Click here to switch to the map view.

The map label for this gene is purH

Identifier: 188585097

GI number: 188585097

Start: 482815

End: 484344

Strand: Direct

Name: purH

Synonym: Nther_0458

Alternate gene names: 188585097

Gene position: 482815-484344 (Clockwise)

Preceding gene: 188585096

Following gene: 188585098

Centisome position: 15.25

GC content: 37.65

Gene sequence:

>1530_bases
TTGACTATCCAAAAAATAAACAGGGTTTTATTAAGTGTATATGATAAAACCGGCCTAATAACTCTTGCACAGGAATTGAA
AGACATGGGGGTAGAACTTGTTTCTACGGGAGGTACTTTGCGTCATTTACAAACCCATGGAATTCCAGTCTATTCAGTGG
AAGAAGTAACCGGTTTTCCAGAAATATTATCAGGTCGAGTAAAAACTCTTCACCCTAAGATTCACGGCGGTATCCTGGCT
AAAAATAGAGAAAATGAAGAATTATCGTCATTGGATATTAAAACTTTTGATCTAGTAATTGTCAATTTATACCCTTTTGA
GCAAGTAATGCAAAAAGCTGAAGCTACTGAAGAAGAAGTGATGGAAAATATTGATATTGGTGGACCTACTATGATAAGAG
CTGCTGCTAAAAATTGGTATCGAGTTGGTGTATGCGTTGATCCTACTGATTATGAAGTGCTAACACAGCAACTAAAACAA
TATCATGGATTAACAGATGAATTCAGAAAAACTTTGGCCAGGAAAGCATTTAAACATACAGCTCAATATGACAAAGCTAT
TTTCAATTATTTTGAAAGTGTTGATACTAACAAAGATGATGAAACGGAATTGAAATTTCCTGAATTTAATTTGTTAGAAT
CCCGGGAGCTACCTTATGGTGAAAATCCACATCAAAAGGCCAGCCTATTAATGGAGAGTCAAAAATTTATCCAACATCAA
GGTAAGGGTCTCTCTTACAACAATTTCCAGGACATAGATGCTGCCATAAAGTTAGTACATGAATTCCAAAAACCAGCAGT
GGTAGCCGTCAAGCATACTAATCCTTGCGGTGTCGGAGTTTCAAATACTATTGAGGAAGCCTATGATAAAGCATACCAAG
GTGATCCTGTGAGCATCTTTGGCGGAATAGTGGCATGTAATCGTCCTGTAACGGAAGAACTTGCTTCCAAGCTGACAGAA
ATTTTCTTGGATGTGATAATTGCACCAGAATTTGAGCCTCGCGCCCTAGAAAAATTGAAGTCAAAATCAGGTACTAAAGT
TGTGGAGATGGATTTGGAAAAGATGGTTGGAAATAAAGTGGAGATAAAATCTACAACCTTTGGATATTTATGTCAAGAAG
CTGATTATCATCATCCTCATCCCCAAAATTGGGATAGAGTTGCAGGGGAACAGGCTAAACCAGAAGAGATAGATGATTTG
ATAATTGCCGAAAAAATAGTCAAACATGTAAAATCTAATGCCATTGTAGTTGTAAAAGAGGGTCAATCTCTGGGAATAGG
AGCAGGTCAAATGAACAGGGTAGGGGCTTCTAGAATCGCCTTAGAAAATGCCGGTAAGGAATCCCAAAACAGTGTTTTGG
CATCAGATGCCTTTTTCCCATTTAATGATGTTGTGAAATTATGCAGTCAGTACGGTGTCTCCGCTATTGTACAGCCGGGA
GGTTCGAAAAGAGATCAAGATTCAATTGATCTTGCTCAAGAAACAGGAATCACCATGTATTTTACAGGTATACGTCATTT
TAAACATTAA

Upstream 100 bases:

>100_bases
AGATAAAAAATAAAGAAAGACGGTTAATTATAACTGCCATAAGAGCGCTTTTAAAAGGTGAAGTTATTGTAGACAATCAC
AAAAGGTGGGTGGTGGAAAA

Downstream 100 bases:

>100_bases
TTTTAAACTGAAAGCTGGGGGGTTTAAGGTGAAAGTTCTGGTAATTGGTTCAGGAGGGCGTGAACATGCCCTGGTTAAAA
AAATAGCAGAAAGTAGTCGG

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTIQKINRVLLSVYDKTGLITLAQELKDMGVELVSTGGTLRHLQTHGIPVYSVEEVTGFPEILSGRVKTLHPKIHGGILA
KNRENEELSSLDIKTFDLVIVNLYPFEQVMQKAEATEEEVMENIDIGGPTMIRAAAKNWYRVGVCVDPTDYEVLTQQLKQ
YHGLTDEFRKTLARKAFKHTAQYDKAIFNYFESVDTNKDDETELKFPEFNLLESRELPYGENPHQKASLLMESQKFIQHQ
GKGLSYNNFQDIDAAIKLVHEFQKPAVVAVKHTNPCGVGVSNTIEEAYDKAYQGDPVSIFGGIVACNRPVTEELASKLTE
IFLDVIIAPEFEPRALEKLKSKSGTKVVEMDLEKMVGNKVEIKSTTFGYLCQEADYHHPHPQNWDRVAGEQAKPEEIDDL
IIAEKIVKHVKSNAIVVVKEGQSLGIGAGQMNRVGASRIALENAGKESQNSVLASDAFFPFNDVVKLCSQYGVSAIVQPG
GSKRDQDSIDLAQETGITMYFTGIRHFKH

Sequences:

>Translated_509_residues
MTIQKINRVLLSVYDKTGLITLAQELKDMGVELVSTGGTLRHLQTHGIPVYSVEEVTGFPEILSGRVKTLHPKIHGGILA
KNRENEELSSLDIKTFDLVIVNLYPFEQVMQKAEATEEEVMENIDIGGPTMIRAAAKNWYRVGVCVDPTDYEVLTQQLKQ
YHGLTDEFRKTLARKAFKHTAQYDKAIFNYFESVDTNKDDETELKFPEFNLLESRELPYGENPHQKASLLMESQKFIQHQ
GKGLSYNNFQDIDAAIKLVHEFQKPAVVAVKHTNPCGVGVSNTIEEAYDKAYQGDPVSIFGGIVACNRPVTEELASKLTE
IFLDVIIAPEFEPRALEKLKSKSGTKVVEMDLEKMVGNKVEIKSTTFGYLCQEADYHHPHPQNWDRVAGEQAKPEEIDDL
IIAEKIVKHVKSNAIVVVKEGQSLGIGAGQMNRVGASRIALENAGKESQNSVLASDAFFPFNDVVKLCSQYGVSAIVQPG
GSKRDQDSIDLAQETGITMYFTGIRHFKH
>Mature_508_residues
TIQKINRVLLSVYDKTGLITLAQELKDMGVELVSTGGTLRHLQTHGIPVYSVEEVTGFPEILSGRVKTLHPKIHGGILAK
NRENEELSSLDIKTFDLVIVNLYPFEQVMQKAEATEEEVMENIDIGGPTMIRAAAKNWYRVGVCVDPTDYEVLTQQLKQY
HGLTDEFRKTLARKAFKHTAQYDKAIFNYFESVDTNKDDETELKFPEFNLLESRELPYGENPHQKASLLMESQKFIQHQG
KGLSYNNFQDIDAAIKLVHEFQKPAVVAVKHTNPCGVGVSNTIEEAYDKAYQGDPVSIFGGIVACNRPVTEELASKLTEI
FLDVIIAPEFEPRALEKLKSKSGTKVVEMDLEKMVGNKVEIKSTTFGYLCQEADYHHPHPQNWDRVAGEQAKPEEIDDLI
IAEKIVKHVKSNAIVVVKEGQSLGIGAGQMNRVGASRIALENAGKESQNSVLASDAFFPFNDVVKLCSQYGVSAIVQPGG
SKRDQDSIDLAQETGITMYFTGIRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=474, Percent_Identity=35.6540084388186, Blast_Score=239, Evalue=4e-63,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=43.2380952380952, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71985564, Length=602, Percent_Identity=32.890365448505, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=28.1967213114754, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=468, Percent_Identity=33.974358974359, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323768, Length=471, Percent_Identity=32.484076433121, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24649832, Length=478, Percent_Identity=35.7740585774059, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_NATTJ (B2A5W1)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916642.1
- ProteinModelPortal:   B2A5W1
- SMR:   B2A5W1
- GeneID:   6315336
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0458
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56802; Mature: 56671

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQKINRVLLSVYDKTGLITLAQELKDMGVELVSTGGTLRHLQTHGIPVYSVEEVTGFP
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCEEHHHHCCCCEEEHHHHCCCH
EILSGRVKTLHPKIHGGILAKNRENEELSSLDIKTFDLVIVNLYPFEQVMQKAEATEEEV
HHHCCCHHEECCHHCCCEEECCCCCCHHHHCCCEEEEEEEEEECCHHHHHHHHHCCHHHH
MENIDIGGPTMIRAAAKNWYRVGVCVDPTDYEVLTQQLKQYHGLTDEFRKTLARKAFKHT
HHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQYDKAIFNYFESVDTNKDDETELKFPEFNLLESRELPYGENPHQKASLLMESQKFIQHQ
HHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GKGLSYNNFQDIDAAIKLVHEFQKPAVVAVKHTNPCGVGVSNTIEEAYDKAYQGDPVSIF
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
GGIVACNRPVTEELASKLTEIFLDVIIAPEFEPRALEKLKSKSGTKVVEMDLEKMVGNKV
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCEE
EIKSTTFGYLCQEADYHHPHPQNWDRVAGEQAKPEEIDDLIIAEKIVKHVKSNAIVVVKE
EEECCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GQSLGIGAGQMNRVGASRIALENAGKESQNSVLASDAFFPFNDVVKLCSQYGVSAIVQPG
CCCCCCCCCCHHHCCHHHHHHHCCCCCHHCCEEECCCCCCHHHHHHHHHHCCCEEEECCC
GSKRDQDSIDLAQETGITMYFTGIRHFKH
CCCCCCCHHHHHHHCCCEEEEECHHHHCC
>Mature Secondary Structure 
TIQKINRVLLSVYDKTGLITLAQELKDMGVELVSTGGTLRHLQTHGIPVYSVEEVTGFP
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCEEHHHHCCCCEEEHHHHCCCH
EILSGRVKTLHPKIHGGILAKNRENEELSSLDIKTFDLVIVNLYPFEQVMQKAEATEEEV
HHHCCCHHEECCHHCCCEEECCCCCCHHHHCCCEEEEEEEEEECCHHHHHHHHHCCHHHH
MENIDIGGPTMIRAAAKNWYRVGVCVDPTDYEVLTQQLKQYHGLTDEFRKTLARKAFKHT
HHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQYDKAIFNYFESVDTNKDDETELKFPEFNLLESRELPYGENPHQKASLLMESQKFIQHQ
HHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GKGLSYNNFQDIDAAIKLVHEFQKPAVVAVKHTNPCGVGVSNTIEEAYDKAYQGDPVSIF
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHH
GGIVACNRPVTEELASKLTEIFLDVIIAPEFEPRALEKLKSKSGTKVVEMDLEKMVGNKV
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCEE
EIKSTTFGYLCQEADYHHPHPQNWDRVAGEQAKPEEIDDLIIAEKIVKHVKSNAIVVVKE
EEECCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GQSLGIGAGQMNRVGASRIALENAGKESQNSVLASDAFFPFNDVVKLCSQYGVSAIVQPG
CCCCCCCCCCHHHCCHHHHHHHCCCCCHHCCEEECCCCCCHHHHHHHHHHCCCEEEECCC
GSKRDQDSIDLAQETGITMYFTGIRHFKH
CCCCCCCHHHHHHHCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA