BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is benD [H]

Identifier: 187928454

GI number: 187928454

Start: 1465172

End: 1465954

Strand: Direct

Name: benD [H]

Synonym: Rpic_1367

Alternate gene names: 187928454

Gene position: 1465172-1465954 (Clockwise)

Preceding gene: 187928453

Following gene: 187928455

Centisome position: 37.16

GC content: 66.92

Gene sequence:

>783_bases
ATGAGCACGCAACGATTTTCAGGCAAGGCCGTCGTGGTGACGGGCGCAGCGCAGGGCATCGGCCGTGGCGTGGCAGTGGC
TGCGGCAGAAGAGGGGGCGCACGTGCTGCTGGTGGACCGCGCCGAACTCGTCCACGAAGTGCAGGCCGAGATTGCGGCAG
CGGGCGGCCACGCCCACGCCACCACGGCTGATCTCGAAACCTACGCCGGTGCCCAGCATGCCGTGCAGACTGCGCTTGGC
GCGTTCGGCCGCATCGACGTGCTCATCAACAACGTCGGCGGCACCATCTGGGCCAAGCCGTATCAGCACTATGAAGAGGC
GCAGATCGAGGCGGAGATCCGCCGTTCACTGTTTCCGACGCTGTGGTGCTGCCGCGCGGTGCTGCCGCACATGGTCGAGC
GCAAGCAGGGCGTGATCGTGAACGTGTCGTCCATCGCTACACGCGGCATCTACCGGATTCCGTACTCGGCGGCCAAGGGC
GGCGTCAATGCGCTCACCGCGAGTCTGGCGTTGGAACATGCGGACGACAACATTCGCGTCAACGCCGTCGCCACGGGCGG
CACGGAGGCACCGCCGCGCAAGATCCCGCGCAACGCGGCGCCCATGTCGGAGCAGGAAACCGTCTGGTACCAGGGCATCG
TCGACCAGACCCTCGCCAGCAGCCTGATGCACCGCTACGGCACGATCGACGAGCAGGTGCGGGCGATCCTGTTCCTCGCG
TCAGATGAGGCCTCGTACATCACCGGGACGGTACTGCCCGTCGGCGGCGGAGACCTGGGTTGA

Upstream 100 bases:

>100_bases
TAGACGCCGTGCGCAAGTACTTTGACGACCAAGGCGTGAAACCCAAGAGCTTCCACTACGAGAAGTTCACGCCCAATGCA
GTACCGGAGGCGAAGGCGGC

Downstream 100 bases:

>100_bases
AGGTTTGAAGCGCCAACAGAGCGCGCCATCAGTCGAAGAAGCCAACCCAGCATAAGAAAGGCGCTCACGCCTTCAAAAAA
AACCCCAAAGGAGACCACCC

Product: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

Products: NA

Alternate protein names: 2-hydro-1,2-dihydroxybenzoate dehydrogenase; DHB dehydrogenase; Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase; Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSTQRFSGKAVVVTGAAQGIGRGVAVAAAEEGAHVLLVDRAELVHEVQAEIAAAGGHAHATTADLETYAGAQHAVQTALG
AFGRIDVLINNVGGTIWAKPYQHYEEAQIEAEIRRSLFPTLWCCRAVLPHMVERKQGVIVNVSSIATRGIYRIPYSAAKG
GVNALTASLALEHADDNIRVNAVATGGTEAPPRKIPRNAAPMSEQETVWYQGIVDQTLASSLMHRYGTIDEQVRAILFLA
SDEASYITGTVLPVGGGDLG

Sequences:

>Translated_260_residues
MSTQRFSGKAVVVTGAAQGIGRGVAVAAAEEGAHVLLVDRAELVHEVQAEIAAAGGHAHATTADLETYAGAQHAVQTALG
AFGRIDVLINNVGGTIWAKPYQHYEEAQIEAEIRRSLFPTLWCCRAVLPHMVERKQGVIVNVSSIATRGIYRIPYSAAKG
GVNALTASLALEHADDNIRVNAVATGGTEAPPRKIPRNAAPMSEQETVWYQGIVDQTLASSLMHRYGTIDEQVRAILFLA
SDEASYITGTVLPVGGGDLG
>Mature_259_residues
STQRFSGKAVVVTGAAQGIGRGVAVAAAEEGAHVLLVDRAELVHEVQAEIAAAGGHAHATTADLETYAGAQHAVQTALGA
FGRIDVLINNVGGTIWAKPYQHYEEAQIEAEIRRSLFPTLWCCRAVLPHMVERKQGVIVNVSSIATRGIYRIPYSAAKGG
VNALTASLALEHADDNIRVNAVATGGTEAPPRKIPRNAAPMSEQETVWYQGIVDQTLASSLMHRYGTIDEQVRAILFLAS
DEASYITGTVLPVGGGDLG

Specific function: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=29.3436293436293, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4504505, Length=197, Percent_Identity=36.0406091370558, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI32483357, Length=258, Percent_Identity=28.2945736434109, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=28.90625, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI126723750, Length=264, Percent_Identity=27.6515151515151, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI66933014, Length=269, Percent_Identity=29.7397769516729, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=28.1746031746032, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI15277342, Length=267, Percent_Identity=29.2134831460674, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI210032110, Length=156, Percent_Identity=29.4871794871795, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI5031737, Length=270, Percent_Identity=28.1481481481481, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=32.8185328185328, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790717, Length=261, Percent_Identity=32.183908045977, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787905, Length=262, Percent_Identity=30.1526717557252, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789208, Length=260, Percent_Identity=28.4615384615385, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI2367175, Length=260, Percent_Identity=31.1538461538462, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=31.496062992126, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI87082160, Length=261, Percent_Identity=25.2873563218391, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=26.3779527559055, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=27.7992277992278, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=27.843137254902, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=32.5581395348837, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=276, Percent_Identity=32.2463768115942, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=32.295719844358, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=32.1969696969697, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17565030, Length=271, Percent_Identity=32.1033210332103, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=31.6981132075472, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=30.4182509505703, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=195, Percent_Identity=32.8205128205128, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=27.7992277992278, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=275, Percent_Identity=28, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=190, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=194, Percent_Identity=33.5051546391753, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17544670, Length=197, Percent_Identity=30.9644670050761, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=202, Percent_Identity=30.1980198019802, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=194, Percent_Identity=32.9896907216495, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=273, Percent_Identity=30.03663003663, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=29.8892988929889, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=199, Percent_Identity=31.6582914572864, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=199, Percent_Identity=32.1608040201005, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=29.0566037735849, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=209, Percent_Identity=31.1004784688995, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=26.7924528301887, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=262, Percent_Identity=28.6259541984733, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=168, Percent_Identity=30.3571428571429, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=28.3464566929134, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25152911, Length=198, Percent_Identity=34.8484848484849, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=171, Percent_Identity=29.8245614035088, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=195, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=168, Percent_Identity=29.7619047619048, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=193, Percent_Identity=33.6787564766839, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=26.8199233716475, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323882, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=35.408560311284, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571526, Length=262, Percent_Identity=35.4961832061069, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357041, Length=262, Percent_Identity=34.7328244274809, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=33.4600760456274, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=32.3076923076923, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355319, Length=271, Percent_Identity=26.9372693726937, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24639444, Length=271, Percent_Identity=29.1512915129151, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643142, Length=258, Percent_Identity=28.2945736434109, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644337, Length=263, Percent_Identity=32.319391634981, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357673, Length=196, Percent_Identity=25.5102040816327, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640492, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24649181, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580925, Length=190, Percent_Identity=32.1052631578947, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.25 [H]

Molecular weight: Translated: 27461; Mature: 27330

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQRFSGKAVVVTGAAQGIGRGVAVAAAEEGAHVLLVDRAELVHEVQAEIAAAGGHAHA
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCC
TTADLETYAGAQHAVQTALGAFGRIDVLINNVGGTIWAKPYQHYEEAQIEAEIRRSLFPT
CHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHH
LWCCRAVLPHMVERKQGVIVNVSSIATRGIYRIPYSAAKGGVNALTASLALEHADDNIRV
HHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEECCCHHHCCCHHHHHHHHHHEECCCCEEE
NAVATGGTEAPPRKIPRNAAPMSEQETVWYQGIVDQTLASSLMHRYGTIDEQVRAILFLA
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEE
SDEASYITGTVLPVGGGDLG
CCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
STQRFSGKAVVVTGAAQGIGRGVAVAAAEEGAHVLLVDRAELVHEVQAEIAAAGGHAHA
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCC
TTADLETYAGAQHAVQTALGAFGRIDVLINNVGGTIWAKPYQHYEEAQIEAEIRRSLFPT
CHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHH
LWCCRAVLPHMVERKQGVIVNVSSIATRGIYRIPYSAAKGGVNALTASLALEHADDNIRV
HHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEECCCHHHCCCHHHHHHHHHHEECCCCEEE
NAVATGGTEAPPRKIPRNAAPMSEQETVWYQGIVDQTLASSLMHRYGTIDEQVRAILFLA
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEEE
SDEASYITGTVLPVGGGDLG
CCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120 [H]