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The map label for this gene is ptxB [H]

Identifier: 188585288

GI number: 188585288

Start: 673878

End: 674873

Strand: Reverse

Name: ptxB [H]

Synonym: Nther_0650

Alternate gene names: 188585288

Gene position: 674873-673878 (Counterclockwise)

Preceding gene: 188585293

Following gene: 188585287

Centisome position: 21.32

GC content: 34.74

Gene sequence:

>996_bases
TTGCCTGGCTTTCAAAAAAAGGTGTTTTTCGGAGTAGGTCTCTTATTATCCATCACAGTAATTGTTGGAATTTTCTTACC
TTTTTACGAAAATTATCAATTACAGCAGTATCCTAAAGTGAATCTAACATCAAAGGATCAGAACCCTTCCGAATTTCAAG
AAATAACTTCCCAAGATACAAAAGAATTTAACAAAGAGAGAGACAATGACTCTCATTTATTAAGAGTTGGCCTGGGAGCA
ATAACAGCAGGTCGTGAATCCCTTTATTACTATAAAGAACTATTAGACCATTTAGAAGATCAGCTAGGGATGGAAGTTAA
AATCCATCAAACTCGTACCCATTCAGAAATGAATGAATTAATACGCCATGGTAACATCGATATAGCATTCATTTGTTCTT
ATTCTTACATTCAATTACAAGATAGCCATGGAGTGCCATTATTGGTCGTACCTGAGATAAATGGAACCACCACATATAGA
TCTGTAATTCTAGCAAGAAAAGAGCTTAATATAGATGCTTTTAATCAATTGGAGAATAAAACTTTTGCTTTTACAGATCC
CCTATCTAATGCAGGCAAACTATATCCTGCATATTTACTAAAACAACAAGGACAAAGTCCCCAGGATTTTTTTGAACACA
GTTATTTCACATATAGCCACAATAATTCTGTAGATAGTGTGATAGAAGGGGTTGTGGATGCCGCTGCTGTTGATAGTCTT
GTATATGACTACTTGTATACTAAAGATCCAGCTAGATTTGAAAATGTGGAAATAATTCATACTTCACCTGCTTTTGAAAT
CTCACCTGTTGTGGCTAGTCCCAAATTAAAAACTTCATTAAAAGAAGATATTAAAAACTTTTTCTTGGAATTACATGAAA
CTTCTAATGGTCAATATATATTGGAGCAATTGGGAATTGAGCAGTTTGAGCCCGCTCAAGATAGTAGCTATGACTCCATC
AGAGATATGGCCGAGGTGGTAGGTTATGATAAATAA

Upstream 100 bases:

>100_bases
CAAATTTAAATATGTAAGGGTTTATAGTAAAATATATTTAACACTTTAAGTGAGGATAAATTATCATTTATCTACTGATA
TTAGAGTAGGGGGTATCGGA

Downstream 100 bases:

>100_bases
AATTAAAAATAAAATTGGTATTAAAGAGAAAATAATAGCTATTGTAGTTATAATTGTATTAATGCTTGGGATTTGGACTG
TAGTTCAAGTCAGGTCAACT

Product: phosphonate ABC transporter, periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MPGFQKKVFFGVGLLLSITVIVGIFLPFYENYQLQQYPKVNLTSKDQNPSEFQEITSQDTKEFNKERDNDSHLLRVGLGA
ITAGRESLYYYKELLDHLEDQLGMEVKIHQTRTHSEMNELIRHGNIDIAFICSYSYIQLQDSHGVPLLVVPEINGTTTYR
SVILARKELNIDAFNQLENKTFAFTDPLSNAGKLYPAYLLKQQGQSPQDFFEHSYFTYSHNNSVDSVIEGVVDAAAVDSL
VYDYLYTKDPARFENVEIIHTSPAFEISPVVASPKLKTSLKEDIKNFFLELHETSNGQYILEQLGIEQFEPAQDSSYDSI
RDMAEVVGYDK

Sequences:

>Translated_331_residues
MPGFQKKVFFGVGLLLSITVIVGIFLPFYENYQLQQYPKVNLTSKDQNPSEFQEITSQDTKEFNKERDNDSHLLRVGLGA
ITAGRESLYYYKELLDHLEDQLGMEVKIHQTRTHSEMNELIRHGNIDIAFICSYSYIQLQDSHGVPLLVVPEINGTTTYR
SVILARKELNIDAFNQLENKTFAFTDPLSNAGKLYPAYLLKQQGQSPQDFFEHSYFTYSHNNSVDSVIEGVVDAAAVDSL
VYDYLYTKDPARFENVEIIHTSPAFEISPVVASPKLKTSLKEDIKNFFLELHETSNGQYILEQLGIEQFEPAQDSSYDSI
RDMAEVVGYDK
>Mature_330_residues
PGFQKKVFFGVGLLLSITVIVGIFLPFYENYQLQQYPKVNLTSKDQNPSEFQEITSQDTKEFNKERDNDSHLLRVGLGAI
TAGRESLYYYKELLDHLEDQLGMEVKIHQTRTHSEMNELIRHGNIDIAFICSYSYIQLQDSHGVPLLVVPEINGTTTYRS
VILARKELNIDAFNQLENKTFAFTDPLSNAGKLYPAYLLKQQGQSPQDFFEHSYFTYSHNNSVDSVIEGVVDAAAVDSLV
YDYLYTKDPARFENVEIIHTSPAFEISPVVASPKLKTSLKEDIKNFFLELHETSNGQYILEQLGIEQFEPAQDSSYDSIR
DMAEVVGYDK

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=240, Percent_Identity=25.8333333333333, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 37758; Mature: 37627

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00088 SOD_MN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGFQKKVFFGVGLLLSITVIVGIFLPFYENYQLQQYPKVNLTSKDQNPSEFQEITSQDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KEFNKERDNDSHLLRVGLGAITAGRESLYYYKELLDHLEDQLGMEVKIHQTRTHSEMNEL
HHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
IRHGNIDIAFICSYSYIQLQDSHGVPLLVVPEINGTTTYRSVILARKELNIDAFNQLENK
HHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCC
TFAFTDPLSNAGKLYPAYLLKQQGQSPQDFFEHSYFTYSHNNSVDSVIEGVVDAAAVDSL
EEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
VYDYLYTKDPARFENVEIIHTSPAFEISPVVASPKLKTSLKEDIKNFFLELHETSNGQYI
HHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
LEQLGIEQFEPAQDSSYDSIRDMAEVVGYDK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PGFQKKVFFGVGLLLSITVIVGIFLPFYENYQLQQYPKVNLTSKDQNPSEFQEITSQDT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KEFNKERDNDSHLLRVGLGAITAGRESLYYYKELLDHLEDQLGMEVKIHQTRTHSEMNEL
HHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
IRHGNIDIAFICSYSYIQLQDSHGVPLLVVPEINGTTTYRSVILARKELNIDAFNQLENK
HHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCC
TFAFTDPLSNAGKLYPAYLLKQQGQSPQDFFEHSYFTYSHNNSVDSVIEGVVDAAAVDSL
EEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
VYDYLYTKDPARFENVEIIHTSPAFEISPVVASPKLKTSLKEDIKNFFLELHETSNGQYI
HHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
LEQLGIEQFEPAQDSSYDSIRDMAEVVGYDK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]