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The map label for this gene is pldA [C]

Identifier: 188527662

GI number: 188527662

Start: 864415

End: 865482

Strand: Reverse

Name: pldA [C]

Synonym: HPSH_04420

Alternate gene names: 188527662

Gene position: 865482-864415 (Counterclockwise)

Preceding gene: 188527663

Following gene: 188527661

Centisome position: 53.81

GC content: 40.26

Gene sequence:

>1068_bases
ATGAAAGGCATTTTGCTCTTTATGATTTTTGTAGTTTGTCAGTTAGAAGGCAAAAAATTTTCACAAGATAATTTTAAGGT
GGATTATAACTACTATTTGTGCAAACAGGATTTGCACATCATTAAAACGCAAAACGATTTGTCCAATGCCTGGTATCTCC
CTCCACAAAAAGCCCCCAAAGAACATTCTTGGGTGGATTTTGCTAAAAAATATTTAAACATGATGGATTATCTAGGCACT
TATTTTCTGCCTTTTTATCATAGTTTCACCCCCATTTTTCAATGGTACCACCCCAATATCAACCCGTATCAACGCAATGA
GTTTAAATTCCAGATTAGTTTTAGAGTGCCTGTATTTAGGCATATTCTTTGGACTAAAGGTACGCTTTATTTGGCTTACA
CCCAAACTAACTGGTTTCAAATTTACAATAACCCCCAATCCGCTCCCATGCGAATGATCAATTTCATGCCTGAACTCATT
TATGTTTATCCTATTAATTTTGAACCTTTTGGGGGTAAAATAGGGAAATTTTCTGAGATTTGGATAGGTTGGCAGCACAT
TTCTAATGGCGTGGGAGGCGCGCAATGTTACCAGCCTTTTAATAAAGAAGGCAATCCTGAAAACCAGTTTCCAGGACAAC
CTGTAATCGTTAAAGATTATAATGGGCAAAAAGATGTGCGCTGGGGGGGGTGTCGTTCGGTGAGCGCGGGGCAACGCCCT
GTGTTTCGTTTGGTGTGGGAAAAGGGAGGCTTGAAAATCATGGTCGCTTATTGGCCCTATGTCCCTTATGATCAATCCAA
CCCTAATTTGATTGATTACATGGGGTATGGTAACGCTAAAGTTGATTACAGGAGAGGGCGCCATCATTTTGAATTGCAAC
TTTATGATATTTTCACGCAATACTGGCGTTATGATCGCTGGCATGGGGCTATCCGCTTAGGCTATACCTACCGCATTAAC
CCTTTTGTGGGAATTTATGCGCAGTGGTTTAACGGCTATGGCGATGGCTTGTATGAATACGATGTTTTTTCCAATCGTAT
AGGGGTAGGAATACGCTTAAACCCTTAA

Upstream 100 bases:

>100_bases
CAAACGCTTTTAAAGCGTGGCATTTTAGCGTTCGTTTTATCGCGCCTGTAGTGATTTTAGCGATTTTCATCTTGCAATTT
AAGTGAAATAAGGATGCTTG

Downstream 100 bases:

>100_bases
AAAAGCGTTCTTTTATGCTATAATTAAGACCAAAAATTCAAGGGGATTATTTTAGCTATGAAAATCAGTGTTAGTAAAAA
CGATTTAGAAAATACTTTGC

Product: phospholipase A1

Products: 2-acylglycerophosphocholine; carboxylate

Alternate protein names: Outer Membrane Phospholipase A

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKGILLFMIFVVCQLEGKKFSQDNFKVDYNYYLCKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNNPQSAPMRMINFMPELI
YVYPINFEPFGGKIGKFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQYWRYDRWHGAIRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP

Sequences:

>Translated_355_residues
MKGILLFMIFVVCQLEGKKFSQDNFKVDYNYYLCKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNNPQSAPMRMINFMPELI
YVYPINFEPFGGKIGKFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQYWRYDRWHGAIRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
>Mature_355_residues
MKGILLFMIFVVCQLEGKKFSQDNFKVDYNYYLCKQDLHIIKTQNDLSNAWYLPPQKAPKEHSWVDFAKKYLNMMDYLGT
YFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQTNWFQIYNNPQSAPMRMINFMPELI
YVYPINFEPFGGKIGKFSEIWIGWQHISNGVGGAQCYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRP
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQYWRYDRWHGAIRLGYTYRIN
PFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP

Specific function: Hydrolysis Of Phosphatidylcholine With Phospholipase A2 (Ec 3.1.1.4) And Phospholipase A1 (Ec 3.1.1.32) Activities. Required For Efficient Secretion Of Bacteriocins; Seems To Be Dormant In Normal Growing Cells. [C]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Outer Membrane; One Of The Very Few Enzymes Located There [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI2367300, Length=251, Percent_Identity=27.0916334661355, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.32

Molecular weight: Translated: 42335; Mature: 42335

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGILLFMIFVVCQLEGKKFSQDNFKVDYNYYLCKQDLHIIKTQNDLSNAWYLPPQKAPK
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCC
EHSWVDFAKKYLNMMDYLGTYFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEEEEEECHHHH
HILWTKGTLYLAYTQTNWFQIYNNPQSAPMRMINFMPELIYVYPINFEPFGGKIGKFSEI
HHHHCCCEEEEEEEECCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHE
WIGWQHISNGVGGAQCYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRP
EEEHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQ
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEEEHHHHHHH
YWRYDRWHGAIRLGYTYRINPFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MKGILLFMIFVVCQLEGKKFSQDNFKVDYNYYLCKQDLHIIKTQNDLSNAWYLPPQKAPK
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCC
EHSWVDFAKKYLNMMDYLGTYFLPFYHSFTPIFQWYHPNINPYQRNEFKFQISFRVPVFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCEEEEEEEEECHHHH
HILWTKGTLYLAYTQTNWFQIYNNPQSAPMRMINFMPELIYVYPINFEPFGGKIGKFSEI
HHHHCCCEEEEEEEECCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHE
WIGWQHISNGVGGAQCYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRP
EEEHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
VFRLVWEKGGLKIMVAYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQ
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEEEHHHHHHH
YWRYDRWHGAIRLGYTYRINPFVGIYAQWFNGYGDGLYEYDVFSNRIGVGIRLNP
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphatidylcholine; H2O

Specific reaction: phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate

General reaction: Carboxylic ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA