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The map label for this gene is ywbI [H]

Identifier: 187928424

GI number: 187928424

Start: 1430130

End: 1431023

Strand: Reverse

Name: ywbI [H]

Synonym: Rpic_1336

Alternate gene names: 187928424

Gene position: 1431023-1430130 (Counterclockwise)

Preceding gene: 187928425

Following gene: 187928415

Centisome position: 36.3

GC content: 64.77

Gene sequence:

>894_bases
ATGCCCATCGATATCCGAGCCCTTCGTTACTTTGTCGAAACCGCGCGGCTGCAGAGCTTCACGCAGGCGGCGAGCTCGCT
CTCGGTGACACAGTCCACCATCAGCAAGATGGTGCGTCAGCTCGAAAACGAGGTCGGCCAGCCGCTGCTGATCCGCGAAG
GCCGGCAGGTGCGGCTCACTGACGTGGGCCGGGTCGTGTACGCACGCGGGCAGGAAACGCTGGGCGTGGTGCACAGGCTG
ACGCTTGAAGTCGCTGATCTGTCCTCGCTCGGACGGGGCGAACTGACGGTCGGCATCCCACCGATGGTGAATCTGTTCTT
TTCGCCCGTCGTGAGCGCGTTTCGTCAGCGTTATCCGAATCTGGTACTGAACTTGGCAGAGTACGGCGGCAAAACGGTGG
AACAGCAGGTGGCCAGCGGCGAACTGGAAGTGGGCGCCACCGTTCTGCCCGGCGATAGTGGGCTCGAACTCGCCACGCGA
CAGTTTGCAAAGTATCCGATCTGGGTGGTGGGACCGCGCAAAGCGAAATGGGCCAAAGGCAGGACCGTCACCCTCACGGC
ACTGCGCGACGAACCGCTGCTATTGCTGGCCGAGGATTTCTCCCTGACGCGCAAGCTGCGACAGGCCTTTCTCGAAGCCC
GTATCGAGCCGCACATCGCTGCGCGGAGCGGGCATTGGGAATTCCTCGCCTCGATGGCCGCTGCGGGACTGGGCACGACT
TTTTTGCCGCAGCCGCTGGTGGCCAACCTCGCGAACCGTGACAAGCTGGCCGTGGCACGCCTGACCGCCCCCGACATGGA
TTGGGCGCTCGCGCACCTGTGGTCGCCCGGCCGCTACCTGTCGCACGCCGCTCGCGCGTGGCTCGCGACGTGCCAGGAAG
TGCTCGGCGCCTGA

Upstream 100 bases:

>100_bases
ACACAGGCCCGCACGATGTTGAGGCCATCGCGTCGTGGAGTGGGAAAACTTCATTACCATGGCGAATTCCATTCGCGTGC
TTCATGGCTAAGGTCCGTCC

Downstream 100 bases:

>100_bases
GCAGCGGCTGCGACGCGCGTCAAGCGCTGAGGGCGGGTTCCGGGAGCAGACTTTCACCTGTGAGCCCGGCTGAGGCCATC
GAGCCCTGGTCGCGAAACCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPIDIRALRYFVETARLQSFTQAASSLSVTQSTISKMVRQLENEVGQPLLIREGRQVRLTDVGRVVYARGQETLGVVHRL
TLEVADLSSLGRGELTVGIPPMVNLFFSPVVSAFRQRYPNLVLNLAEYGGKTVEQQVASGELEVGATVLPGDSGLELATR
QFAKYPIWVVGPRKAKWAKGRTVTLTALRDEPLLLLAEDFSLTRKLRQAFLEARIEPHIAARSGHWEFLASMAAAGLGTT
FLPQPLVANLANRDKLAVARLTAPDMDWALAHLWSPGRYLSHAARAWLATCQEVLGA

Sequences:

>Translated_297_residues
MPIDIRALRYFVETARLQSFTQAASSLSVTQSTISKMVRQLENEVGQPLLIREGRQVRLTDVGRVVYARGQETLGVVHRL
TLEVADLSSLGRGELTVGIPPMVNLFFSPVVSAFRQRYPNLVLNLAEYGGKTVEQQVASGELEVGATVLPGDSGLELATR
QFAKYPIWVVGPRKAKWAKGRTVTLTALRDEPLLLLAEDFSLTRKLRQAFLEARIEPHIAARSGHWEFLASMAAAGLGTT
FLPQPLVANLANRDKLAVARLTAPDMDWALAHLWSPGRYLSHAARAWLATCQEVLGA
>Mature_296_residues
PIDIRALRYFVETARLQSFTQAASSLSVTQSTISKMVRQLENEVGQPLLIREGRQVRLTDVGRVVYARGQETLGVVHRLT
LEVADLSSLGRGELTVGIPPMVNLFFSPVVSAFRQRYPNLVLNLAEYGGKTVEQQVASGELEVGATVLPGDSGLELATRQ
FAKYPIWVVGPRKAKWAKGRTVTLTALRDEPLLLLAEDFSLTRKLRQAFLEARIEPHIAARSGHWEFLASMAAAGLGTTF
LPQPLVANLANRDKLAVARLTAPDMDWALAHLWSPGRYLSHAARAWLATCQEVLGA

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=30.3135888501742, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790399, Length=294, Percent_Identity=27.891156462585, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788297, Length=267, Percent_Identity=25.4681647940075, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=27.1812080536913, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=28.0276816608997, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=28, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=22.8, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=24.4897959183673, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=24.0157480314961, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=30.952380952381, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=140, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786401, Length=119, Percent_Identity=33.6134453781513, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32650; Mature: 32519

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDIRALRYFVETARLQSFTQAASSLSVTQSTISKMVRQLENEVGQPLLIREGRQVRLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
DVGRVVYARGQETLGVVHRLTLEVADLSSLGRGELTVGIPPMVNLFFSPVVSAFRQRYPN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCHH
LVLNLAEYGGKTVEQQVASGELEVGATVLPGDSGLELATRQFAKYPIWVVGPRKAKWAKG
HHHHHHHHCCHHHHHHHHCCCEEECCEECCCCCCHHHHHHHHHHCCEEEECCCCCCCCCC
RTVTLTALRDEPLLLLAEDFSLTRKLRQAFLEARIEPHIAARSGHWEFLASMAAAGLGTT
CEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCC
FLPQPLVANLANRDKLAVARLTAPDMDWALAHLWSPGRYLSHAARAWLATCQEVLGA
CCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIDIRALRYFVETARLQSFTQAASSLSVTQSTISKMVRQLENEVGQPLLIREGRQVRLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
DVGRVVYARGQETLGVVHRLTLEVADLSSLGRGELTVGIPPMVNLFFSPVVSAFRQRYPN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCHH
LVLNLAEYGGKTVEQQVASGELEVGATVLPGDSGLELATRQFAKYPIWVVGPRKAKWAKG
HHHHHHHHCCHHHHHHHHCCCEEECCEECCCCCCHHHHHHHHHHCCEEEECCCCCCCCCC
RTVTLTALRDEPLLLLAEDFSLTRKLRQAFLEARIEPHIAARSGHWEFLASMAAAGLGTT
CEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCC
FLPQPLVANLANRDKLAVARLTAPDMDWALAHLWSPGRYLSHAARAWLATCQEVLGA
CCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]