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The map label for this gene is dadA [C]

Identifier: 188527766

GI number: 188527766

Start: 985550

End: 986782

Strand: Reverse

Name: dadA [C]

Synonym: HPSH_04965

Alternate gene names: 188527766

Gene position: 986782-985550 (Counterclockwise)

Preceding gene: 188527767

Following gene: 188527765

Centisome position: 61.35

GC content: 40.71

Gene sequence:

>1233_bases
ATGAAAAAAGAGGTCGTGGTAATAGGCGGTGGGATTGTAGGGCTTTCTTGTGCGTATTCTATGCATAAATTAGGGCATAA
GGTTTGCGTGATTGAAAAAAGCGATGGCACAAACGGCACTTCTTTTGGGAATGCCGGGCTCATTTCTGCGTTTAAAAAAG
CCCCGCTCTCATGCCCTGGTGTGGTGTTAGACACCCTAAAACTCATGCTCAAAAACCAAGCCCCTTTAAAATTCCATTTT
GGGCTTAATTTAAAGCTTTATCAATGGATTTTAAAGTTTATGCAAAGTGCGAACGCCAAATCCACGCGTCGCACCATGGC
GTTGTTTGAACGCTACGGGTGGCTGAGTGTTGATATGTATCATCAAATGCTAAAAGACGGCATGGACTTTTGGTATAAAG
AAGACGGGCTTTTAATGATCTACACTTTAGAAGAAAGTTTTGAAAAAAAGCTTAAAACTTGCGATAACAGCGGTGCTTAT
AAGATCTTGAATGTGAAAGAAACCAAAGAATACATGCCTATTGTTAATGACAATATCTGCGGGAGCGTGCTTTTAACCGA
AAACGCGCATGTGGATCCGGGCGAAGTGATGCGCTCTTTGCAAGAATATTTACAAAATGCGGGCGTGGAGTTTCTTTATA
ATGAAGAAGTGGTTGATTTTGAGTTTAAAAATAACCTCATTGATGGCGTTATCACGCATAAGGAAAAAATCCAAGCAGAA
AGAATCATTCTAGCCACCGGGGCTAACCCCGCTCTCATTAAAAAAACCAAGAACGATTTTTTAATGATGGGGGCTAAAGG
CTATAGCATCACTTTTAAAATGCCTGAAGAATTAAAGCCCAAAACCTCTTCTTTATTTGCGGATATTTTCATGGCAATGA
CCCCACGAAGAGACACCGTTAGGATCACTTCTAAATTAGAATTAAACACCAACAACGCCCTCATTGATAAAGAGCAAATC
TCTAACATGAAAAAGAATTTAGCCGCTTTCACGCAGCCTTTTGAAATGAAAGACGCCATAGAGTGGTGCGGTTTCAGGCC
CTTAACCCCTAATGATATTCCTTATTTAGGGTTTGACAAACGCTATAAAAACTTAATCCATGCCACAGGGCTAGGGTGGC
TTGGCATCACTTTTGGCCCGGCCATTGGTAAAATCATCGCCGATTTAAGCCAAGACGGATCGAATGAAAAAAATGCCGAT
ATTATGCTTTTTTCTGCATTTTTTAGGGATTAA

Upstream 100 bases:

>100_bases
CCGGCCAGAGCGACCTTTCAAGTGGCTAAACTGCCTAAAGACGCTTTAGTAGAAATTGAAGCGATAGCCATTAAGTAATT
TTATTCAAGGGATTATCAGC

Downstream 100 bases:

>100_bases
GGAATTTCTTTTTTAAACCCTAGTTTATTAAGGAGTTTTTATGGAAACGATTGATTCGGTGGTGCGTTTGTTATCTAATT
TTGTGTGGGGGATTCCCATG

Product: D-Amino acid dehydrogenase

Products: 2-oxo acid; NH3; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGTNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMQSANAKSTRRTMALFERYGWLSVDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILNVKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVVDFEFKNNLIDGVITHKEKIQAE
RIILATGANPALIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
SNMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGFDKRYKNLIHATGLGWLGITFGPAIGKIIADLSQDGSNEKNAD
IMLFSAFFRD

Sequences:

>Translated_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGTNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMQSANAKSTRRTMALFERYGWLSVDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILNVKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVVDFEFKNNLIDGVITHKEKIQAE
RIILATGANPALIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
SNMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGFDKRYKNLIHATGLGWLGITFGPAIGKIIADLSQDGSNEKNAD
IMLFSAFFRD
>Mature_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGTNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMQSANAKSTRRTMALFERYGWLSVDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILNVKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVVDFEFKNNLIDGVITHKEKIQAE
RIILATGANPALIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
SNMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGFDKRYKNLIHATGLGWLGITFGPAIGKIIADLSQDGSNEKNAD
IMLFSAFFRD

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=400, Percent_Identity=26, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1

Molecular weight: Translated: 46227; Mature: 46227

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGTNGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFMQSANAKSTRRTMALFERYGWLSVDMY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAYKILNVKETKEYMPIVNDNIC
HHHHHCCHHHEECCCCEEEEEECHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCCC
GSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVVDFEFKNNLIDGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHCCCHHHHHHHHHHHCCC
RIILATGANPALIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCCEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQISNMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGFDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIADLSQDGSNEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGTNGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFMQSANAKSTRRTMALFERYGWLSVDMY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAYKILNVKETKEYMPIVNDNIC
HHHHHCCHHHEECCCCEEEEEECHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCCC
GSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVVDFEFKNNLIDGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHCCCHHHHHHHHHHHCCC
RIILATGANPALIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCCEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQISNMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGFDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIADLSQDGSNEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-amino acid; H2O; acceptor

Specific reaction: a D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]