Click here to switch to the map view.

The map label for this gene is rnjA [H]

Identifier: 188586086

GI number: 188586086

Start: 1529352

End: 1531052

Strand: Direct

Name: rnjA [H]

Synonym: Nther_1460

Alternate gene names: 188586086

Gene position: 1529352-1531052 (Clockwise)

Preceding gene: 188586085

Following gene: 188586087

Centisome position: 48.31

GC content: 37.74

Gene sequence:

>1701_bases
TTGTCAGCAAGAAAACAGAATAATAGAAATGGTAATGGCTCCAGAAATTCATTACAAGTCATTCCCTTGGGCGGGGTCGG
TGAAATTGGAAAAAATATGTTTGTAATTAAGTACGGGAATGATATGATTGTCATTGACGCCGGATTAAAATTTCCCGATG
AAGATATGTTGGGAGTAGATTTCGTCATTCCTGACATGTCTTATCTGTTTGAACATAAGGACAACATTAAAGGAATTTTC
TTAACCCATGGGCATGAAGATCACATCGGGGCTTTACCGTATTTAATGAAAAAAATTGATGCACCAGTTTATGGTACTAA
ACTAACTTTAGGTTTGGCTGAACGTAAATTGGGAGAACAACCTATTCAAAAGTCGATTTCTTTAAAGAAGATATTACCTG
GCAAAACTATGCAATTAGGTCCATTTAAGATGGAATTTTTCTCAACTAACCACAGTATTCCAGATTCAATTGGCATAGTT
GTTCATACACCTGAAGGAACTATAGTCCATACAGGAGATTTTAAATTTGACCATACACCAGTTAGTGGTAAAGTAGCAGA
TTATAATAAACTTGCTGAACTGGGAAATAAGGGAGTACTATGCTTACTATCGGATAGTACCAATGCTGAAAGGCCGGGAT
ATACTGAAAGTGATAGAACAGTGGGAGAACGACTTGATGAAATCTTTCGCCAAGCTCCCAAAAGGATTATCCTGGCAACT
TTTGCCTCTAATATATACAGAATTCAGCAAGTTATTAACTCAGCCAAAAAGTATGGTAAAAAAGTCGGAGTCGTAGGTCG
CAGTATGATAAATGTGATTGAAGCGGCTTCAGAGCTAGGTTATCTTGAAAATGTAGACGATGTGGTGATTGATGATATAG
AAAGAGTCAATCAATTGCCTCTGGAAAAAACTGTGTTGCTAACTACGGGCAGTCAAGGAGAGCCCATGAGCGCATTAACC
AGGATTGCTACTGATGACCATCGAAAAGTCGAAATTATGCCCCATGATACTGTGGTGATAGCTGCAACACCTATTCCAGG
AAACGAAAAGTTAATTTCTAGAAGTATAGATAATTTGTTTAAACGAGGTGCCCATGTCATCTATCAATCTGTTTCCGGAG
TCCATGTATCCGGTCATGGAAGTCAGGAAGAATTGAAAATGATGCTGAACCTTGTTAAACCTAAGTATTTAATCCCTTTC
CATGGCGAGTACAGAATGCAGGTACATCATAAAAACGTAGCAGAAAAGTTAGGTATGCCATCAAAAAATGTATTTTTAGC
AGAAAATGGTGAAGTAATTGAATTTAACAGAAAACGAGGCAAAGTTAATGGCAAGGTTCCTGCAGGTAAAGTTTTAGTTG
ATGGTTTAGGTGTAGGTGATGTAGGTAATATCGTTCTAAGAGATCGACGACTGTTAAGCCAGGATGGTATCCTGATTGTA
GTGATTGGTGTAGATAAACAGAACAAAACATTAATTGCCGGCCCGGATATAGTTTCTAGAGGATTTGTATATGTCAGAGA
ATCAGAAGAGTTGTTAGAAGAGGCCCGTGAACATGTTGTTAAAGCTGTAAACAAGATGTCAAAAAGTAAAATGAATGAGT
GGCAGGCAATTAAGAGTTCTGTCAAAGATGTTTTAGGTCAATATCTATGGGAAAAAACCCGGAGACGTCCCATGATTCTT
CCAATAATTATGGAAGTTTAA

