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The map label for this gene is murF [H]

Identifier: 188585926

GI number: 188585926

Start: 1372400

End: 1373728

Strand: Direct

Name: murF [H]

Synonym: Nther_1300

Alternate gene names: 188585926

Gene position: 1372400-1373728 (Clockwise)

Preceding gene: 188585925

Following gene: 188585927

Centisome position: 43.35

GC content: 38.15

Gene sequence:

>1329_bases
ATGGGTGACCCTGATACTGTCATTACTGGCTTTAGTGTGGATTCACGTTATGTCAAGCAGGGCGATATGTTTGTATGCTT
ACCGGGAGATAAAACCGATGGTCATAATTTTATAGACGAAGCAATTGATAATGGTGCCCGAGGATGTCTAGTAAATCGTG
ATATTGAACTGTCTCGACGTCATTCAGTCCATCCACCCTTGGTAATAAAAGTCAATGATACGTTGATGGCCCTGCAAGAA
ATAGCCAGAAAGCATCGACAAAAATTTGGCGTTTCTGTGGTAGCTGTTACTGGTAGTATTGGTAAAACTACCACCAAAGA
TATGATCGCCAGTGTTTTAGCTGAAAAGTACTCTATTTTAAAAACCCACGGTAATTTGAATAGTGAAATTGGACTCCCTT
TGATGTTATTGGAATTAACTCAACATCATCAAGTTGCTGTACTTGAAATGGGAATGAACAATCAAGGAGAAATAGCCAGG
TTATGTGAGTTGGCAAGACCTAATGTAGGTGTTATCACAAGGATTGCCGAGTCTCATATAGAGAAGCTGGGCAGTTTAGA
AAACATTGCAGAAGCTAAAGGTGAACTGTTGGAGAATTTATTGCCTGGTGGGACTGCTATTTTGAATTGCGATGATAACT
GGGTTAGACGACTGAGGTCTAAAGTTCCAGGTGAAGTTTTATACTATGGTTTAACAGATGGAGATTTATCGGCTTATAAT
GTCAGAGTCGACAAGGACAAAACGAATGCGAAAATTATGAGTAATGATAAAAGTGAGCAGCTAAACTTACCTTTAATTGG
AAAGCATAACTTGAGTAATGCTTTGGCGGCAATTGCTGTTGGTAGAGTCTTTGGACTCGATTTGACTCAATCTATCCGTG
CACTTGAAGGTTTTACTGGTACATCAATGAGGAATCAAATAAAGTATCATAAAGACATGATAATTATTGATGATACTTAT
AATGCCAATGTACAATCTACCTTGGCAGCCTTTGATGTATTAACAAATTTACCACTCAACCGTAGAATAGCCGTTTTAGG
AGATATGTTCGAATTAGGTGATTATACAGAAAAAGCTCACAAAACTGTGGGTGAACAGGCTGTAAACAGGGGATTTGATT
ATTTGATTACAGTTGGTGAACTATCAAAAACCACTCAAGAAGCAGCTGATAAGTTGGGAATGCCTGAGCATCAAAACATT
CATGTTGCTGATCGTGAACAGGCTATTCAAAGGATATTACAGATAGTTAAATCTGGAGATGGAATACTTGTTAAGGGTTC
TAGAGGTATGGCTATGGAAAAGATAGTAAATTCGTTGTTAAAAACTTAG

Upstream 100 bases:

>100_bases
GATGATCGTGAGATAGCGAGAGAAAAATTGAAAAAAATGGGGTATTAGTATGGTTTGGACCGGACATGAACTTACTAGTA
ATTGTCGGGGAAATATAATT

Downstream 100 bases:

>100_bases
GAGGATCGAATATGGAAAAAATATACACATCAATATTAATAGCTTTTGTAATCAGTTTAGTATTATCACCCATTTTTATA
CCGTTATTGAGGAAACTTAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MGDPDTVITGFSVDSRYVKQGDMFVCLPGDKTDGHNFIDEAIDNGARGCLVNRDIELSRRHSVHPPLVIKVNDTLMALQE
IARKHRQKFGVSVVAVTGSIGKTTTKDMIASVLAEKYSILKTHGNLNSEIGLPLMLLELTQHHQVAVLEMGMNNQGEIAR
LCELARPNVGVITRIAESHIEKLGSLENIAEAKGELLENLLPGGTAILNCDDNWVRRLRSKVPGEVLYYGLTDGDLSAYN
VRVDKDKTNAKIMSNDKSEQLNLPLIGKHNLSNALAAIAVGRVFGLDLTQSIRALEGFTGTSMRNQIKYHKDMIIIDDTY
NANVQSTLAAFDVLTNLPLNRRIAVLGDMFELGDYTEKAHKTVGEQAVNRGFDYLITVGELSKTTQEAADKLGMPEHQNI
HVADREQAIQRILQIVKSGDGILVKGSRGMAMEKIVNSLLKT

