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The map label for this gene is cobB2 [H]

Identifier: 188584793

GI number: 188584793

Start: 176512

End: 177270

Strand: Direct

Name: cobB2 [H]

Synonym: Nther_0151

Alternate gene names: 188584793

Gene position: 176512-177270 (Clockwise)

Preceding gene: 188584791

Following gene: 188584795

Centisome position: 5.58

GC content: 36.76

Gene sequence:

>759_bases
ATGGCTGATAACAAAAAATTTCAAGATCTAAAAGAAAAACTATTGTCTTCCGAGGAACCTTATGTACTAACTGGAGCCGG
AATCAGTACAGAAAGTGGTATACCAGATTTTAGAGGCAAAGATGGCTTGTGGACCAAAATTGATCCTATGCAATATTCTA
CTAGAGAAGTATTGATGTCGGTGCCGGAAAAATTTTATGAATATGGTTTCGAAAGATTTAAGCAACTGGCCAACAAAGAA
CCCAATCAGGGTCATAAGATTTTAGCTGATTTAGAAAAACATGGTGTGATAAGTGGTATAGTCACACAAAATATCGATGG
TCTTCATCAAAAAGCAGGTTCAAAACAAGTATTTGAAGTCCATGGTAATACCAGAAAGTGCTATTGTTTAGGATGTAATC
AAGAGTATCCTTTCCAAGAATTAAGCGATCAATTAGAAAAAGAACAAAAAGATGTCCCTAAATGTAAGGAATGTGGAGGT
ATGTTACGACCTGATATCATACTATTTGGTGATCAAATGCCAGATCTATTTTTTAAAGTTACCACAGTTTTGAAACAACG
GTGCGACTTTTTATTGGTTATAGGTACCAGTTTACAGGTGTACCCTGTGGCTGCCCTAGCTGAACTAGGTATTCCCATGG
GTATAATTAATCTTGAGGAAACCCCCTTTGATCGACAGGCAGAAGTAGTTATACAGGGTAAATGTGGAGAGACTCTGTCA
CAACTATGGGATCATATGAAAGACGAATTATCACAATAA

Upstream 100 bases:

>100_bases
CCGGGACCCCGTGAGATGGCCCTGTACCTAAGAATGTTACTTTCATATATTCATCCCTCATTTTCTTTTAATACTAATTA
CTGCGAAAGGAGGCCTATTA

Downstream 100 bases:

>100_bases
TGAAATCCAGTAGTTAATATAACAAATCAATATTATTCAGTTTGGGACATTTTTGAAGTGACAGAACTGACTTTTTGGTT
CATACTTGCTGTTTTATCTC

Product: Silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MADNKKFQDLKEKLLSSEEPYVLTGAGISTESGIPDFRGKDGLWTKIDPMQYSTREVLMSVPEKFYEYGFERFKQLANKE
PNQGHKILADLEKHGVISGIVTQNIDGLHQKAGSKQVFEVHGNTRKCYCLGCNQEYPFQELSDQLEKEQKDVPKCKECGG
MLRPDIILFGDQMPDLFFKVTTVLKQRCDFLLVIGTSLQVYPVAALAELGIPMGIINLEETPFDRQAEVVIQGKCGETLS
QLWDHMKDELSQ

Sequences:

>Translated_252_residues
MADNKKFQDLKEKLLSSEEPYVLTGAGISTESGIPDFRGKDGLWTKIDPMQYSTREVLMSVPEKFYEYGFERFKQLANKE
PNQGHKILADLEKHGVISGIVTQNIDGLHQKAGSKQVFEVHGNTRKCYCLGCNQEYPFQELSDQLEKEQKDVPKCKECGG
MLRPDIILFGDQMPDLFFKVTTVLKQRCDFLLVIGTSLQVYPVAALAELGIPMGIINLEETPFDRQAEVVIQGKCGETLS
QLWDHMKDELSQ
>Mature_251_residues
ADNKKFQDLKEKLLSSEEPYVLTGAGISTESGIPDFRGKDGLWTKIDPMQYSTREVLMSVPEKFYEYGFERFKQLANKEP
NQGHKILADLEKHGVISGIVTQNIDGLHQKAGSKQVFEVHGNTRKCYCLGCNQEYPFQELSDQLEKEQKDVPKCKECGGM
LRPDIILFGDQMPDLFFKVTTVLKQRCDFLLVIGTSLQVYPVAALAELGIPMGIINLEETPFDRQAEVVIQGKCGETLSQ
LWDHMKDELSQ

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=271, Percent_Identity=34.6863468634686, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI6912662, Length=272, Percent_Identity=34.9264705882353, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI300795542, Length=258, Percent_Identity=35.2713178294574, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI13787215, Length=252, Percent_Identity=34.9206349206349, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI13775602, Length=213, Percent_Identity=34.7417840375587, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI13775600, Length=213, Percent_Identity=34.7417840375587, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI6912660, Length=226, Percent_Identity=32.7433628318584, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI63054862, Length=207, Percent_Identity=33.8164251207729, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI7657575, Length=247, Percent_Identity=29.5546558704453, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI300797577, Length=259, Percent_Identity=30.5019305019305, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI300797705, Length=188, Percent_Identity=34.0425531914894, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7706712, Length=253, Percent_Identity=28.0632411067194, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI300797597, Length=259, Percent_Identity=28.957528957529, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI215982798, Length=199, Percent_Identity=27.6381909547739, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI308199517, Length=188, Percent_Identity=36.7021276595745, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541892, Length=233, Percent_Identity=33.4763948497854, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17567771, Length=273, Percent_Identity=30.03663003663, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71990482, Length=258, Percent_Identity=29.8449612403101, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71990487, Length=260, Percent_Identity=29.2307692307692, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325242, Length=209, Percent_Identity=35.4066985645933, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320163, Length=296, Percent_Identity=27.027027027027, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324599, Length=265, Percent_Identity=29.0566037735849, Blast_Score=92, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324504, Length=245, Percent_Identity=26.9387755102041, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320397, Length=224, Percent_Identity=26.3392857142857, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571445, Length=274, Percent_Identity=34.3065693430657, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137536, Length=257, Percent_Identity=29.1828793774319, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648389, Length=194, Percent_Identity=30.9278350515464, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571443, Length=214, Percent_Identity=31.7757009345794, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28571441, Length=214, Percent_Identity=31.7757009345794, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24650933, Length=236, Percent_Identity=29.2372881355932, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24645650, Length=252, Percent_Identity=27.3809523809524, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 28499; Mature: 28368

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNKKFQDLKEKLLSSEEPYVLTGAGISTESGIPDFRGKDGLWTKIDPMQYSTREVLMS
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHH
VPEKFYEYGFERFKQLANKEPNQGHKILADLEKHGVISGIVTQNIDGLHQKAGSKQVFEV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCCCEEEE
HGNTRKCYCLGCNQEYPFQELSDQLEKEQKDVPKCKECGGMLRPDIILFGDQMPDLFFKV
ECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHH
TTVLKQRCDFLLVIGTSLQVYPVAALAELGIPMGIINLEETPFDRQAEVVIQGKCGETLS
HHHHHHHCCEEEEECCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHH
QLWDHMKDELSQ
HHHHHHHHHHCC
>Mature Secondary Structure 
ADNKKFQDLKEKLLSSEEPYVLTGAGISTESGIPDFRGKDGLWTKIDPMQYSTREVLMS
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHH
VPEKFYEYGFERFKQLANKEPNQGHKILADLEKHGVISGIVTQNIDGLHQKAGSKQVFEV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCCCEEEE
HGNTRKCYCLGCNQEYPFQELSDQLEKEQKDVPKCKECGGMLRPDIILFGDQMPDLFFKV
ECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHH
TTVLKQRCDFLLVIGTSLQVYPVAALAELGIPMGIINLEETPFDRQAEVVIQGKCGETLS
HHHHHHHCCEEEEECCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHH
QLWDHMKDELSQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]