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The map label for this gene is hupB [H]

Identifier: 187928947

GI number: 187928947

Start: 1965148

End: 1965420

Strand: Reverse

Name: hupB [H]

Synonym: Rpic_1866

Alternate gene names: 187928947

Gene position: 1965420-1965148 (Counterclockwise)

Preceding gene: 187928948

Following gene: 187928946

Centisome position: 49.85

GC content: 57.14

Gene sequence:

>273_bases
ATGAACAAAACAGATCTCATCGACCATGTGGCGACGCAAACTGACATGTCAAAAGCCGCGGCGGGTCGCGCCATCGACGC
ACTGATCGACGGCATCAAGGATGCGTTGTGCGAGGGCGGGTCGGTCACGCTGGTCGGCTTCGGGACATTCTTGGTCGGCA
AGCGATCTTCGCGTAATGGACGCAACCCGCGCACGGGTGCCGCCATCACAATCGAAGGCGGTAAGGTTGCGAAATTCAAA
CCTGGCAAAGCGCTGAAAGATGCGCTAAACTAA

Upstream 100 bases:

>100_bases
CGCTGGCTGCGACATGTGGCACACAGTTCTTCGCCTTGACACGTCGATTGACGGCCACTTTAATGAATCCACACTAGCGG
GTGTTCGGGGAGGTGGCACG

Downstream 100 bases:

>100_bases
CGGGCTTTGGTGCTCGTGGGTGACAACGCGGGCGCCGGACAGTCCGGCTCACGCCGGCAGTACGAACGGGTGCTTAGCTC
AGTTGGTAGAGCAGCGCCCT

Product: histone family protein DNA-binding protein

Products: NA

Alternate protein names: HU-1; NS1 [H]

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKTDLIDHVATQTDMSKAAAGRAIDALIDGIKDALCEGGSVTLVGFGTFLVGKRSSRNGRNPRTGAAITIEGGKVAKFK
PGKALKDALN

Sequences:

>Translated_90_residues
MNKTDLIDHVATQTDMSKAAAGRAIDALIDGIKDALCEGGSVTLVGFGTFLVGKRSSRNGRNPRTGAAITIEGGKVAKFK
PGKALKDALN
>Mature_90_residues
MNKTDLIDHVATQTDMSKAAAGRAIDALIDGIKDALCEGGSVTLVGFGTFLVGKRSSRNGRNPRTGAAITIEGGKVAKFK
PGKALKDALN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=58.8888888888889, Blast_Score=107, Evalue=1e-25,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=49.438202247191, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=36.2637362637363, Blast_Score=72, Evalue=5e-15,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9300; Mature: 9300

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTDLIDHVATQTDMSKAAAGRAIDALIDGIKDALCEGGSVTLVGFGTFLVGKRSSRNG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECEEEECCCCCCCC
RNPRTGAAITIEGGKVAKFKPGKALKDALN
CCCCCCEEEEECCCEEEEECCCHHHHHHCC
>Mature Secondary Structure
MNKTDLIDHVATQTDMSKAAAGRAIDALIDGIKDALCEGGSVTLVGFGTFLVGKRSSRNG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECEEEECCCCCCCC
RNPRTGAAITIEGGKVAKFKPGKALKDALN
CCCCCCEEEEECCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]