Sequences:

>Translated_442_residues
MGDPDTVITGFSVDSRYVKQGDMFVCLPGDKTDGHNFIDEAIDNGARGCLVNRDIELSRRHSVHPPLVIKVNDTLMALQE
IARKHRQKFGVSVVAVTGSIGKTTTKDMIASVLAEKYSILKTHGNLNSEIGLPLMLLELTQHHQVAVLEMGMNNQGEIAR
LCELARPNVGVITRIAESHIEKLGSLENIAEAKGELLENLLPGGTAILNCDDNWVRRLRSKVPGEVLYYGLTDGDLSAYN
VRVDKDKTNAKIMSNDKSEQLNLPLIGKHNLSNALAAIAVGRVFGLDLTQSIRALEGFTGTSMRNQIKYHKDMIIIDDTY
NANVQSTLAAFDVLTNLPLNRRIAVLGDMFELGDYTEKAHKTVGEQAVNRGFDYLITVGELSKTTQEAADKLGMPEHQNI
HVADREQAIQRILQIVKSGDGILVKGSRGMAMEKIVNSLLKT
>Mature_441_residues
GDPDTVITGFSVDSRYVKQGDMFVCLPGDKTDGHNFIDEAIDNGARGCLVNRDIELSRRHSVHPPLVIKVNDTLMALQEI
ARKHRQKFGVSVVAVTGSIGKTTTKDMIASVLAEKYSILKTHGNLNSEIGLPLMLLELTQHHQVAVLEMGMNNQGEIARL
CELARPNVGVITRIAESHIEKLGSLENIAEAKGELLENLLPGGTAILNCDDNWVRRLRSKVPGEVLYYGLTDGDLSAYNV
RVDKDKTNAKIMSNDKSEQLNLPLIGKHNLSNALAAIAVGRVFGLDLTQSIRALEGFTGTSMRNQIKYHKDMIIIDDTYN
ANVQSTLAAFDVLTNLPLNRRIAVLGDMFELGDYTEKAHKTVGEQAVNRGFDYLITVGELSKTTQEAADKLGMPEHQNIH
VADREQAIQRILQIVKSGDGILVKGSRGMAMEKIVNSLLKT

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=443, Percent_Identity=37.2460496613996, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48530; Mature: 48399

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDPDTVITGFSVDSRYVKQGDMFVCLPGDKTDGHNFIDEAIDNGARGCLVNRDIELSRR
CCCCCCEEEECCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHC
HSVHPPLVIKVNDTLMALQEIARKHRQKFGVSVVAVTGSIGKTTTKDMIASVLAEKYSIL
CCCCCCEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
KTHGNLNSEIGLPLMLLELTQHHQVAVLEMGMNNQGEIARLCELARPNVGVITRIAESHI
HHCCCCCCCCCCHHHEEHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
EKLGSLENIAEAKGELLENLLPGGTAILNCDDNWVRRLRSKVPGEVLYYGLTDGDLSAYN
HHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCEEEE
VRVDKDKTNAKIMSNDKSEQLNLPLIGKHNLSNALAAIAVGRVFGLDLTQSIRALEGFTG
EEECCCCCCCEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TSMRNQIKYHKDMIIIDDTYNANVQSTLAAFDVLTNLPLNRRIAVLGDMFELGDYTEKAH
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHH
KTVGEQAVNRGFDYLITVGELSKTTQEAADKLGMPEHQNIHVADREQAIQRILQIVKSGD
HHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHCCC
GILVKGSRGMAMEKIVNSLLKT
CEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GDPDTVITGFSVDSRYVKQGDMFVCLPGDKTDGHNFIDEAIDNGARGCLVNRDIELSRR
CCCCCEEEECCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHC
HSVHPPLVIKVNDTLMALQEIARKHRQKFGVSVVAVTGSIGKTTTKDMIASVLAEKYSIL
CCCCCCEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
KTHGNLNSEIGLPLMLLELTQHHQVAVLEMGMNNQGEIARLCELARPNVGVITRIAESHI
HHCCCCCCCCCCHHHEEHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
EKLGSLENIAEAKGELLENLLPGGTAILNCDDNWVRRLRSKVPGEVLYYGLTDGDLSAYN
HHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEECCCCCCEEEE
VRVDKDKTNAKIMSNDKSEQLNLPLIGKHNLSNALAAIAVGRVFGLDLTQSIRALEGFTG
EEECCCCCCCEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TSMRNQIKYHKDMIIIDDTYNANVQSTLAAFDVLTNLPLNRRIAVLGDMFELGDYTEKAH
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHH
KTVGEQAVNRGFDYLITVGELSKTTQEAADKLGMPEHQNIHVADREQAIQRILQIVKSGD
HHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHCCC
GILVKGSRGMAMEKIVNSLLKT
CEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]