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The map label for this gene is prrB [H]

Identifier: 188527366

GI number: 188527366

Start: 547884

End: 549158

Strand: Direct

Name: prrB [H]

Synonym: HPSH_02865

Alternate gene names: 188527366

Gene position: 547884-549158 (Clockwise)

Preceding gene: 188527365

Following gene: 188527370

Centisome position: 34.06

GC content: 35.69

Gene sequence:

>1275_bases
ATGCATAAAATAGAGCGCTTACTCCAAACTTTAGTGCCTAAGGGGGTGGAGTTTAAAACGCTTGAAGAGGTTTTTGAAAT
TAAAAATGGTTACACCCCATCAAAAAACAATCCTGAATTTTGGGAAAAAGGGACTATCCCTTGGTTTAGAATGGAAGACA
TTAGAGAAAATGGGAGGATTTTAAAAGACTCTATCCAACACATTACCCCAAAGGCTTTAAAGGGTAAGAAATTATTCCCT
AAAAATTCTATTATTATTTCTACAACGGCAACGATAGGAGAGCATGCCCTTTTAATCGTTGATTCGTTAGCGAATCAACA
ATTCACTTTTTTAAGCAAAAAAGCGAATTGTGATATTGCTTTAGACATGAAATTCTTTTTTTATCAATGTTTTCTTTTGG
GGGAATGGTGCAAAAAAAACACTAATGTTTCAGGTTTTGCTTCTATGGATATGACTGCTTTTAAAAAATATAAGTTCCCC
ATCCCACCCCTAGAAATCCAACAAGAGATCGTTAAGATTTTGGACGCTTTCACAGAATTAAACACAGAATTAAACACAGA
ATTAAAAGCGCGAAAAAAGCAATACCAGTATTACCAAAACATGCTTTTAGACTTTAAGGACATCCATTCAAACCATAAAG
ACGCAAAAATTAGCGCAAAAACCTACCCCAAACGCTTAAAAACCTTACTCCAAACCTTAGCGCCTAAGGGGGTGGAGTTT
AGGAAATTGGGGGAGGTGTGTGAAATTTTAGATAATCGGCGTATCCCAATTGCGAAAAATAAAAGAAATCCGGGAATTTA
TCCTTATTATGGAGCAAATGGAATTCAAGATTATATTGATAGCTATATTTTTGATGGGGATTTTGTGCTAGTTGGTGAAG
ATGGGAGCGTTATCAATAAGGATAATACTCCTGTTGTGAATTGGGCAAGCGGAAAAATATGGGTGAACAATCATGCTCAT
GTGCTTCAAACAAAAAATGAATTAAAATTAAAGTTTTTGTATTTTTATTTACAAACGATAGATGTTAGCTATTGTGTTGC
TGGAACTCCGCCAAAAATCAACCAAGAAAATTTAAAACAAATAACAATCCCCATCCCACCCCTAGAAATCCAACAAGAGA
TCGTTAAGATTTTGGATCAATTCTTGGCCCTAACCACCGATTTATTAGCCGGTATCCCGGCTGAAATAGAAGCCAGGAAA
AAGCAATACCAATATTACAGAGAAAAACTACTGACCTTCAAACCACTAACCCCCCACAAAGAAGTTGAAAAATGA

Upstream 100 bases:

>100_bases
TCTTTGGCGATGTGGGGGTTACGCTTTTAGCCTTAGCCAACTCCATGCGCGCGATGAGGGCTTAATGATCTTATTATCAG
CCATTTAGAAGGGGGGCAAA

Downstream 100 bases:

>100_bases
GTTTTAAAATGACAAAACAAAGCAATTAAAAGCCCATTTTTAAAAATAAAAGTTTTAGTATAGCAGAGTCAAAAATAGCG
TAATAAAAAAATTTAAAAAT

Product: anti-codon nuclease masking agent (prrB)

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPKGVEF
RKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIWVNNHAH
VLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARK
KQYQYYREKLLTFKPLTPHKEVEK

Sequences:

>Translated_424_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPKGVEF
RKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIWVNNHAH
VLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARK
KQYQYYREKLLTFKPLTPHKEVEK
>Mature_424_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPKGVEF
RKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINKDNTPVVNWASGKIWVNNHAH
VLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARK
KQYQYYREKLLTFKPLTPHKEVEK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49067; Mature: 49067

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCC
LKDSIQHITPKALKGKKLFPKNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEECCCCCCCEE
LDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EEHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPKGVEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCH
RKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINK
HHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHEECCCEEEECCCCCEECC
DNTPVVNWASGKIWVNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKQ
CCCCEEEECCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCE
ITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARKKQYQYYREKLLTFKPLTPHK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVEK
HCCC
>Mature Secondary Structure
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCC
LKDSIQHITPKALKGKKLFPKNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEECCCCCCCEE
LDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EEHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPKGVEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCH
RKLGEVCEILDNRRIPIAKNKRNPGIYPYYGANGIQDYIDSYIFDGDFVLVGEDGSVINK
HHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHEECCCEEEECCCCCEECC
DNTPVVNWASGKIWVNNHAHVLQTKNELKLKFLYFYLQTIDVSYCVAGTPPKINQENLKQ
CCCCEEEECCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCE
ITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARKKQYQYYREKLLTFKPLTPHK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVEK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]