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The map label for this gene is murE [H]

Identifier: 188585925

GI number: 188585925

Start: 1370743

End: 1372347

Strand: Direct

Name: murE [H]

Synonym: Nther_1299

Alternate gene names: 188585925

Gene position: 1370743-1372347 (Clockwise)

Preceding gene: 188585924

Following gene: 188585926

Centisome position: 43.3

GC content: 36.01

Gene sequence:

>1605_bases
ATGAAGAGTAAGACATTAAATAACCTTCATAGATTAACAAGGTTAAACTATGAATCAAAAACAGAAATTAATAACAGGGA
GAGGAGTGAAATTATCAAATTAGCCGATTTAGTAAAACCACTACTAAAAGTTAAAGTATTTGGCAAATTAGACGGTGAAA
TTTCCGGCCTGTCATACGACTCCCGATTTACTAAACCAGGCCAATTGTTCACATGTATCAAAGGAACAAAAGATGATGGA
CATAAGTATTTAGAAGATGTATTATCTAAGGGTGTTAGTGCCGTAGTGGCTCAATACGACTGCTTGTCATCAGAACAGAA
AAAGTTATTAACCCAGATAGATATTCCGGTAATACTGGTGACAGACTCTAGAAAAGCTTTTGCCATCTTGGCGAGTAAAT
ACTACAACTATCCTGGTGATAGTTTGCGAGTCACTGGTGTGACAGGAACTAAAGGGAAAACCACTACTGTAAATCTAATA
GATAAGCTACTTTCTGAAGGCAAAGAAGTGAAAACTGGCATGATTGGAACAATCAATGCAAGAATAGATGACCAAGAGGA
AAAGCAGCCAACTACAACCCCAGGTCCTTTAGAGTTAAATGAACTATTAGGGAGAATTGAAAACTCAGGGGCTAGTCATG
TAACCCTAGAGGTTAGTTCTCATGCCATATCTCAATTAAGAATTGCCGGTGTTGATTTAGATATAGCAGTGTATACCAAT
TTGGCACCTGAACATTTAGACTACCATACTGATATGGATGAATATAAGAGAACTAAAGGGCGTATATTTTCTTTTCAAGG
CCTTAAGCCTGAAAAAAATGATAAACCTAAACTAGCTGTTATTAACTTAGATGATAAATATGCCAGCTACATGTTAGAAC
AACCTGCCGTACAAGTTTTGACACACGGCTTTGATGAAAATGCACATGTAAAAGGTAAAATTATAAACACAGCTGAAACG
TTTACGGAGCTAAAAATAACATCTCCTTGGGGAAATTTCACTGTTAAAACTCCTTTAATAGGAAAATTCAATTGTTACAA
TTTGCTTGCAGCAATTACCGTAGCTTTGTTTGAGGGTATTCCTGCTTCCAAAATTCAAACAATCCTGGAACAACCTATTA
ATATTCCAGGAAGATTTGAAAGAATTGACTTAGGTCAAGAATTTACAGTAGTAGTAGATTTTGCTCATACTACTGATTCA
TTGCAGGAAGTACTACAATCCTTACGTCAAATGGTATCGGGAAAAATTGGAGTTGTATTTGGCTGTGGAGGAGAAAGGGA
TAAAAGTAAAAGACCTAAAATGGGTCGTGCGGCTTCTGATTTGGGTGATAAGATATTTATCACTTCTGATAATCCTAGAG
CAGAAGATCCCCAAAAAATTGTGCAAGATATATTATCAGGTTGTGATGTTAACAATAATGAAAAAATACAAATTGAATTA
GATAGGGCACAGGCCATAGAAAAATCTCTAAATTGGGCTAGAGAAGGAGATGCTGTCTTGATAGCAGGTAAAGGACATGA
AGCTACTCAGGAGATAAACGGAAAAGTTATTAACTTTGATGATCGTGAGATAGCGAGAGAAAAATTGAAAAAAATGGGGT
ATTAG

Upstream 100 bases:

>100_bases
GAAGGCAGTGGTATTGCTGTTACACAAAATCCGGAAAAGGGGACGAAAGTAGATAAAGGTACATCCGTAACTGTCCAGTT
CGAACCTCCCGATGAATTCA

Downstream 100 bases:

>100_bases
TATGGTTTGGACCGGACATGAACTTACTAGTAATTGTCGGGGAAATATAATTATGGGTGACCCTGATACTGTCATTACTG
GCTTTAGTGTGGATTCACGT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKSKTLNNLHRLTRLNYESKTEINNRERSEIIKLADLVKPLLKVKVFGKLDGEISGLSYDSRFTKPGQLFTCIKGTKDDG
HKYLEDVLSKGVSAVVAQYDCLSSEQKKLLTQIDIPVILVTDSRKAFAILASKYYNYPGDSLRVTGVTGTKGKTTTVNLI
DKLLSEGKEVKTGMIGTINARIDDQEEKQPTTTPGPLELNELLGRIENSGASHVTLEVSSHAISQLRIAGVDLDIAVYTN
LAPEHLDYHTDMDEYKRTKGRIFSFQGLKPEKNDKPKLAVINLDDKYASYMLEQPAVQVLTHGFDENAHVKGKIINTAET
FTELKITSPWGNFTVKTPLIGKFNCYNLLAAITVALFEGIPASKIQTILEQPINIPGRFERIDLGQEFTVVVDFAHTTDS
LQEVLQSLRQMVSGKIGVVFGCGGERDKSKRPKMGRAASDLGDKIFITSDNPRAEDPQKIVQDILSGCDVNNNEKIQIEL
DRAQAIEKSLNWAREGDAVLIAGKGHEATQEINGKVINFDDREIAREKLKKMGY

Sequences:

>Translated_534_residues
MKSKTLNNLHRLTRLNYESKTEINNRERSEIIKLADLVKPLLKVKVFGKLDGEISGLSYDSRFTKPGQLFTCIKGTKDDG
HKYLEDVLSKGVSAVVAQYDCLSSEQKKLLTQIDIPVILVTDSRKAFAILASKYYNYPGDSLRVTGVTGTKGKTTTVNLI
DKLLSEGKEVKTGMIGTINARIDDQEEKQPTTTPGPLELNELLGRIENSGASHVTLEVSSHAISQLRIAGVDLDIAVYTN
LAPEHLDYHTDMDEYKRTKGRIFSFQGLKPEKNDKPKLAVINLDDKYASYMLEQPAVQVLTHGFDENAHVKGKIINTAET
FTELKITSPWGNFTVKTPLIGKFNCYNLLAAITVALFEGIPASKIQTILEQPINIPGRFERIDLGQEFTVVVDFAHTTDS
LQEVLQSLRQMVSGKIGVVFGCGGERDKSKRPKMGRAASDLGDKIFITSDNPRAEDPQKIVQDILSGCDVNNNEKIQIEL
DRAQAIEKSLNWAREGDAVLIAGKGHEATQEINGKVINFDDREIAREKLKKMGY
>Mature_534_residues
MKSKTLNNLHRLTRLNYESKTEINNRERSEIIKLADLVKPLLKVKVFGKLDGEISGLSYDSRFTKPGQLFTCIKGTKDDG
HKYLEDVLSKGVSAVVAQYDCLSSEQKKLLTQIDIPVILVTDSRKAFAILASKYYNYPGDSLRVTGVTGTKGKTTTVNLI
DKLLSEGKEVKTGMIGTINARIDDQEEKQPTTTPGPLELNELLGRIENSGASHVTLEVSSHAISQLRIAGVDLDIAVYTN
LAPEHLDYHTDMDEYKRTKGRIFSFQGLKPEKNDKPKLAVINLDDKYASYMLEQPAVQVLTHGFDENAHVKGKIINTAET
FTELKITSPWGNFTVKTPLIGKFNCYNLLAAITVALFEGIPASKIQTILEQPINIPGRFERIDLGQEFTVVVDFAHTTDS
LQEVLQSLRQMVSGKIGVVFGCGGERDKSKRPKMGRAASDLGDKIFITSDNPRAEDPQKIVQDILSGCDVNNNEKIQIEL
DRAQAIEKSLNWAREGDAVLIAGKGHEATQEINGKVINFDDREIAREKLKKMGY

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=32.76955602537, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 59283; Mature: 59283

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKTLNNLHRLTRLNYESKTEINNRERSEIIKLADLVKPLLKVKVFGKLDGEISGLSYD
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
SRFTKPGQLFTCIKGTKDDGHKYLEDVLSKGVSAVVAQYDCLSSEQKKLLTQIDIPVILV
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEE
TDSRKAFAILASKYYNYPGDSLRVTGVTGTKGKTTTVNLIDKLLSEGKEVKTGMIGTINA
ECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEC
RIDDQEEKQPTTTPGPLELNELLGRIENSGASHVTLEVSSHAISQLRIAGVDLDIAVYTN
CCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHEEEECCEEEEEEEEC
LAPEHLDYHTDMDEYKRTKGRIFSFQGLKPEKNDKPKLAVINLDDKYASYMLEQPAVQVL
CCHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
THGFDENAHVKGKIINTAETFTELKITSPWGNFTVKTPLIGKFNCYNLLAAITVALFEGI
HCCCCCCCEEEEEEEECHHCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCC
PASKIQTILEQPINIPGRFERIDLGQEFTVVVDFAHTTDSLQEVLQSLRQMVSGKIGVVF
CHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
GCGGERDKSKRPKMGRAASDLGDKIFITSDNPRAEDPQKIVQDILSGCDVNNNEKIQIEL
CCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
DRAQAIEKSLNWAREGDAVLIAGKGHEATQEINGKVINFDDREIAREKLKKMGY
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKTLNNLHRLTRLNYESKTEINNRERSEIIKLADLVKPLLKVKVFGKLDGEISGLSYD
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
SRFTKPGQLFTCIKGTKDDGHKYLEDVLSKGVSAVVAQYDCLSSEQKKLLTQIDIPVILV
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEE
TDSRKAFAILASKYYNYPGDSLRVTGVTGTKGKTTTVNLIDKLLSEGKEVKTGMIGTINA
ECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEC
RIDDQEEKQPTTTPGPLELNELLGRIENSGASHVTLEVSSHAISQLRIAGVDLDIAVYTN
CCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHEEEECCEEEEEEEEC
LAPEHLDYHTDMDEYKRTKGRIFSFQGLKPEKNDKPKLAVINLDDKYASYMLEQPAVQVL
CCHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
THGFDENAHVKGKIINTAETFTELKITSPWGNFTVKTPLIGKFNCYNLLAAITVALFEGI
HCCCCCCCEEEEEEEECHHCEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCC
PASKIQTILEQPINIPGRFERIDLGQEFTVVVDFAHTTDSLQEVLQSLRQMVSGKIGVVF
CHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
GCGGERDKSKRPKMGRAASDLGDKIFITSDNPRAEDPQKIVQDILSGCDVNNNEKIQIEL
CCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
DRAQAIEKSLNWAREGDAVLIAGKGHEATQEINGKVINFDDREIAREKLKKMGY
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA