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The map label for this gene is purN [H]

Identifier: 188585096

GI number: 188585096

Start: 482195

End: 482818

Strand: Direct

Name: purN [H]

Synonym: Nther_0457

Alternate gene names: 188585096

Gene position: 482195-482818 (Clockwise)

Preceding gene: 188585095

Following gene: 188585097

Centisome position: 15.23

GC content: 37.18

Gene sequence:

>624_bases
ATGGAGATAAATAAAGATCCTAGATATGCTGTATTAGCCTCAGGATCGGGAACAATTTTTCAATCTATAATTGATGCTCA
AAAGCGAGGAGATATTCCCGGGGAACTGGCTCTTTTTTTGACCGATAAACAAGATTGTCAAGCAAAAACGAGGGCAGAAA
AAGCTGGGATTGAGACCAGAGTGTTTCAACCTAAAAATTATACAAGTAAACAGGCTATGGAAGAGGAAATGTTGGCAGTG
TTAACTGCTCAAGAAATAGATTATGTAGTTTTAGCTGGTTATTTAAGAATCTTATCTCCTGAATTCATTAGGAACTTTCG
CCATCGGATTATCAATACTCATCCTTCGTTATTACCTGCATTTAAAGGGCTTGATGCCGTAAAGCAAGCCTACGATCACG
GTGTTAAAGTAACTGGCTGTACTGTGCATTTAGTTACAGAAGAATTGGATTCAGGTCCTATATTACTTCAGGAAGAAGTA
AAAGTTCAACGACATGATAGTTTGGATGAGCTCAGGGAAAAGATAAAAAATAAAGAAAGACGGTTAATTATAACTGCCAT
AAGAGCGCTTTTAAAAGGTGAAGTTATTGTAGACAATCACAAAAGGTGGGTGGTGGAAAATTGA

Upstream 100 bases:

>100_bases
AGAGAGCAGGGAGAAAAGGCTGAAATTATTGGGACGGTAGAACAACTATCCCAACAACAGGGTGATGGTGTTCAGAGTGG
AGAAAGGGTGATGTTTGTAA

Downstream 100 bases:

>100_bases
CTATCCAAAAAATAAACAGGGTTTTATTAAGTGTATATGATAAAACCGGCCTAATAACTCTTGCACAGGAATTGAAAGAC
ATGGGGGTAGAACTTGTTTC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MEINKDPRYAVLASGSGTIFQSIIDAQKRGDIPGELALFLTDKQDCQAKTRAEKAGIETRVFQPKNYTSKQAMEEEMLAV
LTAQEIDYVVLAGYLRILSPEFIRNFRHRIINTHPSLLPAFKGLDAVKQAYDHGVKVTGCTVHLVTEELDSGPILLQEEV
KVQRHDSLDELREKIKNKERRLIITAIRALLKGEVIVDNHKRWVVEN

Sequences:

>Translated_207_residues
MEINKDPRYAVLASGSGTIFQSIIDAQKRGDIPGELALFLTDKQDCQAKTRAEKAGIETRVFQPKNYTSKQAMEEEMLAV
LTAQEIDYVVLAGYLRILSPEFIRNFRHRIINTHPSLLPAFKGLDAVKQAYDHGVKVTGCTVHLVTEELDSGPILLQEEV
KVQRHDSLDELREKIKNKERRLIITAIRALLKGEVIVDNHKRWVVEN
>Mature_207_residues
MEINKDPRYAVLASGSGTIFQSIIDAQKRGDIPGELALFLTDKQDCQAKTRAEKAGIETRVFQPKNYTSKQAMEEEMLAV
LTAQEIDYVVLAGYLRILSPEFIRNFRHRIINTHPSLLPAFKGLDAVKQAYDHGVKVTGCTVHLVTEELDSGPILLQEEV
KVQRHDSLDELREKIKNKERRLIITAIRALLKGEVIVDNHKRWVVEN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=38.7254901960784, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=38.7254901960784, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=38.7254901960784, Blast_Score=151, Evalue=3e-37,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=36.0824742268041, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1787483, Length=129, Percent_Identity=36.4341085271318, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=38.7978142076503, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=209, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=36.2694300518135, Blast_Score=134, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23488; Mature: 23488

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINKDPRYAVLASGSGTIFQSIIDAQKRGDIPGELALFLTDKQDCQAKTRAEKAGIETR
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEE
VFQPKNYTSKQAMEEEMLAVLTAQEIDYVVLAGYLRILSPEFIRNFRHRIINTHPSLLPA
EECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
FKGLDAVKQAYDHGVKVTGCTVHLVTEELDSGPILLQEEVKVQRHDSLDELREKIKNKER
HCCHHHHHHHHHCCCEEECEEEEEEEHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHCHHH
RLIITAIRALLKGEVIVDNHKRWVVEN
HHHHHHHHHHHCCCEEEECCCEEEECC
>Mature Secondary Structure
MEINKDPRYAVLASGSGTIFQSIIDAQKRGDIPGELALFLTDKQDCQAKTRAEKAGIETR
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEE
VFQPKNYTSKQAMEEEMLAVLTAQEIDYVVLAGYLRILSPEFIRNFRHRIINTHPSLLPA
EECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
FKGLDAVKQAYDHGVKVTGCTVHLVTEELDSGPILLQEEVKVQRHDSLDELREKIKNKER
HCCHHHHHHHHHCCCEEECEEEEEEEHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHCHHH
RLIITAIRALLKGEVIVDNHKRWVVEN
HHHHHHHHHHHCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]