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The map label for this gene is def2 [H]

Identifier: 187928362

GI number: 187928362

Start: 1365961

End: 1366494

Strand: Direct

Name: def2 [H]

Synonym: Rpic_1273

Alternate gene names: 187928362

Gene position: 1365961-1366494 (Clockwise)

Preceding gene: 187928361

Following gene: 187928363

Centisome position: 34.65

GC content: 63.48

Gene sequence:

>534_bases
ATGATCCGAAACATCCTCAAGATGGGCGACAGCCGGCTGCTGCGCGTTGCCAAGCCGGTCGAGCGTTTCCACACGCCCGA
ACTGACTGCCCTGATCGAAGACATGTTCGACACCATGGATGCGGCGCGCGGTGCCGGTCTGGCAGCCCCGCAGATCGGCG
TGGATCTACAGGTCGTCATCTTCGGGTTCGATCGTAACGACCGGTATCCGGATGCACCGGCCGTGCCGAAGACGGTGTTG
ATCAACCCCACCATCGAGCCGCTGTCGGACGAGATGGAAGAGGGCTGGGAAGGATGCCTCTCGGTACCCGGCCTGCGCGG
TGTGGTGCCGCGCTACACGCGCCTGCGCTATACCGGCTTCGACCAGCACGGCCACGCCATCGACCGTATTGCCGAGGGCT
TCCACGCGCGCGTCGTCCAGCACGAATGCGATCACCTGCAGGGCGTGCTGTATCCCATGCGCGTGAAGGACTTCACCCGC
TTCGGCTTTACCGAAGTGCTGTTCCCGGAATTGCCCGCCAATCACGACGACTGA

Upstream 100 bases:

>100_bases
ATGCCGGCAGCAAGCTCGCCAAAGCCGAAGAATTGAACGTGAAGGTTCTGGACGAAGCGGGTATGCTCGCGCTGTTAAAA
AAACCGGCGGGAGACCAGGC

Downstream 100 bases:

>100_bases
TCGAGACTGCGGCAGCGTCGCATCTGCGGGGGCGGGTGGACGCGTGTTTCATCCACCACGGAGACGATATGAGGCGAGAT
GGTTTGAGGGGCTGGCGCCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIRNILKMGDSRLLRVAKPVERFHTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDEMEEGWEGCLSVPGLRGVVPRYTRLRYTGFDQHGHAIDRIAEGFHARVVQHECDHLQGVLYPMRVKDFTR
FGFTEVLFPELPANHDD

Sequences:

>Translated_177_residues
MIRNILKMGDSRLLRVAKPVERFHTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDEMEEGWEGCLSVPGLRGVVPRYTRLRYTGFDQHGHAIDRIAEGFHARVVQHECDHLQGVLYPMRVKDFTR
FGFTEVLFPELPANHDD
>Mature_177_residues
MIRNILKMGDSRLLRVAKPVERFHTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDEMEEGWEGCLSVPGLRGVVPRYTRLRYTGFDQHGHAIDRIAEGFHARVVQHECDHLQGVLYPMRVKDFTR
FGFTEVLFPELPANHDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=176, Percent_Identity=33.5227272727273, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=37.5838926174497, Blast_Score=94, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645728, Length=182, Percent_Identity=30.2197802197802, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19983; Mature: 19983

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRNILKMGDSRLLRVAKPVERFHTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVI
CCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FGFDRNDRYPDAPAVPKTVLINPTIEPLSDEMEEGWEGCLSVPGLRGVVPRYTRLRYTGF
EEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCC
DQHGHAIDRIAEGFHARVVQHECDHLQGVLYPMRVKDFTRFGFTEVLFPELPANHDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIRNILKMGDSRLLRVAKPVERFHTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVI
CCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FGFDRNDRYPDAPAVPKTVLINPTIEPLSDEMEEGWEGCLSVPGLRGVVPRYTRLRYTGF
EEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCC
DQHGHAIDRIAEGFHARVVQHECDHLQGVLYPMRVKDFTRFGFTEVLFPELPANHDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]