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The map label for this gene is gcvA [H]

Identifier: 187928415

GI number: 187928415

Start: 1420400

End: 1421344

Strand: Reverse

Name: gcvA [H]

Synonym: Rpic_1327

Alternate gene names: 187928415

Gene position: 1421344-1420400 (Counterclockwise)

Preceding gene: 187928424

Following gene: 187928414

Centisome position: 36.05

GC content: 68.57

Gene sequence:

>945_bases
ATGTTGACTCCCCTGGTTCGGTTGGCCTCCCTGGACTTGCTGCGCGGCTTCGTGGCGGTGGGCCGGCGCATGAGCATCAC
GCTCGCGGCCGATGATCTGTGTCTGACGCAATCCGCCGTAAGCCGCCAGGTGTCGGCGCTCGAAGAGGCGCTGGGCGTGA
AACTGCTGGTGCGCGGGCACCGCTCGATCGCGTTCACGCCCGAGGGAGAACGCCTGTTCCGCAGCGCTGACAGCGCCGTG
CAGCAGTTGCAGGACGCCATCGGGGCCATCCGCGTGGCGGCCGAACGCCGGCCAGTCACGATCACGGCCAGCATCGGCGT
CACCGGCTTGTGGCTGCTGCCGCGCCTGGCGCGCTTCCAGCTGCAGTACCCCGACATCGACGTGCGCGTGGCCACCAACA
ACCGCCTCGTCGACCTGAAGAACGAGGGCATCGAGCTTGCCATCCGGTATTGCCCACCGAGCGATGCACCGCCCGGTGCA
ATCCGGCTGTTTGGGGAAACCGTTGCGCCGGTCGCGCATCCGTCGCTGGGGCTGCGCTCGCTGGATTCCGCCGAGGCGCT
TGCCGGCCTGACGCTGCTCGACTTTGACGACCGCGACCTGCGCCGCCCCTGGCTCCAATGGACCGACTGGCTGGCCGCCG
CCGGATGGAGCCACGCCAAGCCGCGCACCGTGCTGCGCTTCAACCAGCACGACCAGATCATCCATGCGGCGGTGGCCGGC
CAGGGCATCGCGCTGGGCCGTCTGGAGTTGATCGCGCCGCTGCTGGCCGACGGCCGCTTGGTCACCGTACCGACCATCGC
CCCCGGCGTGCGCAATACGCATGCGTACTGGTTGATCCAATCCGAGCCGAACCCGCGCCGCGACGTGCGCGATGTGGCTG
CCTGGATCTGCTCGGAAGCTGCAACTCAGGCTACGCATGCGTCCGGCGCATCCGAACTTGCATGA

Upstream 100 bases:

>100_bases
GAATGCGCGTTGGGCTGGAATGTCGCCTGATGGCTTATTAAACTTCGCCCTACCCGGCTTCTCAATTGATCTTTGAGAAC
GCCTTGCATGAGGAAAACGC

Downstream 100 bases:

>100_bases
AAGGCGTTTGAGCCCACAGGGCGGTCCACGTGTAATGCCGCCAACGACACCCCGTTGGAGGCCGCATGGACACCCTCGCC
CGACCGCAGACAGCCCCCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLTPLVRLASLDLLRGFVAVGRRMSITLAADDLCLTQSAVSRQVSALEEALGVKLLVRGHRSIAFTPEGERLFRSADSAV
QQLQDAIGAIRVAAERRPVTITASIGVTGLWLLPRLARFQLQYPDIDVRVATNNRLVDLKNEGIELAIRYCPPSDAPPGA
IRLFGETVAPVAHPSLGLRSLDSAEALAGLTLLDFDDRDLRRPWLQWTDWLAAAGWSHAKPRTVLRFNQHDQIIHAAVAG
QGIALGRLELIAPLLADGRLVTVPTIAPGVRNTHAYWLIQSEPNPRRDVRDVAAWICSEAATQATHASGASELA

Sequences:

>Translated_314_residues
MLTPLVRLASLDLLRGFVAVGRRMSITLAADDLCLTQSAVSRQVSALEEALGVKLLVRGHRSIAFTPEGERLFRSADSAV
QQLQDAIGAIRVAAERRPVTITASIGVTGLWLLPRLARFQLQYPDIDVRVATNNRLVDLKNEGIELAIRYCPPSDAPPGA
IRLFGETVAPVAHPSLGLRSLDSAEALAGLTLLDFDDRDLRRPWLQWTDWLAAAGWSHAKPRTVLRFNQHDQIIHAAVAG
QGIALGRLELIAPLLADGRLVTVPTIAPGVRNTHAYWLIQSEPNPRRDVRDVAAWICSEAATQATHASGASELA
>Mature_314_residues
MLTPLVRLASLDLLRGFVAVGRRMSITLAADDLCLTQSAVSRQVSALEEALGVKLLVRGHRSIAFTPEGERLFRSADSAV
QQLQDAIGAIRVAAERRPVTITASIGVTGLWLLPRLARFQLQYPDIDVRVATNNRLVDLKNEGIELAIRYCPPSDAPPGA
IRLFGETVAPVAHPSLGLRSLDSAEALAGLTLLDFDDRDLRRPWLQWTDWLAAAGWSHAKPRTVLRFNQHDQIIHAAVAG
QGIALGRLELIAPLLADGRLVTVPTIAPGVRNTHAYWLIQSEPNPRRDVRDVAAWICSEAATQATHASGASELA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=31.4569536423841, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=34.251968503937, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=31.5589353612167, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=30.2681992337165, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=160, Percent_Identity=32.5, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=136, Percent_Identity=31.6176470588235, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787589, Length=273, Percent_Identity=27.1062271062271, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788887, Length=191, Percent_Identity=29.3193717277487, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=31.9277108433735, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34103; Mature: 34103

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLVRLASLDLLRGFVAVGRRMSITLAADDLCLTQSAVSRQVSALEEALGVKLLVRGH
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEEECC
RSIAFTPEGERLFRSADSAVQQLQDAIGAIRVAAERRPVTITASIGVTGLWLLPRLARFQ
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHE
LQYPDIDVRVATNNRLVDLKNEGIELAIRYCPPSDAPPGAIRLFGETVAPVAHPSLGLRS
EECCCEEEEEECCCEEEEECCCCEEEEEEECCCCCCCCCEEEECCCHHCCCCCCCCCCCC
LDSAEALAGLTLLDFDDRDLRRPWLQWTDWLAAAGWSHAKPRTVLRFNQHDQIIHAAVAG
CCCHHHHHCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHCC
QGIALGRLELIAPLLADGRLVTVPTIAPGVRNTHAYWLIQSEPNPRRDVRDVAAWICSEA
CCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
ATQATHASGASELA
HHHHHHCCCHHCCC
>Mature Secondary Structure
MLTPLVRLASLDLLRGFVAVGRRMSITLAADDLCLTQSAVSRQVSALEEALGVKLLVRGH
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEEECC
RSIAFTPEGERLFRSADSAVQQLQDAIGAIRVAAERRPVTITASIGVTGLWLLPRLARFQ
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHE
LQYPDIDVRVATNNRLVDLKNEGIELAIRYCPPSDAPPGAIRLFGETVAPVAHPSLGLRS
EECCCEEEEEECCCEEEEECCCCEEEEEEECCCCCCCCCEEEECCCHHCCCCCCCCCCCC
LDSAEALAGLTLLDFDDRDLRRPWLQWTDWLAAAGWSHAKPRTVLRFNQHDQIIHAAVAG
CCCHHHHHCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHCC
QGIALGRLELIAPLLADGRLVTVPTIAPGVRNTHAYWLIQSEPNPRRDVRDVAAWICSEA
CCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
ATQATHASGASELA
HHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]