Upstream 100 bases:

>100_bases
TTTCTTTCTTGCTATGAGATAACAATTAAGGTATAATATTACTTAATGGTAAGGGAACGGGTATACAGCTATACACTCAA
AAAAACAGGGAGGTGTTTGA

Downstream 100 bases:

>100_bases
TCTATTAAACCCTTACCGAATTCAAATATCTATAAGCTATAAATTTTAGTTTATTAACCTCCAACAAAGTTGGAGGTTTT
TTTATTTTTTAATCAGTATA

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSARKQNNRNGNGSRNSLQVIPLGGVGEIGKNMFVIKYGNDMIVIDAGLKFPDEDMLGVDFVIPDMSYLFEHKDNIKGIF
LTHGHEDHIGALPYLMKKIDAPVYGTKLTLGLAERKLGEQPIQKSISLKKILPGKTMQLGPFKMEFFSTNHSIPDSIGIV
VHTPEGTIVHTGDFKFDHTPVSGKVADYNKLAELGNKGVLCLLSDSTNAERPGYTESDRTVGERLDEIFRQAPKRIILAT
FASNIYRIQQVINSAKKYGKKVGVVGRSMINVIEAASELGYLENVDDVVIDDIERVNQLPLEKTVLLTTGSQGEPMSALT
RIATDDHRKVEIMPHDTVVIAATPIPGNEKLISRSIDNLFKRGAHVIYQSVSGVHVSGHGSQEELKMMLNLVKPKYLIPF
HGEYRMQVHHKNVAEKLGMPSKNVFLAENGEVIEFNRKRGKVNGKVPAGKVLVDGLGVGDVGNIVLRDRRLLSQDGILIV
VIGVDKQNKTLIAGPDIVSRGFVYVRESEELLEEAREHVVKAVNKMSKSKMNEWQAIKSSVKDVLGQYLWEKTRRRPMIL
PIIMEV

Sequences:

>Translated_566_residues
MSARKQNNRNGNGSRNSLQVIPLGGVGEIGKNMFVIKYGNDMIVIDAGLKFPDEDMLGVDFVIPDMSYLFEHKDNIKGIF
LTHGHEDHIGALPYLMKKIDAPVYGTKLTLGLAERKLGEQPIQKSISLKKILPGKTMQLGPFKMEFFSTNHSIPDSIGIV
VHTPEGTIVHTGDFKFDHTPVSGKVADYNKLAELGNKGVLCLLSDSTNAERPGYTESDRTVGERLDEIFRQAPKRIILAT
FASNIYRIQQVINSAKKYGKKVGVVGRSMINVIEAASELGYLENVDDVVIDDIERVNQLPLEKTVLLTTGSQGEPMSALT
RIATDDHRKVEIMPHDTVVIAATPIPGNEKLISRSIDNLFKRGAHVIYQSVSGVHVSGHGSQEELKMMLNLVKPKYLIPF
HGEYRMQVHHKNVAEKLGMPSKNVFLAENGEVIEFNRKRGKVNGKVPAGKVLVDGLGVGDVGNIVLRDRRLLSQDGILIV
VIGVDKQNKTLIAGPDIVSRGFVYVRESEELLEEAREHVVKAVNKMSKSKMNEWQAIKSSVKDVLGQYLWEKTRRRPMIL
PIIMEV
>Mature_565_residues
SARKQNNRNGNGSRNSLQVIPLGGVGEIGKNMFVIKYGNDMIVIDAGLKFPDEDMLGVDFVIPDMSYLFEHKDNIKGIFL
THGHEDHIGALPYLMKKIDAPVYGTKLTLGLAERKLGEQPIQKSISLKKILPGKTMQLGPFKMEFFSTNHSIPDSIGIVV
HTPEGTIVHTGDFKFDHTPVSGKVADYNKLAELGNKGVLCLLSDSTNAERPGYTESDRTVGERLDEIFRQAPKRIILATF
ASNIYRIQQVINSAKKYGKKVGVVGRSMINVIEAASELGYLENVDDVVIDDIERVNQLPLEKTVLLTTGSQGEPMSALTR
IATDDHRKVEIMPHDTVVIAATPIPGNEKLISRSIDNLFKRGAHVIYQSVSGVHVSGHGSQEELKMMLNLVKPKYLIPFH
GEYRMQVHHKNVAEKLGMPSKNVFLAENGEVIEFNRKRGKVNGKVPAGKVLVDGLGVGDVGNIVLRDRRLLSQDGILIVV
IGVDKQNKTLIAGPDIVSRGFVYVRESEELLEEAREHVVKAVNKMSKSKMNEWQAIKSSVKDVLGQYLWEKTRRRPMILP
IIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62821; Mature: 62690

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARKQNNRNGNGSRNSLQVIPLGGVGEIGKNMFVIKYGNDMIVIDAGLKFPDEDMLGVD
CCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCEE
FVIPDMSYLFEHKDNIKGIFLTHGHEDHIGALPYLMKKIDAPVYGTKLTLGLAERKLGEQ
EECCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHCCHH
PIQKSISLKKILPGKTMQLGPFKMEFFSTNHSIPDSIGIVVHTPEGTIVHTGDFKFDHTP
HHHHCCCHHHCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCCCEEECCCCEECCCC
VSGKVADYNKLAELGNKGVLCLLSDSTNAERPGYTESDRTVGERLDEIFRQAPKRIILAT
CCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEHHH
FASNIYRIQQVINSAKKYGKKVGVVGRSMINVIEAASELGYLENVDDVVIDDIERVNQLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
LEKTVLLTTGSQGEPMSALTRIATDDHRKVEIMPHDTVVIAATPIPGNEKLISRSIDNLF
CCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHH
KRGAHVIYQSVSGVHVSGHGSQEELKMMLNLVKPKYLIPFHGEYRMQVHHKNVAEKLGMP
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHCCC
SKNVFLAENGEVIEFNRKRGKVNGKVPAGKVLVDGLGVGDVGNIVLRDRRLLSQDGILIV
CCCEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHCCCCEEEE
VIGVDKQNKTLIAGPDIVSRGFVYVRESEELLEEAREHVVKAVNKMSKSKMNEWQAIKSS
EEECCCCCCEEEECCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVLGQYLWEKTRRRPMILPIIMEV
HHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SARKQNNRNGNGSRNSLQVIPLGGVGEIGKNMFVIKYGNDMIVIDAGLKFPDEDMLGVD
CCCCCCCCCCCCCCCEEEEEEECCCHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCEE
FVIPDMSYLFEHKDNIKGIFLTHGHEDHIGALPYLMKKIDAPVYGTKLTLGLAERKLGEQ
EECCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHCCHH
PIQKSISLKKILPGKTMQLGPFKMEFFSTNHSIPDSIGIVVHTPEGTIVHTGDFKFDHTP
HHHHCCCHHHCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCCCEEECCCCEECCCC
VSGKVADYNKLAELGNKGVLCLLSDSTNAERPGYTESDRTVGERLDEIFRQAPKRIILAT
CCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEHHH
FASNIYRIQQVINSAKKYGKKVGVVGRSMINVIEAASELGYLENVDDVVIDDIERVNQLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
LEKTVLLTTGSQGEPMSALTRIATDDHRKVEIMPHDTVVIAATPIPGNEKLISRSIDNLF
CCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHH
KRGAHVIYQSVSGVHVSGHGSQEELKMMLNLVKPKYLIPFHGEYRMQVHHKNVAEKLGMP
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHCCC
SKNVFLAENGEVIEFNRKRGKVNGKVPAGKVLVDGLGVGDVGNIVLRDRRLLSQDGILIV
CCCEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHCCCCEEEE
VIGVDKQNKTLIAGPDIVSRGFVYVRESEELLEEAREHVVKAVNKMSKSKMNEWQAIKSS
EEECCCCCCEEEECCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVLGQYLWEKTRRRPMILPIIMEV
HHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]