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The map label for this gene is dapG [H]

Identifier: 188586084

GI number: 188586084

Start: 1527033

End: 1528271

Strand: Direct

Name: dapG [H]

Synonym: Nther_1458

Alternate gene names: 188586084

Gene position: 1527033-1528271 (Clockwise)

Preceding gene: 188586083

Following gene: 188586085

Centisome position: 48.24

GC content: 36.24

Gene sequence:

>1239_bases
ATGACTGAAGATAAAATTATTGTCCAAAAATTTGGGGGTTCTTCTTTAAGGACACCAGATTTAAGACAACAAGCCGTTGA
ACACATTAAACAGCTAGTTCAGAATGATAAAAAGGTACTGGTAGTTGTAAGTGCCATTGGCAGAAGTCCTGACCCGTATG
CAACCGATACTTTGATAAATTTAATTAAAGAAGAAAATCCAGATCCAGACTTACGAGAACTAGACTTGGCTGTAAGTTGC
GGTGAAGTGCTATCTAGTGCTTTACTTGCCTCTAAATTACAGGCAGAGGGATATAAAGCGACAGCTTTGACAGGGCGACA
AGCTGGTATTATAACAGATAAAAACTTTACAGATGGTTCAATTAGAGAAATCAAAACTTCAATTATTCAAAAACTGTTTA
ACCAGAACAAAATTATTGTCGTCGCTGGTTTTCAAGGTGTAACTCCCAATGGTGAAATTATCACACTGGGAAGAGGTGGA
AGTGATACAACTGCAACGGCCTTTGCAGGGGAACTTGGCGCACAAAGGGTGGAAATATACAGTGATGTCGATGGCCTGAT
GACTGTTGATCCCAAAATGGTTCCTGATGCCCGACCAATTAAAGTTATCACCTTTTATGAAGTTCTACAAATGGCAAGAG
AAGGTGTCAAAGTGATTCATCCTAAAGCAGTTGAGTATGCCATGAAAAATTCAATACCAATTTTCATAAAAAATATTAAT
AAATATTATCAGGAAATAGGGACTAAAATAACGAGTTATATCTCAGACAAAGAAGAAGAACAGTCGAATAAAATTATCAC
AGGCATTGCCCACCTTCCCAATATTACTCAAGTCTCATTAGAGCTTGCCGAAAATACTTCACAAAGCGTGAAAGTTGATA
TTTTTAACGCTTTAGCTGAACAGGGTATCAGCCTGGATATGATTACTATTTATTACAACAATATAACTTTTACAGTTAAG
GGTGAAGACACTGAAAAAACCATTAAGCTATTGAATGAACGTGGTTTCGGTCAAATTAATCATCTAAGTGGTTGTGCCAA
AATTACACTATTTGGAACTGGGATGACAGGACTTCCTGGAGTAATGGCTAAAGCTCTAAGAATTTTAAATAAAGAAGAAA
TTGAAGTTCTACAAACAACTGATTCCAATATTACCATATCCTGTTTAATCCAGGGCAAGGATATTCAAAAAGCTTTGTTA
GTTTTGCACGAACAATTTGGTCTGTCTCTTAAAAATTAA

Upstream 100 bases:

>100_bases
CTGTGATCAGTAAAATGGACTTAATTATCCCGACAATACAACATGCATTAGAAGGTGAACAATTGCAACCCTTGTTATTG
GCAGGAGAGGAGTAATACTG

Downstream 100 bases:

>100_bases
ACCAAAAGGGGGAGAAACATTGAGAGGGTTTGGTGAGTTAATAACTGCCATGATCACACCCTTTAACAAGCAAGGTGAAC
TGGCAACCGAAAATTGTGTA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTEDKIIVQKFGGSSLRTPDLRQQAVEHIKQLVQNDKKVLVVVSAIGRSPDPYATDTLINLIKEENPDPDLRELDLAVSC
GEVLSSALLASKLQAEGYKATALTGRQAGIITDKNFTDGSIREIKTSIIQKLFNQNKIIVVAGFQGVTPNGEIITLGRGG
SDTTATAFAGELGAQRVEIYSDVDGLMTVDPKMVPDARPIKVITFYEVLQMAREGVKVIHPKAVEYAMKNSIPIFIKNIN
KYYQEIGTKITSYISDKEEEQSNKIITGIAHLPNITQVSLELAENTSQSVKVDIFNALAEQGISLDMITIYYNNITFTVK
GEDTEKTIKLLNERGFGQINHLSGCAKITLFGTGMTGLPGVMAKALRILNKEEIEVLQTTDSNITISCLIQGKDIQKALL
VLHEQFGLSLKN

Sequences:

>Translated_412_residues
MTEDKIIVQKFGGSSLRTPDLRQQAVEHIKQLVQNDKKVLVVVSAIGRSPDPYATDTLINLIKEENPDPDLRELDLAVSC
GEVLSSALLASKLQAEGYKATALTGRQAGIITDKNFTDGSIREIKTSIIQKLFNQNKIIVVAGFQGVTPNGEIITLGRGG
SDTTATAFAGELGAQRVEIYSDVDGLMTVDPKMVPDARPIKVITFYEVLQMAREGVKVIHPKAVEYAMKNSIPIFIKNIN
KYYQEIGTKITSYISDKEEEQSNKIITGIAHLPNITQVSLELAENTSQSVKVDIFNALAEQGISLDMITIYYNNITFTVK
GEDTEKTIKLLNERGFGQINHLSGCAKITLFGTGMTGLPGVMAKALRILNKEEIEVLQTTDSNITISCLIQGKDIQKALL
VLHEQFGLSLKN
>Mature_411_residues
TEDKIIVQKFGGSSLRTPDLRQQAVEHIKQLVQNDKKVLVVVSAIGRSPDPYATDTLINLIKEENPDPDLRELDLAVSCG
EVLSSALLASKLQAEGYKATALTGRQAGIITDKNFTDGSIREIKTSIIQKLFNQNKIIVVAGFQGVTPNGEIITLGRGGS
DTTATAFAGELGAQRVEIYSDVDGLMTVDPKMVPDARPIKVITFYEVLQMAREGVKVIHPKAVEYAMKNSIPIFIKNINK
YYQEIGTKITSYISDKEEEQSNKIITGIAHLPNITQVSLELAENTSQSVKVDIFNALAEQGISLDMITIYYNNITFTVKG
EDTEKTIKLLNERGFGQINHLSGCAKITLFGTGMTGLPGVMAKALRILNKEEIEVLQTTDSNITISCLIQGKDIQKALLV
LHEQFGLSLKN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=386, Percent_Identity=26.9430051813472, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790455, Length=277, Percent_Identity=28.5198555956679, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790376, Length=340, Percent_Identity=23.8235294117647, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320893, Length=364, Percent_Identity=25, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45128; Mature: 44996

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDKIIVQKFGGSSLRTPDLRQQAVEHIKQLVQNDKKVLVVVSAIGRSPDPYATDTLIN
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHH
LIKEENPDPDLRELDLAVSCGEVLSSALLASKLQAEGYKATALTGRQAGIITDKNFTDGS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCH
IREIKTSIIQKLFNQNKIIVVAGFQGVTPNGEIITLGRGGSDTTATAFAGELGAQRVEIY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHEEEE
SDVDGLMTVDPKMVPDARPIKVITFYEVLQMAREGVKVIHPKAVEYAMKNSIPIFIKNIN
CCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCEEECHHHHHHHHHCCCEEEEHHHH
KYYQEIGTKITSYISDKEEEQSNKIITGIAHLPNITQVSLELAENTSQSVKVDIFNALAE
HHHHHHHHHHHHHHCCCHHHHCCCEEEEHHHCCCCCEEEHHHHCCCCCCEEHHHHHHHHH
QGISLDMITIYYNNITFTVKGEDTEKTIKLLNERGFGQINHLSGCAKITLFGTGMTGLPG
CCCCEEEEEEEEECEEEEEECCCHHHHHHHHHHCCCCCCHHCCCEEEEEEEECCCCCCHH
VMAKALRILNKEEIEVLQTTDSNITISCLIQGKDIQKALLVLHEQFGLSLKN
HHHHHHHHHCHHHEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TEDKIIVQKFGGSSLRTPDLRQQAVEHIKQLVQNDKKVLVVVSAIGRSPDPYATDTLIN
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHH
LIKEENPDPDLRELDLAVSCGEVLSSALLASKLQAEGYKATALTGRQAGIITDKNFTDGS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCH
IREIKTSIIQKLFNQNKIIVVAGFQGVTPNGEIITLGRGGSDTTATAFAGELGAQRVEIY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHEEEE
SDVDGLMTVDPKMVPDARPIKVITFYEVLQMAREGVKVIHPKAVEYAMKNSIPIFIKNIN
CCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCEEECHHHHHHHHHCCCEEEEHHHH
KYYQEIGTKITSYISDKEEEQSNKIITGIAHLPNITQVSLELAENTSQSVKVDIFNALAE
HHHHHHHHHHHHHHCCCHHHHCCCEEEEHHHCCCCCEEEHHHHCCCCCCEEHHHHHHHHH
QGISLDMITIYYNNITFTVKGEDTEKTIKLLNERGFGQINHLSGCAKITLFGTGMTGLPG
CCCCEEEEEEEEECEEEEEECCCHHHHHHHHHHCCCCCCHHCCCEEEEEEEECCCCCCHH
VMAKALRILNKEEIEVLQTTDSNITISCLIQGKDIQKALLVLHEQFGLSLKN
HHHHHHHHHCHHHEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]