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The map label for this gene is rnfA [H]

Identifier: 188585137

GI number: 188585137

Start: 526291

End: 526866

Strand: Direct

Name: rnfA [H]

Synonym: Nther_0498

Alternate gene names: 188585137

Gene position: 526291-526866 (Clockwise)

Preceding gene: 188585136

Following gene: 188585138

Centisome position: 16.63

GC content: 35.07

Gene sequence:

>576_bases
ATGGAAGAATTATTGGCGATATTTATTGGCGCCATTTTTGTCAATAATATAGTATTGATTCAGTTTTTGGGAATATGTCC
TTTTTTAGGAGTTTCCAAAAAAGTAGAAACAGCAGTGGGAATGGGCTTAGCCGTAACTTTTATTATGACCTTGGCTTCAA
TTATTACCTGGTTAATCCATGAAATGATTTTGGTGCCCTTTGAAGCTGAGTATCTAGAATATGTTGCATATATTCTAGTA
ATTGCTTCCCTAGTACAGTTTATTGAGATGTTACTTGAAAAGGTAAGTCCTAATTTATATAAAGCACTTGGGATCTATTT
GCCTTTAATAACTACCAACTGTGCTATTTTAGGCCTAGCTTTGCAAAACATTTTAGAAGGTTACAACTTACTAGAATCTA
CAATTCATGGCGTTGGAGCTGGCATAGGCTTTACTGTAGCCCTGGTAGTTATGGCAGGTATTAGAGAACAGTTGGAATTC
GCTAAAATGCCCAATTTATTTCAAGGTGCTTCAGTGGCATTTATTATAGCAGGAATTCTTTCCATTGCTTTTACTGGTTT
TCTTGGTGTAATATAA

Upstream 100 bases:

>100_bases
GCTGCTATTACTTAAAGTACCTTGTAAGTCGATATAGTAATACAGGGAGAGTAAGTGATTACTGAGTTGGTAGTAACTAG
AGTGGAAAGGGGGCTTAATA

Downstream 100 bases:

>100_bases
TGAATAGAGGTAGAGGTTGAACTTGGGAGGTGAAATAATTGTATGCAGCAGTACTGAGTCTAGGAGGATTAGGGCTGTTT
TTTGGCCTGGTGCTAGCTTA

Product: electron transport complex, RnfABCDGE type, A subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfA [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEELLAIFIGAIFVNNIVLIQFLGICPFLGVSKKVETAVGMGLAVTFIMTLASIITWLIHEMILVPFEAEYLEYVAYILV
IASLVQFIEMLLEKVSPNLYKALGIYLPLITTNCAILGLALQNILEGYNLLESTIHGVGAGIGFTVALVVMAGIREQLEF
AKMPNLFQGASVAFIIAGILSIAFTGFLGVI

Sequences:

>Translated_191_residues
MEELLAIFIGAIFVNNIVLIQFLGICPFLGVSKKVETAVGMGLAVTFIMTLASIITWLIHEMILVPFEAEYLEYVAYILV
IASLVQFIEMLLEKVSPNLYKALGIYLPLITTNCAILGLALQNILEGYNLLESTIHGVGAGIGFTVALVVMAGIREQLEF
AKMPNLFQGASVAFIIAGILSIAFTGFLGVI
>Mature_191_residues
MEELLAIFIGAIFVNNIVLIQFLGICPFLGVSKKVETAVGMGLAVTFIMTLASIITWLIHEMILVPFEAEYLEYVAYILV
IASLVQFIEMLLEKVSPNLYKALGIYLPLITTNCAILGLALQNILEGYNLLESTIHGVGAGIGFTVALVVMAGIREQLEF
AKMPNLFQGASVAFIIAGILSIAFTGFLGVI

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=191, Percent_Identity=55.4973821989529, Blast_Score=222, Evalue=1e-59,
Organism=Escherichia coli, GI1787919, Length=176, Percent_Identity=29.5454545454545, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20645; Mature: 20645

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELLAIFIGAIFVNNIVLIQFLGICPFLGVSKKVETAVGMGLAVTFIMTLASIITWLIH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMILVPFEAEYLEYVAYILVIASLVQFIEMLLEKVSPNLYKALGIYLPLITTNCAILGLA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQNILEGYNLLESTIHGVGAGIGFTVALVVMAGIREQLEFAKMPNLFQGASVAFIIAGIL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHH
SIAFTGFLGVI
HHHHHHHHHCC
>Mature Secondary Structure
MEELLAIFIGAIFVNNIVLIQFLGICPFLGVSKKVETAVGMGLAVTFIMTLASIITWLIH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMILVPFEAEYLEYVAYILVIASLVQFIEMLLEKVSPNLYKALGIYLPLITTNCAILGLA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQNILEGYNLLESTIHGVGAGIGFTVALVVMAGIREQLEFAKMPNLFQGASVAFIIAGIL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHH
SIAFTGFLGVI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA