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The map label for this gene is hisS

Identifier: 188585917

GI number: 188585917

Start: 1362756

End: 1364021

Strand: Direct

Name: hisS

Synonym: Nther_1291

Alternate gene names: 188585917

Gene position: 1362756-1364021 (Clockwise)

Preceding gene: 188585916

Following gene: 188585918

Centisome position: 43.05

GC content: 37.99

Gene sequence:

>1266_bases
ATGTTGACGAAAGCTCCAAGGGGAACTAAAGATATTTTGCCTCAAGAGAGTAGCAAGTGGCATGCTTTAGAGGAGACGGC
AAAGAAAATCTCAAACTTGTACGGTTTCAATGAAGTAAGGTTTCCAATTTTTGAACATACTGAACTATTTACTCGCAGTG
TTGGAGAGACCTCTGATATAGTTAGTAAAGAAATGTATACTTTTACCGATAGGGGAGATAGAAGTCTTACATTAAGGCCA
GAGGGTACAGCTCCAGCAGCCAGACTGTACGTAGAACATAAATTGTTTAACGAGCCTCAACCTGTAAAGTATTATTATAT
TGGCCCTATGTTTCGCTACGACAGACCACAAGCGGGCAGATATCGTCAGTTCCACCAATTTGGTGTTGAAGTATTTGGAA
GTTTAGAACCAGAAACAGATGTGGAAGTAATGAAACTGTTGTTAGACTATTTTGAGGCACTGGGATTATCGGGATTAGAG
CTCAAACTTAATAGTGTTGGTTGTAAAGAATGTCGCCAAAAATACTTAAATGAATTAAGCAATTATTTTGAAGGGAAGAT
AGATCATATTTGTCAGGATTGTTATCAAAGGTATCAAACTAATCCCATGAGAGTCTTGGATTGTAAAAAGAAATCTTGTC
GTGAAGCTATTGGTGAAGATGTGCCTTTAATAGTAAATTATTTATGTCAAGATTGTGCTACTCACTTTGATAAAGTTAAA
GAAATTTTAACTAGCTTGAATTTACCATACCAGATTGACCCTCATCTAGTTCGTGGTTTAGATTATTATACCAAGACAGC
TTTTGAAGTAGTAATGCCTTCTTTGGGGGCACAAGATGCCCTAGGGGGTGGAGGCAGATACGATTATTTAGTAGAAGAAT
GTGGAGGCCCGCCCACCCCTGGTGTAGGTTTTGCAGTAGGGCTAGAGCGAATTTTATTAGCCCTCGAGCAAAAAGAGATC
AACTTAGATCAAGAAGAAAAATTAGATTTCTATTTTATTGCCACTGGGGAAGAAGCAAAACAAGAAGCATTTAAATTGTT
GGCTACAGTTAGAGAAGAGGGTTATAGCGCAGTTATGGATTTTGAACGACGTAGTATGAGAGCGCAAATGAAAAGAGCAA
ATAAGGCAAATGCCAGATTTTCTTTGATCCTTGGTGAAGCAGAATTGAACGAACAAACATGTCAAATTAAGGATATGGAA
GCAGGCGAACAATTTCAAGTTCCTTTAAAGAGCTTTGAAGAAAGGATAAAAGAGATAGCTGGTTAA

Upstream 100 bases:

>100_bases
GACGGGAGTTTTTTAGTTTTGGGCTGATTAATAAAGATCGTATTCCAGGAATTTATATTAAGATATTAATGCTAGTTTTA
TTTTGAAAGGGGGAATTGTT

Downstream 100 bases:

>100_bases
AACAAATTAAGGATAGGGGGTTTGTAATATGAAAAGAACACATTACTGTGGAGAATTGACCAGGGAATTAGTTGGGAAAA
AAGTTGTCCTTAAGGGATGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MLTKAPRGTKDILPQESSKWHALEETAKKISNLYGFNEVRFPIFEHTELFTRSVGETSDIVSKEMYTFTDRGDRSLTLRP
EGTAPAARLYVEHKLFNEPQPVKYYYIGPMFRYDRPQAGRYRQFHQFGVEVFGSLEPETDVEVMKLLLDYFEALGLSGLE
LKLNSVGCKECRQKYLNELSNYFEGKIDHICQDCYQRYQTNPMRVLDCKKKSCREAIGEDVPLIVNYLCQDCATHFDKVK
EILTSLNLPYQIDPHLVRGLDYYTKTAFEVVMPSLGAQDALGGGGRYDYLVEECGGPPTPGVGFAVGLERILLALEQKEI
NLDQEEKLDFYFIATGEEAKQEAFKLLATVREEGYSAVMDFERRSMRAQMKRANKANARFSLILGEAELNEQTCQIKDME
AGEQFQVPLKSFEERIKEIAG

Sequences:

>Translated_421_residues
MLTKAPRGTKDILPQESSKWHALEETAKKISNLYGFNEVRFPIFEHTELFTRSVGETSDIVSKEMYTFTDRGDRSLTLRP
EGTAPAARLYVEHKLFNEPQPVKYYYIGPMFRYDRPQAGRYRQFHQFGVEVFGSLEPETDVEVMKLLLDYFEALGLSGLE
LKLNSVGCKECRQKYLNELSNYFEGKIDHICQDCYQRYQTNPMRVLDCKKKSCREAIGEDVPLIVNYLCQDCATHFDKVK
EILTSLNLPYQIDPHLVRGLDYYTKTAFEVVMPSLGAQDALGGGGRYDYLVEECGGPPTPGVGFAVGLERILLALEQKEI
NLDQEEKLDFYFIATGEEAKQEAFKLLATVREEGYSAVMDFERRSMRAQMKRANKANARFSLILGEAELNEQTCQIKDME
AGEQFQVPLKSFEERIKEIAG
>Mature_421_residues
MLTKAPRGTKDILPQESSKWHALEETAKKISNLYGFNEVRFPIFEHTELFTRSVGETSDIVSKEMYTFTDRGDRSLTLRP
EGTAPAARLYVEHKLFNEPQPVKYYYIGPMFRYDRPQAGRYRQFHQFGVEVFGSLEPETDVEVMKLLLDYFEALGLSGLE
LKLNSVGCKECRQKYLNELSNYFEGKIDHICQDCYQRYQTNPMRVLDCKKKSCREAIGEDVPLIVNYLCQDCATHFDKVK
EILTSLNLPYQIDPHLVRGLDYYTKTAFEVVMPSLGAQDALGGGGRYDYLVEECGGPPTPGVGFAVGLERILLALEQKEI
NLDQEEKLDFYFIATGEEAKQEAFKLLATVREEGYSAVMDFERRSMRAQMKRANKANARFSLILGEAELNEQTCQIKDME
AGEQFQVPLKSFEERIKEIAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=459, Percent_Identity=24.8366013071895, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI15029520, Length=458, Percent_Identity=25.9825327510917, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=41.9431279620853, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71993693, Length=456, Percent_Identity=22.3684210526316, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=456, Percent_Identity=22.3684210526316, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=468, Percent_Identity=26.2820512820513, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24643061, Length=471, Percent_Identity=23.1422505307856, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24643059, Length=490, Percent_Identity=22.2448979591837, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_NATTJ (B2A2F9)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917462.1
- ProteinModelPortal:   B2A2F9
- SMR:   B2A2F9
- GeneID:   6315184
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1291
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48187; Mature: 48187

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKAPRGTKDILPQESSKWHALEETAKKISNLYGFNEVRFPIFEHTELFTRSVGETSDI
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHH
VSKEMYTFTDRGDRSLTLRPEGTAPAARLYVEHKLFNEPQPVKYYYIGPMFRYDRPQAGR
HHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCH
YRQFHQFGVEVFGSLEPETDVEVMKLLLDYFEALGLSGLELKLNSVGCKECRQKYLNELS
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
NYFEGKIDHICQDCYQRYQTNPMRVLDCKKKSCREAIGEDVPLIVNYLCQDCATHFDKVK
HHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EILTSLNLPYQIDPHLVRGLDYYTKTAFEVVMPSLGAQDALGGGGRYDYLVEECGGPPTP
HHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCC
GVGFAVGLERILLALEQKEINLDQEEKLDFYFIATGEEAKQEAFKLLATVREEGYSAVMD
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
FERRSMRAQMKRANKANARFSLILGEAELNEQTCQIKDMEAGEQFQVPLKSFEERIKEIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MLTKAPRGTKDILPQESSKWHALEETAKKISNLYGFNEVRFPIFEHTELFTRSVGETSDI
CCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHH
VSKEMYTFTDRGDRSLTLRPEGTAPAARLYVEHKLFNEPQPVKYYYIGPMFRYDRPQAGR
HHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCH
YRQFHQFGVEVFGSLEPETDVEVMKLLLDYFEALGLSGLELKLNSVGCKECRQKYLNELS
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
NYFEGKIDHICQDCYQRYQTNPMRVLDCKKKSCREAIGEDVPLIVNYLCQDCATHFDKVK
HHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
EILTSLNLPYQIDPHLVRGLDYYTKTAFEVVMPSLGAQDALGGGGRYDYLVEECGGPPTP
HHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCC
GVGFAVGLERILLALEQKEINLDQEEKLDFYFIATGEEAKQEAFKLLATVREEGYSAVMD
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
FERRSMRAQMKRANKANARFSLILGEAELNEQTCQIKDMEAGEQFQVPLKSFEERIKEIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA