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The map label for this gene is aspS

Identifier: 188585918

GI number: 188585918

Start: 1364050

End: 1365807

Strand: Direct

Name: aspS

Synonym: Nther_1292

Alternate gene names: 188585918

Gene position: 1364050-1365807 (Clockwise)

Preceding gene: 188585917

Following gene: 188585919

Centisome position: 43.09

GC content: 36.92

Gene sequence:

>1758_bases
ATGAAAAGAACACATTACTGTGGAGAATTGACCAGGGAATTAGTTGGGAAAAAAGTTGTCCTTAAGGGATGGGCTGACAA
TCGACGTGATCATGGAAAACTGATTTTTATCGACATGAGAGACAATTCGGGTTTAGTTCAGGTGGTATGTGATTTTGAAT
CTAATCCTGAAGCTTTAGAAGTAGCTGATTCGGTTAGATCCGAGTATGTTCTTGAAATCACCGGTACTGTTAGAGAAAGG
TCACCGGAAAATGTTAATCCAGATCTGAAGACAGGTGAAATAGAAGTAGACTGTGAGGATATTAATGTATTAAATACTTC
CAAAACACCTCCTTTCTTTATTAATGAAAATGTGGATGTGGATGAGAACATACGCTTAAAGTATCGATATTTAGATCTTA
GAAGACCATCAATGCAAAATAATATCTACTTAAGACATAAAATCACTAAACTTGTGAGAGACTTTTTAGATGAGAACGGT
TTTATTGAAGTTGAAACACCTATGCTGACTAAAAGTACTCCAGAAGGTGCAAGAGACTTTTTGGTTCCTAGCCGGTTACA
TGAGGGATCATTTTATGCGTTGCCTCAATCTCCCCAATTGTTTAAACAATTATTGATGGCTTCAGGAGTGGAAAAATATT
TCCAAATAGCTCGGTGTTTTAGAGATGAAGACTTAAGGGCTGATAGACAACCAGAATTTAGTCAAATCGATATTGAGATG
TCTTTTTTCGAACAAGAAGAGTTTATGAAATTAATGGAAAACATGGTATCAAAATTATTTAAAGAAGTATTGGGAATTGA
GTTAACTAAGCCATTCCCGCGAATAACATACCAGGAGGCTATGGACAGATATGGATCTGATAGCCCTGATTTAAGATACG
GTTTAGAATTACATGATGTATCTGATTTGGTGAAAGATGCAGAATTTAAAGTATTTAGAGAAACTGTGGCTAGCGAAGGT
CAAGTTAAAGGAATTGCCGTCCCTCAAGGACAAGAATTCAGTCGAAAAGAAATTGACGATTTAACGGAATTTGTTAAGGA
ATTCGGTGCGAAAGGGCTAGCATGGATGGTTTTAGAGGAAGAAGAAATAAAAAGTCCAATAGCTAAATTCTTTAGTGACG
AAGAATTAGACGGAATTATTGATCGAATGGGCGCCAATACAGGTGATTTACTGTTATTTGTAGCCGATGAACCCGAAATA
GTAGCAGATTCCTTATCAAAATTACGTGAGCATTTGGCTAATAAGTTAGATCTTATTCCTAGCAATGAATATCAACTTAC
ATGGGTAATTGATTTCCCCTTAATGGAATATGATAAAGATGAAGGAAGATATAAAGCATTGCATCATCCATTCACATCAC
CTTATGAAGATGACTTAGAAAAATATGAGAATGAACCAGAAAAGATACGCGCCAAAGCTTATGATCTAGTATTGAATGGA
GTAGAAATCGGTGGGGGGAGTATGAGAATCCATCAGAAAGAGTTACAGGAGAAAATGTTCGAATTTCTAGGAATCCCTCT
AGAAGAGGCTAAACAAAAGTTCGGTTTCTTGTTCGAAGCCTTTGAATATGGAACACCACCCCATGGCGGAATTGCTTTTG
GACTTGATCGATTAGTAATGTTGTTTACTGGCTCTCAAAGTATCAGAGATGTGATAGCATTTCCAAAAACTGCTAACGCA
AGTTGCCTAATGACTGAGGCTCCTGCAAAAGTTGATGAAAACCAGCTCAAAGAGTTGCACTTGAAAACCACGAAGTAA

Upstream 100 bases:

>100_bases
ATGGAAGCAGGCGAACAATTTCAAGTTCCTTTAAAGAGCTTTGAAGAAAGGATAAAAGAGATAGCTGGTTAAAACAAATT
AAGGATAGGGGGTTTGTAAT

Downstream 100 bases:

>100_bases
AATCGGATGTTTAATGATAATTGATTTTTGGGTCTAGATATGTTATTATTTCATTAAACAAAGCAATAGATAAGCAGATT
TGAAAGCGTCCCTACTGTGT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MKRTHYCGELTRELVGKKVVLKGWADNRRDHGKLIFIDMRDNSGLVQVVCDFESNPEALEVADSVRSEYVLEITGTVRER
SPENVNPDLKTGEIEVDCEDINVLNTSKTPPFFINENVDVDENIRLKYRYLDLRRPSMQNNIYLRHKITKLVRDFLDENG
FIEVETPMLTKSTPEGARDFLVPSRLHEGSFYALPQSPQLFKQLLMASGVEKYFQIARCFRDEDLRADRQPEFSQIDIEM
SFFEQEEFMKLMENMVSKLFKEVLGIELTKPFPRITYQEAMDRYGSDSPDLRYGLELHDVSDLVKDAEFKVFRETVASEG
QVKGIAVPQGQEFSRKEIDDLTEFVKEFGAKGLAWMVLEEEEIKSPIAKFFSDEELDGIIDRMGANTGDLLLFVADEPEI
VADSLSKLREHLANKLDLIPSNEYQLTWVIDFPLMEYDKDEGRYKALHHPFTSPYEDDLEKYENEPEKIRAKAYDLVLNG
VEIGGGSMRIHQKELQEKMFEFLGIPLEEAKQKFGFLFEAFEYGTPPHGGIAFGLDRLVMLFTGSQSIRDVIAFPKTANA
SCLMTEAPAKVDENQLKELHLKTTK

Sequences:

>Translated_585_residues
MKRTHYCGELTRELVGKKVVLKGWADNRRDHGKLIFIDMRDNSGLVQVVCDFESNPEALEVADSVRSEYVLEITGTVRER
SPENVNPDLKTGEIEVDCEDINVLNTSKTPPFFINENVDVDENIRLKYRYLDLRRPSMQNNIYLRHKITKLVRDFLDENG
FIEVETPMLTKSTPEGARDFLVPSRLHEGSFYALPQSPQLFKQLLMASGVEKYFQIARCFRDEDLRADRQPEFSQIDIEM
SFFEQEEFMKLMENMVSKLFKEVLGIELTKPFPRITYQEAMDRYGSDSPDLRYGLELHDVSDLVKDAEFKVFRETVASEG
QVKGIAVPQGQEFSRKEIDDLTEFVKEFGAKGLAWMVLEEEEIKSPIAKFFSDEELDGIIDRMGANTGDLLLFVADEPEI
VADSLSKLREHLANKLDLIPSNEYQLTWVIDFPLMEYDKDEGRYKALHHPFTSPYEDDLEKYENEPEKIRAKAYDLVLNG
VEIGGGSMRIHQKELQEKMFEFLGIPLEEAKQKFGFLFEAFEYGTPPHGGIAFGLDRLVMLFTGSQSIRDVIAFPKTANA
SCLMTEAPAKVDENQLKELHLKTTK
>Mature_585_residues
MKRTHYCGELTRELVGKKVVLKGWADNRRDHGKLIFIDMRDNSGLVQVVCDFESNPEALEVADSVRSEYVLEITGTVRER
SPENVNPDLKTGEIEVDCEDINVLNTSKTPPFFINENVDVDENIRLKYRYLDLRRPSMQNNIYLRHKITKLVRDFLDENG
FIEVETPMLTKSTPEGARDFLVPSRLHEGSFYALPQSPQLFKQLLMASGVEKYFQIARCFRDEDLRADRQPEFSQIDIEM
SFFEQEEFMKLMENMVSKLFKEVLGIELTKPFPRITYQEAMDRYGSDSPDLRYGLELHDVSDLVKDAEFKVFRETVASEG
QVKGIAVPQGQEFSRKEIDDLTEFVKEFGAKGLAWMVLEEEEIKSPIAKFFSDEELDGIIDRMGANTGDLLLFVADEPEI
VADSLSKLREHLANKLDLIPSNEYQLTWVIDFPLMEYDKDEGRYKALHHPFTSPYEDDLEKYENEPEKIRAKAYDLVLNG
VEIGGGSMRIHQKELQEKMFEFLGIPLEEAKQKFGFLFEAFEYGTPPHGGIAFGLDRLVMLFTGSQSIRDVIAFPKTANA
SCLMTEAPAKVDENQLKELHLKTTK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=40.9090909090909, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI194272210, Length=283, Percent_Identity=26.5017667844523, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI5031815, Length=283, Percent_Identity=26.5017667844523, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI45439306, Length=273, Percent_Identity=26.3736263736264, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4758762, Length=289, Percent_Identity=25.2595155709343, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226491502, Length=271, Percent_Identity=23.9852398523985, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=51.7826825127334, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1790571, Length=315, Percent_Identity=27.6190476190476, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789256, Length=306, Percent_Identity=26.797385620915, Blast_Score=103, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=594, Percent_Identity=37.3737373737374, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=306, Percent_Identity=22.8758169934641, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=315, Percent_Identity=22.8571428571429, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=306, Percent_Identity=22.8758169934641, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=27.2388059701493, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=285, Percent_Identity=24.2105263157895, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=35.3407290015848, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6320242, Length=265, Percent_Identity=26.0377358490566, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=25.7462686567164, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=155, Percent_Identity=29.0322580645161, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24584738, Length=596, Percent_Identity=37.751677852349, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=269, Percent_Identity=27.8810408921933, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640851, Length=395, Percent_Identity=24.8101265822785, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=395, Percent_Identity=24.8101265822785, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_NATTJ (B2A2G0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917463.1
- ProteinModelPortal:   B2A2G0
- SMR:   B2A2G0
- GeneID:   6315185
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1292
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67254; Mature: 67254

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTHYCGELTRELVGKKVVLKGWADNRRDHGKLIFIDMRDNSGLVQVVCDFESNPEALE
CCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHH
VADSVRSEYVLEITGTVRERSPENVNPDLKTGEIEVDCEDINVLNTSKTPPFFINENVDV
HHHHHCCCEEEEEECCHHCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCEEECCCCCC
DENIRLKYRYLDLRRPSMQNNIYLRHKITKLVRDFLDENGFIEVETPMLTKSTPEGARDF
CCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHC
LVPSRLHEGSFYALPQSPQLFKQLLMASGVEKYFQIARCFRDEDLRADRQPEFSQIDIEM
CCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEH
SFFEQEEFMKLMENMVSKLFKEVLGIELTKPFPRITYQEAMDRYGSDSPDLRYGLELHDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHHHH
SDLVKDAEFKVFRETVASEGQVKGIAVPQGQEFSRKEIDDLTEFVKEFGAKGLAWMVLEE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECH
EEIKSPIAKFFSDEELDGIIDRMGANTGDLLLFVADEPEIVADSLSKLREHLANKLDLIP
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
SNEYQLTWVIDFPLMEYDKDEGRYKALHHPFTSPYEDDLEKYENEPEKIRAKAYDLVLNG
CCCEEEEEEEECCCCCCCCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHEEEC
VEIGGGSMRIHQKELQEKMFEFLGIPLEEAKQKFGFLFEAFEYGTPPHGGIAFGLDRLVM
EEECCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LFTGSQSIRDVIAFPKTANASCLMTEAPAKVDENQLKELHLKTTK
HHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKRTHYCGELTRELVGKKVVLKGWADNRRDHGKLIFIDMRDNSGLVQVVCDFESNPEALE
CCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHH
VADSVRSEYVLEITGTVRERSPENVNPDLKTGEIEVDCEDINVLNTSKTPPFFINENVDV
HHHHHCCCEEEEEECCHHCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCEEECCCCCC
DENIRLKYRYLDLRRPSMQNNIYLRHKITKLVRDFLDENGFIEVETPMLTKSTPEGARDF
CCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHC
LVPSRLHEGSFYALPQSPQLFKQLLMASGVEKYFQIARCFRDEDLRADRQPEFSQIDIEM
CCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEH
SFFEQEEFMKLMENMVSKLFKEVLGIELTKPFPRITYQEAMDRYGSDSPDLRYGLELHDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHHHH
SDLVKDAEFKVFRETVASEGQVKGIAVPQGQEFSRKEIDDLTEFVKEFGAKGLAWMVLEE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECH
EEIKSPIAKFFSDEELDGIIDRMGANTGDLLLFVADEPEIVADSLSKLREHLANKLDLIP
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
SNEYQLTWVIDFPLMEYDKDEGRYKALHHPFTSPYEDDLEKYENEPEKIRAKAYDLVLNG
CCCEEEEEEEECCCCCCCCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHEEEC
VEIGGGSMRIHQKELQEKMFEFLGIPLEEAKQKFGFLFEAFEYGTPPHGGIAFGLDRLVM
EEECCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LFTGSQSIRDVIAFPKTANASCLMTEAPAKVDENQLKELHLKTTK
HHCCCHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA