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The map label for this gene is wcaE [C]

Identifier: 188526907

GI number: 188526907

Start: 95317

End: 96099

Strand: Reverse

Name: wcaE [C]

Synonym: HPSH_00510

Alternate gene names: 188526907

Gene position: 96099-95317 (Counterclockwise)

Preceding gene: 188526908

Following gene: 188526903

Centisome position: 5.97

GC content: 38.31

Gene sequence:

>783_bases
TTGTTAAAAGTTTCTGTGATCACGGCGTGTTTTAATAGCGAAAAAACCATTGAAGACACCATTCTTTCCGTGCTTAATCA
AACTTATAAAAACATTGAATACATCATTATAGACGGGGCTAGCACGGATGGCACTTTAGAAATCATTCAAAAATACAAAG
ATAAAATCGCTTGCGCAATGAGTGAAAAAGATGAGGGCATTTATGACGCTATGAATAAAGGCATAAAGCGTTCTAGTGGG
GATATTATCGCTTTATTAAATAGCGATGATTTTTACAAAGATGAGTTTGTGGTAGAAAAAGTGGTGCATGAGTTTGAAAA
CAAAAATTGCGATAGCGTGTATGCGGATCTGGTGTTTGTCAAGCCTGATTGTTTAGAAAAAGTGGTCCGTTATTATGAAA
GCGGGGAGTTTGACCCTAAAACCTTGCTTTATGGCGTGGTGCCAGCGCACCCCACGCTTTTTGTCAAAAAGGCCATTTAT
GAACGCTATGGGCTCTACAAAACCGACTATAAGATTTCAGCGGATTTTGAGATGATCATCCGCTTGTTTGTGGTGCAAAA
AATAAGCTTTTCTTATTTGAAAGAAGTGTTAGTCATCATGCGCACCGGTGGGGTTAGCGCAAGGGGGTTTAAAAGCCTTT
TATTAAGGAATAAAGAAAATTTGAGAGCGTGTCAAGAAAACGGCATTCAAGCGAATGTTTTTTCCATGCTTTTAAAATAC
CCCAGAAAGATCATGGGCTTATTTAAAAGGGGATCTAAAGGGAGTCTAAAACGAAACGATTAA

Upstream 100 bases:

>100_bases
AAACATCGCTACGCATGTGAGCGAAACGACCATGAATTTAGACAAACAAGTGAATTTGTTTAAAACTTAATCAGGAGGAG
TTGATTAAAAAAGGGTTGGA

Downstream 100 bases:

>100_bases
AGAAAGATTAGCGTTAAATCGTGGTTTGAATCTTTCTTGATGGTTATTGTAGGTGTAAAAAACATTCCTCACTAAAGGCA
TTTCAATCCCAAACTGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASTDGTLEIIQKYKDKIACAMSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPDCLEKVVRYYESGEFDPKTLLYGVVPAHPTLFVKKAIY
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVIMRTGGVSARGFKSLLLRNKENLRACQENGIQANVFSMLLKY
PRKIMGLFKRGSKGSLKRND

Sequences:

>Translated_260_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASTDGTLEIIQKYKDKIACAMSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPDCLEKVVRYYESGEFDPKTLLYGVVPAHPTLFVKKAIY
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVIMRTGGVSARGFKSLLLRNKENLRACQENGIQANVFSMLLKY
PRKIMGLFKRGSKGSLKRND
>Mature_260_residues
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASTDGTLEIIQKYKDKIACAMSEKDEGIYDAMNKGIKRSSG
DIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFVKPDCLEKVVRYYESGEFDPKTLLYGVVPAHPTLFVKKAIY
ERYGLYKTDYKISADFEMIIRLFVVQKISFSYLKEVLVIMRTGGVSARGFKSLLLRNKENLRACQENGIQANVFSMLLKY
PRKIMGLFKRGSKGSLKRND

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788368, Length=216, Percent_Identity=28.2407407407407, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 29703; Mature: 29703

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASTDGTLEIIQKYKDKIACAM
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEKDEGIYDAMNKGIKRSSGDIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFV
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEE
KPDCLEKVVRYYESGEFDPKTLLYGVVPAHPTLFVKKAIYERYGLYKTDYKISADFEMII
CHHHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHHHHHHHHHHCCEECCCEECCCHHHHH
RLFVVQKISFSYLKEVLVIMRTGGVSARGFKSLLLRNKENLRACQENGIQANVFSMLLKY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
PRKIMGLFKRGSKGSLKRND
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLKVSVITACFNSEKTIEDTILSVLNQTYKNIEYIIIDGASTDGTLEIIQKYKDKIACAM
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
SEKDEGIYDAMNKGIKRSSGDIIALLNSDDFYKDEFVVEKVVHEFENKNCDSVYADLVFV
CCCCCCHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEE
KPDCLEKVVRYYESGEFDPKTLLYGVVPAHPTLFVKKAIYERYGLYKTDYKISADFEMII
CHHHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHHHHHHHHHHCCEECCCEECCCHHHHH
RLFVVQKISFSYLKEVLVIMRTGGVSARGFKSLLLRNKENLRACQENGIQANVFSMLLKY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
PRKIMGLFKRGSKGSLKRND
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]