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The map label for this gene is 188528305

Identifier: 188528305

GI number: 188528305

Start: 1532623

End: 1533750

Strand: Reverse

Name: 188528305

Synonym: HPSH_07885

Alternate gene names: NA

Gene position: 1533750-1532623 (Counterclockwise)

Preceding gene: 188528306

Following gene: 188528299

Centisome position: 95.35

GC content: 32.18

Gene sequence:

>1128_bases
GTGATAGAACGCATGGACGCATTAATGTTGCCCTTAAATTGGCAAAGAGTGAGGCTTGGGGATATATTTTTCATTACGGC
TGGAGGCGATTTATCTAAGCCACATTATTCAAATACAAAACAAAGTGATTTTAATTACCCCATTTATTCAAATGCGATTG
AAAAAAAAGGTTTATGTGGATATTCATCTTTTTTTATTATTAAAAATAAGAGCATAACAATAACGGCTAGAGGAACAATA
GGTGTTGCTTTTTTTAGAGACTATCCTTATGTCCCTATCGGTAGACTTTTAGTCTTACAGCCAAAAATTAGTAATATTGA
TTGTAGGTTTTATGCGGAATATATCAATTCAAAAGTAAAATTTAACACCGAGCAAACTACTATTCCACAATTAACAATCC
CAAAAGTTGCACTATGTGAAATCCCCCTACCCCCTCTAAACGAACAGAACGCTATCGCTAACATTTTAAGCGCTTTGGAT
CGTTATCTTTGTGCATTAGACGCTCTCATTCTTAAAAAAGAGAGCGTTAAAAAAGCTCTAAGCTTTGAACTATTGAGCCA
AAAAAAACGCTTGAAAGGTTTCAATCAAGCTTGGCAAAGAGTGAGGCTTGGGGATATTGCCGAAATTAAAAGGGGTGTTC
GCATAACAAAAAATGAATTAGATGTATTTGGAAAATACCCTGTGGTTTCAGGTGGCGTTGGCTTTTTGGGTTACACAAAC
AACTTCAATAGATATGAAAATACAATTACTATCGCTCAATATGGAACGGCTGGCTATGTTAACTTTCAAAAAAATAAATT
TTGGGCTAATGATGTTTGTTTTTGTATATACCCTAATAAAGATATTATAAAAAATATTTTCTTGTATTATTTTTTGAAAG
TTAATCAAAATTATTTATATGAAATTTCAAATAGAAATGCTACACCTTATAGCATTTCAAAAGATAAAATTTTAGATTTT
GAAATCCTTTTACCCCCTCTAAACGAACAAATCGCTATCGCTAACATTTTAAGCGATTTGGATAATGAGATCGCCAGCCT
TAAAAACAAAAAACGCCAATTTGAAAACATCAAAAAAGCTTTAAACCACGATTTAATGAGCGCTAAAATTAGGGTTTTAA
AAAAATAA

Upstream 100 bases:

>100_bases
TCATTTCAAACTACTCTAACAGCTTGCTAGAATTAGACAAAGAAGTCAAAGAGAGCGAATCAAAAGTTTTAGAGCATTTG
AAAGATTTGGGGTTAATGGG

Downstream 100 bases:

>100_bases
ACGCCCCAAAAGTCAAGGACAAACCCGCTTCGCAAAGCAAACCCTAAGCTTTTAAGCTCAGGGGAACTTTGGCTAATAAT
TACTTTTCTATAGAAAAGAT

Product: type I R-M system specificity subunit

Products: NA

Alternate protein names: S.MpnORFAP; Type I restriction enzyme specificity protein MPN_089; S protein [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIERMDALMLPLNWQRVRLGDIFFITAGGDLSKPHYSNTKQSDFNYPIYSNAIEKKGLCGYSSFFIIKNKSITITARGTI
GVAFFRDYPYVPIGRLLVLQPKISNIDCRFYAEYINSKVKFNTEQTTIPQLTIPKVALCEIPLPPLNEQNAIANILSALD
RYLCALDALILKKESVKKALSFELLSQKKRLKGFNQAWQRVRLGDIAEIKRGVRITKNELDVFGKYPVVSGGVGFLGYTN
NFNRYENTITIAQYGTAGYVNFQKNKFWANDVCFCIYPNKDIIKNIFLYYFLKVNQNYLYEISNRNATPYSISKDKILDF
EILLPPLNEQIAIANILSDLDNEIASLKNKKRQFENIKKALNHDLMSAKIRVLKK

Sequences:

>Translated_375_residues
MIERMDALMLPLNWQRVRLGDIFFITAGGDLSKPHYSNTKQSDFNYPIYSNAIEKKGLCGYSSFFIIKNKSITITARGTI
GVAFFRDYPYVPIGRLLVLQPKISNIDCRFYAEYINSKVKFNTEQTTIPQLTIPKVALCEIPLPPLNEQNAIANILSALD
RYLCALDALILKKESVKKALSFELLSQKKRLKGFNQAWQRVRLGDIAEIKRGVRITKNELDVFGKYPVVSGGVGFLGYTN
NFNRYENTITIAQYGTAGYVNFQKNKFWANDVCFCIYPNKDIIKNIFLYYFLKVNQNYLYEISNRNATPYSISKDKILDF
EILLPPLNEQIAIANILSDLDNEIASLKNKKRQFENIKKALNHDLMSAKIRVLKK
>Mature_375_residues
MIERMDALMLPLNWQRVRLGDIFFITAGGDLSKPHYSNTKQSDFNYPIYSNAIEKKGLCGYSSFFIIKNKSITITARGTI
GVAFFRDYPYVPIGRLLVLQPKISNIDCRFYAEYINSKVKFNTEQTTIPQLTIPKVALCEIPLPPLNEQNAIANILSALD
RYLCALDALILKKESVKKALSFELLSQKKRLKGFNQAWQRVRLGDIAEIKRGVRITKNELDVFGKYPVVSGGVGFLGYTN
NFNRYENTITIAQYGTAGYVNFQKNKFWANDVCFCIYPNKDIIKNIFLYYFLKVNQNYLYEISNRNATPYSISKDKILDF
EILLPPLNEQIAIANILSDLDNEIASLKNKKRQFENIKKALNHDLMSAKIRVLKK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 43015; Mature: 43015

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERMDALMLPLNWQRVRLGDIFFITAGGDLSKPHYSNTKQSDFNYPIYSNAIEKKGLCG
CCCCCCEEECCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCHHHCCCCC
YSSFFIIKNKSITITARGTIGVAFFRDYPYVPIGRLLVLQPKISNIDCRFYAEYINSKVK
CCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHCCEEE
FNTEQTTIPQLTIPKVALCEIPLPPLNEQNAIANILSALDRYLCALDALILKKESVKKAL
ECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFELLSQKKRLKGFNQAWQRVRLGDIAEIKRGVRITKNELDVFGKYPVVSGGVGFLGYTN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCHHHHEECCCEEECCCEEEEECC
NFNRYENTITIAQYGTAGYVNFQKNKFWANDVCFCIYPNKDIIKNIFLYYFLKVNQNYLY
CCCCCCCEEEEEECCCEEEEEEECCCEECCCEEEEEECCHHHHHHHHHHEEEEECCCEEE
EISNRNATPYSISKDKILDFEILLPPLNEQIAIANILSDLDNEIASLKNKKRQFENIKKA
EEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNHDLMSAKIRVLKK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIERMDALMLPLNWQRVRLGDIFFITAGGDLSKPHYSNTKQSDFNYPIYSNAIEKKGLCG
CCCCCCEEECCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCHHHCCCCC
YSSFFIIKNKSITITARGTIGVAFFRDYPYVPIGRLLVLQPKISNIDCRFYAEYINSKVK
CCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEHHHHCCEEE
FNTEQTTIPQLTIPKVALCEIPLPPLNEQNAIANILSALDRYLCALDALILKKESVKKAL
ECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFELLSQKKRLKGFNQAWQRVRLGDIAEIKRGVRITKNELDVFGKYPVVSGGVGFLGYTN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCHHHHEECCCEEECCCEEEEECC
NFNRYENTITIAQYGTAGYVNFQKNKFWANDVCFCIYPNKDIIKNIFLYYFLKVNQNYLY
CCCCCCCEEEEEECCCEEEEEEECCCEECCCEEEEEECCHHHHHHHHHHEEEEECCCEEE
EISNRNATPYSISKDKILDFEILLPPLNEQIAIANILSDLDNEIASLKNKKRQFENIKKA
EEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNHDLMSAKIRVLKK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]