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The map label for this gene is dnaA

Identifier: 188528299

GI number: 188528299

Start: 1527250

End: 1528617

Strand: Reverse

Name: dnaA

Synonym: HPSH_07855

Alternate gene names: 188528299

Gene position: 1528617-1527250 (Counterclockwise)

Preceding gene: 188528305

Following gene: 188528296

Centisome position: 95.03

GC content: 40.06

Gene sequence:

>1368_bases
ATGGATACCAACAACAATATTGAAAAAGAAATCTTGGCGCTAATCAAACAAAAAGTTAGCCCCATAGAGTATGAAAATTG
CTTGAGCCAGCTCAAATACAACCCTAATGCAAGCAAGAGCGACATTGCCTTTTTTTATGCCCCTAACATGCTTTTATGCA
ATTGGATTACGGCCAAACACGGCGCGTTGCTTAAAGAAATTTTAAGCCAGAATAAAGTCGGCATGCATTTAGCCCATAGC
GTGGATGTGCGTATTGAAGTAGCGCCCAAAATCCAAATCAGCGCTCAACCTAACATCAATTACAAAGCGGTAAAAACGAG
CGTCAAAGACTCTTACACTTTTGAAAATTTTGTCGTAGGCTCATGCAATAACACCGTTTATGAAATCGCTAAAAAAGTCG
CCCAAAGCGATACCCCCCCTTATAACCCGGTGCTTTTTTATGGCGGCACAGGGTTAGGCAAAACGCACATTTTAAACGCT
ATCGGTAACCATGCCCTAGAAAAGCATAAAAAAGTCGTGTTAGTCACTTCAGAAGATTTTTTGACAGACTTCTTGAAGCA
TTTAGACAACCAAACCATGGATTCTTTTAAAAAAAAATACCGCCATTGCGACTTTTTCTTGTTAGATGACGCTCAATTTT
TGCAAGGAAAACCCAAGCTAGAAGAAGAATTTTTCCACACTTTTAACGAATTGCACGCCAACAGCAAACAAATCGTATTG
ATTTCAGACCGATCGCCTAAAAACATCGCCGGCTTAGAAGATCGCTTAAAATCGCGCTTTGAATGGGGGATAACCGCTAA
AGTCATGCCCCCTGATTTAGAAACCAAACTTTCCATTGTCAAACAAAAATGCCAGCTCAATAAAATCACTTTGCCTGAAG
AAGTGATGGAATACATCGCCCAACACATCAGCGACAATATCCGCCAAATGGAAGGCGCAATCATTAAAATCAGCGTGAAT
GCGAACTTAATGAACGCTCCCATTGATTTGAATCTCGCTAAAACCGTTTTAGAAGATTTGCAAAAAGATCATGCTGAAGG
CTCAAGCTTAGAAAATATCTTGCTCGCTGTCGCGCAAAGCCTGAATCTCAAATCCAGTGAAATCAAAATCTCTTCGCGCC
AAAAAAATGTCGCTCTAGCGAGAAAATTAGTCGTGTATTTCGCCAGGCTTTATACCCCTAACCCCACACTCTCGCTCGCT
CAATTTTTGGATTTAAAAGATCATTCTAGCATTTCTAAAATGTATTCTAGCGTTAAAAAAATGCTTGAAGAAGAAAAAAG
CCCCTTTGTCTTAAGCCTTAGAGAAGAAATCAAAAACCGCCTGAACGAATTGAACGACAAAAAAACCGCTTTCCATTCAA
GTGAATGA

Upstream 100 bases:

>100_bases
AAACCCTTAAAAGAAACACCTTAATTTTAAGGTTTCATTCACATTTCATTCACATGTTATTCCTTTTTCATTCACAACTT
ATTCACGCTATAATAACGCC

Downstream 100 bases:

>100_bases
AAAAAGGCTTATCAAAAAGCGTTTCATTCACTTTTTTTTACATCCCACAACCCCCTTAAAAACGCGCGTCTTTAAAGCTT
TTTCATTTTTCATTCCATTC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MDTNNNIEKEILALIKQKVSPIEYENCLSQLKYNPNASKSDIAFFYAPNMLLCNWITAKHGALLKEILSQNKVGMHLAHS
VDVRIEVAPKIQISAQPNINYKAVKTSVKDSYTFENFVVGSCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNA
IGNHALEKHKKVVLVTSEDFLTDFLKHLDNQTMDSFKKKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVL
ISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNKITLPEEVMEYIAQHISDNIRQMEGAIIKISVN
ANLMNAPIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQSLNLKSSEIKISSRQKNVALARKLVVYFARLYTPNPTLSLA
QFLDLKDHSSISKMYSSVKKMLEEEKSPFVLSLREEIKNRLNELNDKKTAFHSSE

Sequences:

>Translated_455_residues
MDTNNNIEKEILALIKQKVSPIEYENCLSQLKYNPNASKSDIAFFYAPNMLLCNWITAKHGALLKEILSQNKVGMHLAHS
VDVRIEVAPKIQISAQPNINYKAVKTSVKDSYTFENFVVGSCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNA
IGNHALEKHKKVVLVTSEDFLTDFLKHLDNQTMDSFKKKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVL
ISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNKITLPEEVMEYIAQHISDNIRQMEGAIIKISVN
ANLMNAPIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQSLNLKSSEIKISSRQKNVALARKLVVYFARLYTPNPTLSLA
QFLDLKDHSSISKMYSSVKKMLEEEKSPFVLSLREEIKNRLNELNDKKTAFHSSE
>Mature_455_residues
MDTNNNIEKEILALIKQKVSPIEYENCLSQLKYNPNASKSDIAFFYAPNMLLCNWITAKHGALLKEILSQNKVGMHLAHS
VDVRIEVAPKIQISAQPNINYKAVKTSVKDSYTFENFVVGSCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNA
IGNHALEKHKKVVLVTSEDFLTDFLKHLDNQTMDSFKKKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVL
ISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNKITLPEEVMEYIAQHISDNIRQMEGAIIKISVN
ANLMNAPIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQSLNLKSSEIKISSRQKNVALARKLVVYFARLYTPNPTLSLA
QFLDLKDHSSISKMYSSVKKMLEEEKSPFVLSLREEIKNRLNELNDKKTAFHSSE

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=322, Percent_Identity=37.5776397515528, Blast_Score=244, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51569; Mature: 51569

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTNNNIEKEILALIKQKVSPIEYENCLSQLKYNPNASKSDIAFFYAPNMLLCNWITAKH
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEHHHHHH
GALLKEILSQNKVGMHLAHSVDVRIEVAPKIQISAQPNINYKAVKTSVKDSYTFENFVVG
HHHHHHHHCCCCCCEEEEEEEEEEEEECCEEEEECCCCCCHHHHHHHHCCCCCCCCEEEE
SCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNAIGNHALEKHKKVVLVTSEDF
CCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECHHH
LTDFLKHLDNQTMDSFKKKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVL
HHHHHHHHCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
ISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNKITLPEEVMEYIA
EECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QHISDNIRQMEGAIIKISVNANLMNAPIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQS
HHHHHHHHHHCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LNLKSSEIKISSRQKNVALARKLVVYFARLYTPNPTLSLAQFLDLKDHSSISKMYSSVKK
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
MLEEEKSPFVLSLREEIKNRLNELNDKKTAFHSSE
HHHHCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MDTNNNIEKEILALIKQKVSPIEYENCLSQLKYNPNASKSDIAFFYAPNMLLCNWITAKH
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEHHHHHH
GALLKEILSQNKVGMHLAHSVDVRIEVAPKIQISAQPNINYKAVKTSVKDSYTFENFVVG
HHHHHHHHCCCCCCEEEEEEEEEEEEECCEEEEECCCCCCHHHHHHHHCCCCCCCCEEEE
SCNNTVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNAIGNHALEKHKKVVLVTSEDF
CCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECHHH
LTDFLKHLDNQTMDSFKKKYRHCDFFLLDDAQFLQGKPKLEEEFFHTFNELHANSKQIVL
HHHHHHHHCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
ISDRSPKNIAGLEDRLKSRFEWGITAKVMPPDLETKLSIVKQKCQLNKITLPEEVMEYIA
EECCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QHISDNIRQMEGAIIKISVNANLMNAPIDLNLAKTVLEDLQKDHAEGSSLENILLAVAQS
HHHHHHHHHHCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LNLKSSEIKISSRQKNVALARKLVVYFARLYTPNPTLSLAQFLDLKDHSSISKMYSSVKK
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
MLEEEKSPFVLSLREEIKNRLNELNDKKTAFHSSE
HHHHCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]