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The map label for this gene is ksgA

Identifier: 188528194

GI number: 188528194

Start: 1422109

End: 1422924

Strand: Reverse

Name: ksgA

Synonym: HPSH_07290

Alternate gene names: 188528194

Gene position: 1422924-1422109 (Counterclockwise)

Preceding gene: 188528196

Following gene: 188528193

Centisome position: 88.46

GC content: 38.36

Gene sequence:

>816_bases
ATGGTAGTAGCTAAAAAGTCTTTAGGACAGCATTTTTTAACGGACGAGTCGTTTTTAGACAGAATTGTTGATGCTTTGCC
CCCCTTAAACCCATTGAAATTAATTGAAATTGGCGTGGGGCTAGGGGATTTGACTCTTAAGTTGTTAGATCACTATCCTT
TAAAGACTTATGAGATAGATAGCAGTTTGTGCGAGAAAATGCGATCAAAACTAAAGGCGCAAAAAAAGCCTTTTGCATTA
GAATTAGTGGAAAAAGACGCTCTTTTTTTAAAAGAAGAAGAGCCTTATTTTTTGATCTCTAATTTGCCTTATTATATCGC
TACTAGGCTTGTTTTAAACGCACTCAAAGACCCTAAATGCAGGGGTTTATTGGTGATGGCACAAAAGGAAGTGGCGCTCA
AATTTTGCGCTAAAGATTCACAGAACGCCTTAAGCGTTTTAACTCAAGCAATAGGGAATGCTACCCTTTTGTTTGATGTG
CCGCCTAGCGCGTTTAGCCCGCCTCCAAAGGTGTTTTCTAGCGTGTTTGAAGTGATTAAAGAGCCGCTGAAAGAAAAGGC
GTTAGCTTCATTACTCCAAGCGCCATTTTTTGAAGAAGCCCTACAAAAAGGGTTTGAAACATTAGAAGATTTTTTGAAAG
CCTGTTTCTCATCTCCTAGGAAGACGCTTTCAAACAATCTTAAAAAAAGCGTTTCTTATAGAGAAAAGCTTGATAAGGTG
TTAGATTTTTTAGCGTTAGAAAACCAACCAACAAGCGTGAGGGCGTCTGAGATAAAAGATTATCTCAAGCTCTTAGAATA
CCTTTTAAAAGGCTAA

Upstream 100 bases:

>100_bases
TCTTTGAATGAAAAAAGCTATAATAAAGAAGTTGCATTAAAAAGGAAAGACAAAATCCCTTTTTAAGGTATAATATGAGG
AGTTTTTATAAGCGCTTCTT

Downstream 100 bases:

>100_bases
TGCCTTTTTAAAAAATTGAAAGGAATAATCGTTATGGCTGATAACAACCATTATGAAAACAATGAAATCAATGAAAACAA
TGAAAACAGCAGTGAAAATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVVAKKSLGQHFLTDESFLDRIVDALPPLNPLKLIEIGVGLGDLTLKLLDHYPLKTYEIDSSLCEKMRSKLKAQKKPFAL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNALKDPKCRGLLVMAQKEVALKFCAKDSQNALSVLTQAIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLLQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLEYLLKG

Sequences:

>Translated_271_residues
MVVAKKSLGQHFLTDESFLDRIVDALPPLNPLKLIEIGVGLGDLTLKLLDHYPLKTYEIDSSLCEKMRSKLKAQKKPFAL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNALKDPKCRGLLVMAQKEVALKFCAKDSQNALSVLTQAIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLLQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLEYLLKG
>Mature_271_residues
MVVAKKSLGQHFLTDESFLDRIVDALPPLNPLKLIEIGVGLGDLTLKLLDHYPLKTYEIDSSLCEKMRSKLKAQKKPFAL
ELVEKDALFLKEEEPYFLISNLPYYIATRLVLNALKDPKCRGLLVMAQKEVALKFCAKDSQNALSVLTQAIGNATLLFDV
PPSAFSPPPKVFSSVFEVIKEPLKEKALASLLQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
LDFLALENQPTSVRASEIKDYLKLLEYLLKG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=277, Percent_Identity=25.6317689530686, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=24.2957746478873, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786236, Length=290, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25146882, Length=287, Percent_Identity=26.1324041811847, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324989, Length=265, Percent_Identity=27.9245283018868, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21358017, Length=181, Percent_Identity=30.939226519337, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30487; Mature: 30487

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVDALPPLNPLKLIEIGVGLGDLTLKLLDHYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEECC
SSLCEKMRSKLKAQKKPFALELVEKDALFLKEEEPYFLISNLPYYIATRLVLNALKDPKC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHCCCCC
RGLLVMAQKEVALKFCAKDSQNALSVLTQAIGNATLLFDVPPSAFSPPPKVFSSVFEVIK
CCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLLQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIKDYLKLLEYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVAKKSLGQHFLTDESFLDRIVDALPPLNPLKLIEIGVGLGDLTLKLLDHYPLKTYEID
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEECC
SSLCEKMRSKLKAQKKPFALELVEKDALFLKEEEPYFLISNLPYYIATRLVLNALKDPKC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHCCCCC
RGLLVMAQKEVALKFCAKDSQNALSVLTQAIGNATLLFDVPPSAFSPPPKVFSSVFEVIK
CCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EPLKEKALASLLQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKSVSYREKLDKV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LDFLALENQPTSVRASEIKDYLKLLEYLLKG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA