTTGESPTLSTEEKL
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHH
TLFQLITEKVGGNANIIAGTGSYSTKETLELTKKAEEVGVHGIMLVTPYYNKPTQGDLYK
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
HFKTIAQKTSLPIMLYNVPKRTGVDMSQDTILKLAEIDNIIALKEASGDFNKIANVIRET
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
RGEFDVYSGDDISLMPVLSLGGTGVVSVASHLIGKELKEIIDAYHANNSRKALELFQEIY
CCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PFITALLVKTNPIPIKAAVNTHLMQVGSVRPPLYDLSEKELEQLSEVYKWSVGKY
HHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRGFGELITAMITPFNKQGELATENCVKLAKKLVNEQNSDALVLSGTTGESPTLSTEEKL
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHH
TLFQLITEKVGGNANIIAGTGSYSTKETLELTKKAEEVGVHGIMLVTPYYNKPTQGDLYK
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
HFKTIAQKTSLPIMLYNVPKRTGVDMSQDTILKLAEIDNIIALKEASGDFNKIANVIRET
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
RGEFDVYSGDDISLMPVLSLGGTGVVSVASHLIGKELKEIIDAYHANNSRKALELFQEIY
CCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PFITALLVKTNPIPIKAAVNTHLMQVGSVRPPLYDLSEKELEQLSEVYKWSVGKY
HHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA