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The map label for this gene is ligA

Identifier: 187928361

GI number: 187928361

Start: 1363511

End: 1365964

Strand: Direct

Name: ligA

Synonym: Rpic_1272

Alternate gene names: 187928361

Gene position: 1363511-1365964 (Clockwise)

Preceding gene: 187928360

Following gene: 187928362

Centisome position: 34.58

GC content: 65.2

Gene sequence:

>2454_bases
ATGAGCAAGACTGAAGAACCCGCGTCCGGACCGCCCTCAAAAGACCGGCCCGAGGCGCGGGCAGCCTGGCTGCGCGCCGA
GCTGAACCGCTACGCCCACGAGTACTACGTGCTCGACCAGCCCAGTGTGCCCGACGTTGAATACGACCGGCTCTATCGCG
AGCTGGAAGCGCTGGAAACCGAACACCCCGAGCTGAAGACACCCGATTCGCCCACGCTGCGGGTCGGTGGCGCTGTCCTG
CCCGAATTTGCGGCGGTCAGGCATGTCATCCCGATGCTGTCGATCCGCACCGAGACCGACACCACCGCAAACGGCGCGCG
CGCGTTCGACGCCAGCGTAAGGCGCGAGCTGGGCCTTGATGACACCGATCCGCCCGTGGAATACGCCGCCGAGCTGAAGT
TCGATGGCCTGGCGATCAACCTGCGTTACGAGCGTGGTTATCTCGTGCAGGCGGCCACGCGCGGCGACGGCACCACCGGC
GAAGACGTCACGCAGAACATCCGCACGATCCGCCAGATTCCGCTGGGGCTGTCGCCAGTTGATGGCCACGTGCCCGACGT
GCTGGAAGTGCGCGGCGAGGTCTACATGCGCCGCGACGACTTCGAGAAGCTCAACGCCCGCCAGCGAGAGCGCGGTGACA
AGACCTTCGTCAACCCGCGCAATACCGCAGCCGGTGCCGTCCGCCAGCTCGATCCTAAGATGGCTGCCGAGCGCCCGCTG
TCGTTCTTCGCCTATGGGCTGGGCGAAGTGGCCGGCTGGAAGGGCATGCCCGGCACGCATTCCAGCATGCTCGACGTGCT
GCAGGCGTTCGGTTTCCCGGTCAGTAATGAACGCGCGGCTGTGGCGGGCGGTGAAGGCCTGGTCAGATTCCATGAGGCCA
TCCGGGCCAAGCGCGACAGCTTGCCGTTCGACATCGACGGCGTCGTCTACAAGGTCAACTCGCTCGCGTTGCAGCGTGAA
CTCGGCTTTCGCTCACGTGAGCCGCGCTGGGCGGTTGCGCACAAATATCCGGCGCAGGAAGCGCTGACCACGGTCGAATC
TATTGACGTGCAAGTCGGCCGAACGGGCGCCATCACGCCGGTGGCGCGGCTGGTGCCGGTGTTTGTTGGCGGGGTAACGG
TCACCAATGCGACGCTGCACAACGAAGACGAAGTGCGCCGCAAAGATGTACGCGTGGGCGACACCGTGATCGTGCGGCGC
GCGGGCGACGTGATTCCCGAGGTGGTGAGTGTGGTGCTTGATCGCCGCCCACTGGATGACGTCCCGGGCAGCGACTTGTT
CAATCCGCAGCAGCAGCCCAAGTACCCGCCATTCGAACTGCCGAAGACGTGCCCGGTTTGCGGCTCGCACGTCGTGCGCG
AAGAGGGTGAGGCGGTGGCCCGTTGCTCCGGCGGCCTGTTCTGCTCGGCGCAGCGCAAGGAGGCGATCCGCCACTTTGCC
GGTCGCCGCATGATGGACATCGAAGGCCTGGGCGAGCGTTACATCGACAACCTGGTCGAGTTGGACTACGTGCACGGCAT
TGCCGACCTGTACAAGCTCACGCTCGAAGATTTTCTGGAGATGAAGCGCCGTGCGGACGAACGTGATGGCGTGACGCCCG
AGACGGTTTCCGCAGGCAAGATCGCCACCAAGTGGGCCGAGAATCTGCTGGAGGGCATCCAGGCCAGCAAGACGCCGCCG
CTGGCGCGCTTCCTGTTTGCGCTGGGCATTCGCCACGTGGGCGAGTCGACCGCCAAGACGCTTGCCGACTGGCTCGGCAG
CCTGGCCATGATCCGGCGTGCGCCTGCGCCGCTGCTGCTGGCGCTGCCCGATGTGGGTGCCACGGTGGCTGAAGCCATCG
CCGATTTCTTTGCCGAGCCGAAGAACCAGCAGGCGCTCGATGCGCTGCTCGAAGCCGGTGTTGCACCGCAAGGCGAGCAT
GCGCCCAACGCGAAGCTGCGCGAGAAGCTGGAGCCGGCCGAACTGTACGCCACGCTGGGCGTGCCCAAGCTGACCGCCAC
GCGCGCAAAGCAATTGGCCGTGGCAGCGCCAACGCTTGCCCAGTTGGCCAGCGTTGAGCCCGATCAGTTGGCTGGCCTGC
CTGCGGATGTGACTGCATCGCTGCTTGAATGGCTCGCCTCGCACGACCATCGCGCGCAACTGGCGAAGCTCGACGCGCTG
CGCGGCGAGTTGCTCGCTGCCATGCCTGCTGAGGCCGCCGAAGAAGGCGTGCTGGACGGCAAGACGGTCGTTTTGACCGG
CACATTGCCGAACCTCACGCGTGACGAAGCCAAGGCCATGCTGGAAGCGGCAGGCGCCAAGGTATCGGGTTCGGTGTCGA
AAAAGACCGACTACGTTGTCGCCGGAGAAGATGCCGGCAGCAAGCTCGCCAAAGCCGAAGAATTGAACGTGAAGGTTCTG
GACGAAGCGGGTATGCTCGCGCTGTTAAAAAAACCGGCGGGAGACCAGGCATGA

Upstream 100 bases:

>100_bases
CAGAAGCATCTGCGGGTGCTGTATGACAAGCTCGAATCGCGCGGCATCCCGGCCGGTTCCAGCACCGCGGTGCGCCTGTT
CAGCCACTGACGGACGCACG

Downstream 100 bases:

>100_bases
TCCGAAACATCCTCAAGATGGGCGACAGCCGGCTGCTGCGCGTTGCCAAGCCGGTCGAGCGTTTCCACACGCCCGAACTG
ACTGCCCTGATCGAAGACAT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 817; Mature: 816

Protein sequence:

>817_residues
MSKTEEPASGPPSKDRPEARAAWLRAELNRYAHEYYVLDQPSVPDVEYDRLYRELEALETEHPELKTPDSPTLRVGGAVL
PEFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLDDTDPPVEYAAELKFDGLAINLRYERGYLVQAATRGDGTTG
EDVTQNIRTIRQIPLGLSPVDGHVPDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPL
SFFAYGLGEVAGWKGMPGTHSSMLDVLQAFGFPVSNERAAVAGGEGLVRFHEAIRAKRDSLPFDIDGVVYKVNSLALQRE
LGFRSREPRWAVAHKYPAQEALTTVESIDVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRR
AGDVIPEVVSVVLDRRPLDDVPGSDLFNPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFA
GRRMMDIEGLGERYIDNLVELDYVHGIADLYKLTLEDFLEMKRRADERDGVTPETVSAGKIATKWAENLLEGIQASKTPP
LARFLFALGIRHVGESTAKTLADWLGSLAMIRRAPAPLLLALPDVGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEH
APNAKLREKLEPAELYATLGVPKLTATRAKQLAVAAPTLAQLASVEPDQLAGLPADVTASLLEWLASHDHRAQLAKLDAL
RGELLAAMPAEAAEEGVLDGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDYVVAGEDAGSKLAKAEELNVKVL
DEAGMLALLKKPAGDQA

Sequences:

>Translated_817_residues
MSKTEEPASGPPSKDRPEARAAWLRAELNRYAHEYYVLDQPSVPDVEYDRLYRELEALETEHPELKTPDSPTLRVGGAVL
PEFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLDDTDPPVEYAAELKFDGLAINLRYERGYLVQAATRGDGTTG
EDVTQNIRTIRQIPLGLSPVDGHVPDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPL
SFFAYGLGEVAGWKGMPGTHSSMLDVLQAFGFPVSNERAAVAGGEGLVRFHEAIRAKRDSLPFDIDGVVYKVNSLALQRE
LGFRSREPRWAVAHKYPAQEALTTVESIDVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRR
AGDVIPEVVSVVLDRRPLDDVPGSDLFNPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFA
GRRMMDIEGLGERYIDNLVELDYVHGIADLYKLTLEDFLEMKRRADERDGVTPETVSAGKIATKWAENLLEGIQASKTPP
LARFLFALGIRHVGESTAKTLADWLGSLAMIRRAPAPLLLALPDVGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEH
APNAKLREKLEPAELYATLGVPKLTATRAKQLAVAAPTLAQLASVEPDQLAGLPADVTASLLEWLASHDHRAQLAKLDAL
RGELLAAMPAEAAEEGVLDGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDYVVAGEDAGSKLAKAEELNVKVL
DEAGMLALLKKPAGDQA
>Mature_816_residues
SKTEEPASGPPSKDRPEARAAWLRAELNRYAHEYYVLDQPSVPDVEYDRLYRELEALETEHPELKTPDSPTLRVGGAVLP
EFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLDDTDPPVEYAAELKFDGLAINLRYERGYLVQAATRGDGTTGE
DVTQNIRTIRQIPLGLSPVDGHVPDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLS
FFAYGLGEVAGWKGMPGTHSSMLDVLQAFGFPVSNERAAVAGGEGLVRFHEAIRAKRDSLPFDIDGVVYKVNSLALQREL
GFRSREPRWAVAHKYPAQEALTTVESIDVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRA
GDVIPEVVSVVLDRRPLDDVPGSDLFNPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAG
RRMMDIEGLGERYIDNLVELDYVHGIADLYKLTLEDFLEMKRRADERDGVTPETVSAGKIATKWAENLLEGIQASKTPPL
ARFLFALGIRHVGESTAKTLADWLGSLAMIRRAPAPLLLALPDVGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEHA
PNAKLREKLEPAELYATLGVPKLTATRAKQLAVAAPTLAQLASVEPDQLAGLPADVTASLLEWLASHDHRAQLAKLDALR
GELLAAMPAEAAEEGVLDGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDYVVAGEDAGSKLAKAEELNVKVLD
EAGMLALLKKPAGDQA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=84, Percent_Identity=46.4285714285714, Blast_Score=76, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=617, Percent_Identity=48.7844408427877, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI87082305, Length=414, Percent_Identity=25.1207729468599, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RALPJ (B2UB00)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898848.1
- ProteinModelPortal:   B2UB00
- GeneID:   6286326
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK337493
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 88735; Mature: 88604

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 133-133 BINDING 131-131 BINDING 154-154 BINDING 193-193 BINDING 311-311 BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTEEPASGPPSKDRPEARAAWLRAELNRYAHEYYVLDQPSVPDVEYDRLYRELEALET
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHC
EHPELKTPDSPTLRVGGAVLPEFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLD
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHCCCC
DTDPPVEYAAELKFDGLAINLRYERGYLVQAATRGDGTTGEDVTQNIRTIRQIPLGLSPV
CCCCCHHHHHHEEECCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
DGHVPDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPL
CCCCCHHHHHCCHHEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCH
SFFAYGLGEVAGWKGMPGTHSSMLDVLQAFGFPVSNERAAVAGGEGLVRFHEAIRAKRDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCC
LPFDIDGVVYKVNSLALQRELGFRSREPRWAVAHKYPAQEALTTVESIDVQVGRTGAITP
CCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHEEECCCCCCHH
VARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVSVVLDRRPLDD
HHHHHHHHHCCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
VPGSDLFNPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCEEECHHHHHHHHHHH
GRRMMDIEGLGERYIDNLVELDYVHGIADLYKLTLEDFLEMKRRADERDGVTPETVSAGK
CCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCH
IATKWAENLLEGIQASKTPPLARFLFALGIRHVGESTAKTLADWLGSLAMIRRAPAPLLL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEE
ALPDVGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEHAPNAKLREKLEPAELYATLG
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCC
VPKLTATRAKQLAVAAPTLAQLASVEPDQLAGLPADVTASLLEWLASHDHRAQLAKLDAL
CCHHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RGELLAAMPAEAAEEGVLDGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDYVV
HHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
AGEDAGSKLAKAEELNVKVLDEAGMLALLKKPAGDQA
ECCCCCCHHHHHHHCCEEEECCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SKTEEPASGPPSKDRPEARAAWLRAELNRYAHEYYVLDQPSVPDVEYDRLYRELEALET
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHC
EHPELKTPDSPTLRVGGAVLPEFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLD
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHCCCC
DTDPPVEYAAELKFDGLAINLRYERGYLVQAATRGDGTTGEDVTQNIRTIRQIPLGLSPV
CCCCCHHHHHHEEECCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
DGHVPDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPL
CCCCCHHHHHCCHHEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCH
SFFAYGLGEVAGWKGMPGTHSSMLDVLQAFGFPVSNERAAVAGGEGLVRFHEAIRAKRDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHCC
LPFDIDGVVYKVNSLALQRELGFRSREPRWAVAHKYPAQEALTTVESIDVQVGRTGAITP
CCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHEEECCCCCCHH
VARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVSVVLDRRPLDD
HHHHHHHHHCCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
VPGSDLFNPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCEEECHHHHHHHHHHH
GRRMMDIEGLGERYIDNLVELDYVHGIADLYKLTLEDFLEMKRRADERDGVTPETVSAGK
CCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCH
IATKWAENLLEGIQASKTPPLARFLFALGIRHVGESTAKTLADWLGSLAMIRRAPAPLLL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEE
ALPDVGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEHAPNAKLREKLEPAELYATLG
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCC
VPKLTATRAKQLAVAAPTLAQLASVEPDQLAGLPADVTASLLEWLASHDHRAQLAKLDAL
CCHHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RGELLAAMPAEAAEEGVLDGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDYVV
HHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE
AGEDAGSKLAKAEELNVKVLDEAGMLALLKKPAGDQA
ECCCCCCHHHHHHHCCEEEECCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA