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The map label for this gene is smpB

Identifier: 188528214

GI number: 188528214

Start: 1435427

End: 1435885

Strand: Direct

Name: smpB

Synonym: HPSH_07390

Alternate gene names: 188528214

Gene position: 1435427-1435885 (Clockwise)

Preceding gene: 188528213

Following gene: 188528215

Centisome position: 89.24

GC content: 32.9

Gene sequence:

>459_bases
ATGAAACTCATTGCCAGCAATAAAAAAGCCTATTTTGACTATGAAATTTTGGAAACTTTAGAAGCGGGATTAGCGCTTTT
AGGCTCTGAAGTGAAGGCTTTGAGGCAAACTAGGGTGAATTTAAAAGATAATTTTGTTAAAATAATCAAAGGGGAAGCTT
TTTTATTTGGTGTTCATATATCATATTTAGATACAATCCATGCGTATTACAAGCCCAATGAAAGGCGAGAGCGTAAGTTG
CTTTTACACAAAAAGCAATTATTGAAATGGCAGATTGAGGCGTCTAAAGAACGCTTGAGTATCGTAGGATTGAAATTGTA
TTTTAACCAAAGGAATAAAGCTAAAATCCAAATTGCTTTAGTGAAAGGAAAAAAATTGCACGATAAAAGACAAAGTCTTA
AGGAAAAAGCGCTCAATAAAGAAATTCTTGCTGATTTAAAACACCACTTTAAAGGATAA

Upstream 100 bases:

>100_bases
AAGCCTTAAGAGGTATAGAAAGCGAACTGGGTAAGGAGTGGTTTTTTAGCGGGAGCGGGAGCGCGTTTTTTAGGCTAAAG
CCTGCGTTAAAAGGGGGCGA

Downstream 100 bases:

>100_bases
TAATGAAAGAAATGGTAGATTATGGGGTTATAGGGTTTTTGATCTTTTTATCAGTCATCGTTATAGCGATCGCAATAGAA
AGGTTGTGGTTTTTTGCCAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKLIASNKKAYFDYEILETLEAGLALLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNKAKIQIALVKGKKLHDKRQSLKEKALNKEILADLKHHFKG

Sequences:

>Translated_152_residues
MKLIASNKKAYFDYEILETLEAGLALLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNKAKIQIALVKGKKLHDKRQSLKEKALNKEILADLKHHFKG
>Mature_152_residues
MKLIASNKKAYFDYEILETLEAGLALLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNKAKIQIALVKGKKLHDKRQSLKEKALNKEILADLKHHFKG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=133, Percent_Identity=41.3533834586466, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17768; Mature: 17768

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIASNKKAYFDYEILETLEAGLALLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHI
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEHH
SYLDTIHAYYKPNERRERKLLLHKKQLLKWQIEASKERLSIVGLKLYFNQRNKAKIQIAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCEEEEEEE
VKGKKLHDKRQSLKEKALNKEILADLKHHFKG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIASNKKAYFDYEILETLEAGLALLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHI
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEHH
SYLDTIHAYYKPNERRERKLLLHKKQLLKWQIEASKERLSIVGLKLYFNQRNKAKIQIAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCEEEEEEE
VKGKKLHDKRQSLKEKALNKEILADLKHHFKG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA