BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is fadB1 [H]

Identifier: 187930182

GI number: 187930182

Start: 3264124

End: 3264900

Strand: Reverse

Name: fadB1 [H]

Synonym: Rpic_3114

Alternate gene names: 187930182

Gene position: 3264900-3264124 (Counterclockwise)

Preceding gene: 187930186

Following gene: 187930180

Centisome position: 82.81

GC content: 62.55

Gene sequence:

>777_bases
ATGTCCTACGAAAACATTCTGGTCGAAACGCGCGGTCGCGTCGGTCTCATTACGCTCAATCGTCCGAAGGCACTCAACGC
CCTCAACGATGCGCTCATGGACGAACTCGGTGGCGCGTTGCTCGCCTTCGATGCCGACGCCAACATTGGCGCCATCGTCA
TTACCGGCAGTGAAAAGGCGTTCGCCGCCGGCGCGGATATCGGCGCCATGGCCGATTACGACTTCGCTACCGTCTACAAG
AACGAATACATCACGCGCAACTGGGAAACCATTCGCCGCATCCGCAAGCCGGTGATCGCCGCGGTGGCCGGCTACGCGCT
CGGTGGCGGCTGTGAACTGGCCATGATGTGCGACATCATCCTCGCCGCCGATACCGCCAAGTTCGGCCAGCCGGAAATCA
AGCTCGGCACCATGCCCGGCGCAGGCGGCACGCAACGCCTGCCGCGCGCCGTCTCCAAGGCCAAGGCCATGGACCTCTGC
TTGACTGCCCGCATGATGGGCGCGGAGGAAGCCGAGCGCGCCGGCCTAGTCTCGCGCGTCATCCCCGCCGACAAGCTGCT
CGACGAAGCCATTCAGGCCGCAGAAACCATTGCCGGCTTCTCGCTGCCCACGGTCATGATGATCAAAGAGTCGATCAACG
CGGCCTACGAGACTTCGCTCAGCGAGGGCGTGCATCTGGAGCGCCGCCTGTTCCACGCCACATTCGCTACAGAAGACCAG
AAAGAAGGCATGCGCGCCTTCGTCGAAAAGCGTGCAGCGGCGTTCAAGCACCAATAA

Upstream 100 bases:

>100_bases
ATGGTCGGGCTGGTCATCTTGTCTCCTCCTCGGTCATCGGTTCATTGATTAACCGACCGGTTGGTCGGCTAATATTACAT
CCATTCCAACTGGAGCTTCC

Downstream 100 bases:

>100_bases
GGACGGCGTAGCAGGGTGACTTCGGGAATACCCGAGTAACGGTATCGGGTATCCCAGGCCTGCTTTTGCGAGTCCTTTTG
GGGCCGCAATCCCATATGCA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGGALLAFDADANIGAIVITGSEKAFAAGADIGAMADYDFATVYK
NEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTAKFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMGAEEAERAGLVSRVIPADKLLDEAIQAAETIAGFSLPTVMMIKESINAAYETSLSEGVHLERRLFHATFATEDQ
KEGMRAFVEKRAAAFKHQ

Sequences:

>Translated_258_residues
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGGALLAFDADANIGAIVITGSEKAFAAGADIGAMADYDFATVYK
NEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTAKFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLC
LTARMMGAEEAERAGLVSRVIPADKLLDEAIQAAETIAGFSLPTVMMIKESINAAYETSLSEGVHLERRLFHATFATEDQ
KEGMRAFVEKRAAAFKHQ
>Mature_257_residues
SYENILVETRGRVGLITLNRPKALNALNDALMDELGGALLAFDADANIGAIVITGSEKAFAAGADIGAMADYDFATVYKN
EYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDIILAADTAKFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCL
TARMMGAEEAERAGLVSRVIPADKLLDEAIQAAETIAGFSLPTVMMIKESINAAYETSLSEGVHLERRLFHATFATEDQK
EGMRAFVEKRAAAFKHQ

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=57.3076923076923, Blast_Score=301, Evalue=3e-82,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=34.2105263157895, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=31.0483870967742, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=36.046511627907, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI68989263, Length=239, Percent_Identity=33.8912133891213, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=34.2696629213483, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=30.188679245283, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=30.188679245283, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=30.7377049180328, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=24.7863247863248, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=24.7863247863248, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=22.9885057471264, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=22.9885057471264, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45643119, Length=244, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=244, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=22.6923076923077, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=22.6923076923077, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=25.1184834123223, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=25.1184834123223, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221307494, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136756, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221136753, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=45.8167330677291, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=36.4341085271318, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=34.8484848484849, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=34.0996168582375, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=30.0380228136882, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=32.0652173913043, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=31.1004784688995, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=56.1752988047809, Blast_Score=287, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=56.6115702479339, Blast_Score=276, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=32.2709163346614, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.4894514767932, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=32.2869955156951, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=36.6666666666667, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=216, Percent_Identity=36.1111111111111, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=216, Percent_Identity=36.1111111111111, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=216, Percent_Identity=36.1111111111111, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=29.0441176470588, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=223, Percent_Identity=29.1479820627803, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=32.48730964467, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=32.48730964467, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=30.0970873786408, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=25.2873563218391, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=235, Percent_Identity=26.3829787234043, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=58.4615384615385, Blast_Score=307, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=58.4615384615385, Blast_Score=307, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=35.9848484848485, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=31.2252964426877, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=28.4584980237154, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=31.1320754716981, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=32.2869955156951, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=28.8770053475936, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19921000, Length=188, Percent_Identity=32.9787234042553, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583077, Length=188, Percent_Identity=32.9787234042553, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583079, Length=188, Percent_Identity=32.9787234042553, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28571730, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27705; Mature: 27574

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYENILVETRGRVGLITLNRPKALNALNDALMDELGGALLAFDADANIGAIVITGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCC
FAAGADIGAMADYDFATVYKNEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDII
EECCCCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAADTAKFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMGAEEAERAGLVSRV
HHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
IPADKLLDEAIQAAETIAGFSLPTVMMIKESINAAYETSLSEGVHLERRLFHATFATEDQ
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
KEGMRAFVEKRAAAFKHQ
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYENILVETRGRVGLITLNRPKALNALNDALMDELGGALLAFDADANIGAIVITGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCC
FAAGADIGAMADYDFATVYKNEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCDII
EECCCCCCCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAADTAKFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMGAEEAERAGLVSRV
HHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
IPADKLLDEAIQAAETIAGFSLPTVMMIKESINAAYETSLSEGVHLERRLFHATFATEDQ
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHH
KEGMRAFVEKRAAAFKHQ
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]