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The map label for this gene is ispE [H]

Identifier: 188528213

GI number: 188528213

Start: 1434627

End: 1435430

Strand: Direct

Name: ispE [H]

Synonym: HPSH_07385

Alternate gene names: 188528213

Gene position: 1434627-1435430 (Clockwise)

Preceding gene: 188528212

Following gene: 188528214

Centisome position: 89.19

GC content: 40.05

Gene sequence:

>804_bases
ATGCATGCCTTTGAAGTTTATCCTAAAGTCAATATTTTTTTAAAAATCCTTCACAAAGAAGGGGCTTATCATAAGCTCAT
TTCTCGCATGTGTTTGGTCAAAGATAAATTCAAAGATATTATCAGCGTCAAAAGCGCGCTTTCCTTTTCGCTAAAAGGGG
ATTTTGACTGCCCTTTAGAAGAAAACTCGCTCTTTAAAGCCCTCCAAATTTTAAAAAATTTTTTAAAATCAAAAAATTTC
TCTCATTCTGCCATCAAATCCCTAGACACCCTAGCGATTGAAGTGGAAAAAAACATCCCCACTCAAGCCGGGTTAGGCGG
TGGGAGCGCTGATGCTGGGGGGCTATTGTATCATTTGAATCAAATTTTTGACTGGCGTTTGAGTTTAGAAGAGCTTTATA
GCATGGGATCTTTAGTGGGCGCAGACACCAACTTTTTCATCTCGCAACACAAAAGCGCTAACGCCACTTCTTATGGCGAA
GTTATTGAAAATTTTGAAGAAGAGCCTTTAGAAAATCGCCTAGAAATCTATGCACCAAACCATGTTTTTTGCAGCACCAA
AGCCATTTATCAAGCTTATAAGCCTGAAACTTGTTTTTCTCAAGCTAAAGAGTGGCTTAAAAAACCGAGTTTAGAATGCC
TAAAGACTTGCGACAGAAACGGATTAAACGATCTTTTAAAGCCGGCTTTACTCACTAACCAAGCCTTAAGAGGTATAGAA
AGCGAACTGGGTAAGGAGTGGTTTTTTAGCGGGAGCGGGAGCGCGTTTTTTAGGCTAAAGCCTGCGTTAAAAGGGGGCGA
ATGA

Upstream 100 bases:

>100_bases
GCGCTGAACTCAAAGAGGCCATTGTGAGCGAAAACCAAAAAGCTTCTGCGTCCGTAGATGAAAGCTTGTTAGAAAACATC
AAAAAGGTCATTAAGCCTTG

Downstream 100 bases:

>100_bases
AACTCATTGCCAGCAATAAAAAAGCCTATTTTGACTATGAAATTTTGGAAACTTTAGAAGCGGGATTAGCGCTTTTAGGC
TCTGAAGTGAAGGCTTTGAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MHAFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKFKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKNF
SHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNQIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGE
VIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQAKEWLKKPSLECLKTCDRNGLNDLLKPALLTNQALRGIE
SELGKEWFFSGSGSAFFRLKPALKGGE

Sequences:

>Translated_267_residues
MHAFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKFKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKNF
SHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNQIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGE
VIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQAKEWLKKPSLECLKTCDRNGLNDLLKPALLTNQALRGIE
SELGKEWFFSGSGSAFFRLKPALKGGE
>Mature_267_residues
MHAFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKFKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKNF
SHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNQIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGE
VIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQAKEWLKKPSLECLKTCDRNGLNDLLKPALLTNQALRGIE
SELGKEWFFSGSGSAFFRLKPALKGGE

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 29932; Mature: 29932

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKFKDIISVKSALSFSLKGDFDCPLE
CCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ENSLFKALQILKNFLKSKNFSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLN
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHH
QIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGEVIENFEEEPLENRLEIYAPN
HHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCHHHCCEEEECCC
HVFCSTKAIYQAYKPETCFSQAKEWLKKPSLECLKTCDRNGLNDLLKPALLTNQALRGIE
CEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SELGKEWFFSGSGSAFFRLKPALKGGE
HHHCHHHEECCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MHAFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKFKDIISVKSALSFSLKGDFDCPLE
CCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ENSLFKALQILKNFLKSKNFSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLN
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHH
QIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGEVIENFEEEPLENRLEIYAPN
HHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCHHHCCEEEECCC
HVFCSTKAIYQAYKPETCFSQAKEWLKKPSLECLKTCDRNGLNDLLKPALLTNQALRGIE
CEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SELGKEWFFSGSGSAFFRLKPALKGGE
HHHCHHHEECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA