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The map label for this gene is dnaN [H]

Identifier: 188527661

GI number: 188527661

Start: 863233

End: 864357

Strand: Reverse

Name: dnaN [H]

Synonym: HPSH_04415

Alternate gene names: 188527661

Gene position: 864357-863233 (Counterclockwise)

Preceding gene: 188527662

Following gene: 188527660

Centisome position: 53.74

GC content: 36.0

Gene sequence:

>1125_bases
ATGAAAATCAGTGTTAGTAAAAACGATTTAGAAAATACTTTGCGCTACTTGCAAGCTTTTTTGGATAAAAAGGACGCTTC
TTCTATCGCTTCACACATCCATTTAGAAGTCATTAAAGATAAGCTTTTTTTGAAAGCTAGTGATTCAGATATTGGGCTAA
AAAGCTATATTTTTACGCAATCTAGCGATAAAGAGGGCGTTGGCACGATCAATGGGAAAAAGTTTTTGGATATTATCTCA
TGTTTAAAAGACTCCAATATTATTTTAGAGACTAAAGATGACAGCTTGGTGATCAAACAAAATAAAAGCTCTTTCAAACT
CCCCATGTTTGACGCTGATGAGTTCCCTGAATTCCCTGTTATTGAGCCAAAAGTGAGTTTAGAAGTCAATGCCCCCTTTT
TAACAGATGCGTTTAAAAAGATCGCTCCTGTGATTGAGCAAACCAGCCATAAAAGGGAATTAGCCGGTATTTTAATGCAA
TTCAATCAAAAACATCAAACCCTTTCAGTGGTAGGCACGGATACCAAACGGCTCTCTTACACGCAGTTAGAAAAAATCTC
TATCCATTCCACTGAAGAAGACATCTCTTGCATTTTACCTAAAAGAGCTTTATTAGAAATCCTTAAGCTTTTTTATGAAA
ATTTCAGTTTTAAAAGCGATGGCATGTTAGCGGTGGTTGAAAACGAAATGCACACTTTTTTCACCAAGCTCATTGATGGG
AATTACCCTGATTATCAAAAAATCCTCCCTAAAGAATACATTTCTTCTTTCACTTTGGGCAAGGAAGAATTTAAAGAGAG
CATTAAGCTGTGCAGTTCTTTAAGCTCCACCATTAAACTCACTTTAGAAAAAAACAACGCTTTATTTGAATCTTTGGATT
CTGAACATAGCGAAACGGCTAAAACCTCTGTTGAGATTGAAAAAGGTTTGGATATTGAAAAAGCCTTTCATTTGGGCGTG
AACGCGAAATTTTTCCTTGAAGCCTTAAACGCTTTAGGGACAACGCAATTTGTTTTAAAATGCAATGAGCCTTCTTCGCC
TTTTTTGATCCAAGAGTCTCTTGATGAAAAGCAAAGCCATTTGAACGCTAAAATTTCCACTTTGATGATGCCAATCACAC
TATAA

Upstream 100 bases:

>100_bases
TTTTTCCAATCGTATAGGGGTAGGAATACGCTTAAACCCTTAAAAAAGCGTTCTTTTATGCTATAATTAAGACCAAAAAT
TCAAGGGGATTATTTTAGCT

Downstream 100 bases:

>100_bases
AGGCTGATTGAATGCAAAATTACCAGAGCCATAGTATCAAGGTTTTAAAAGGCTTAGAGGGGGTTAGGAAACGCCCTGGA
ATGTATATTGGCGATACCAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKISVSKNDLENTLRYLQAFLDKKDASSIASHIHLEVIKDKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIILETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSLEVNAPFLTDAFKKIAPVIEQTSHKRELAGILMQ
FNQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVVENEMHTFFTKLIDG
NYPDYQKILPKEYISSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLNAKISTLMMPITL

Sequences:

>Translated_374_residues
MKISVSKNDLENTLRYLQAFLDKKDASSIASHIHLEVIKDKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIILETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSLEVNAPFLTDAFKKIAPVIEQTSHKRELAGILMQ
FNQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVVENEMHTFFTKLIDG
NYPDYQKILPKEYISSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLNAKISTLMMPITL
>Mature_374_residues
MKISVSKNDLENTLRYLQAFLDKKDASSIASHIHLEVIKDKLFLKASDSDIGLKSYIFTQSSDKEGVGTINGKKFLDIIS
CLKDSNIILETKDDSLVIKQNKSSFKLPMFDADEFPEFPVIEPKVSLEVNAPFLTDAFKKIAPVIEQTSHKRELAGILMQ
FNQKHQTLSVVGTDTKRLSYTQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVVENEMHTFFTKLIDG
NYPDYQKILPKEYISSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETAKTSVEIEKGLDIEKAFHLGV
NAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSHLNAKISTLMMPITL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=342, Percent_Identity=21.3450292397661, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42202; Mature: 42202

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVSKNDLENTLRYLQAFLDKKDASSIASHIHLEVIKDKLFLKASDSDIGLKSYIFTQ
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEEC
SSDKEGVGTINGKKFLDIISCLKDSNIILETKDDSLVIKQNKSSFKLPMFDADEFPEFPV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
IEPKVSLEVNAPFLTDAFKKIAPVIEQTSHKRELAGILMQFNQKHQTLSVVGTDTKRLSY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCH
TQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVVENEMHTFFTKLIDG
HHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCC
NYPDYQKILPKEYISSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETA
CCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCHHHHHH
KTSVEIEKGLDIEKAFHLGVNAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSH
HHHEEHHHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHHH
LNAKISTLMMPITL
HHHHHHHEEEEECC
>Mature Secondary Structure
MKISVSKNDLENTLRYLQAFLDKKDASSIASHIHLEVIKDKLFLKASDSDIGLKSYIFTQ
CEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEEEEC
SSDKEGVGTINGKKFLDIISCLKDSNIILETKDDSLVIKQNKSSFKLPMFDADEFPEFPV
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
IEPKVSLEVNAPFLTDAFKKIAPVIEQTSHKRELAGILMQFNQKHQTLSVVGTDTKRLSY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCH
TQLEKISIHSTEEDISCILPKRALLEILKLFYENFSFKSDGMLAVVENEMHTFFTKLIDG
HHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCC
NYPDYQKILPKEYISSFTLGKEEFKESIKLCSSLSSTIKLTLEKNNALFESLDSEHSETA
CCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCHHHHHH
KTSVEIEKGLDIEKAFHLGVNAKFFLEALNALGTTQFVLKCNEPSSPFLIQESLDEKQSH
HHHEEHHHCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHHH
LNAKISTLMMPITL
HHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]