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The map label for this gene is pit [H]

Identifier: 187928277

GI number: 187928277

Start: 1274793

End: 1275803

Strand: Direct

Name: pit [H]

Synonym: Rpic_1188

Alternate gene names: 187928277

Gene position: 1274793-1275803 (Clockwise)

Preceding gene: 187928276

Following gene: 187928290

Centisome position: 32.33

GC content: 62.61

Gene sequence:

>1011_bases
ATGCATACGCTTCAGATCAGTCTCTGGGTGGTCGTTCTGCTGGTGGCGCTGGCCATTGTGTTCGACTTCATGAATGGCTT
TCACGACGCGGCCAACTCCATTGCGACGGTGGTGTCGACCGGCGTGCTCAAGCCGCAGCAGGCAGTGGCCATGGCCGCTG
CCTGCAACGTGATCGCCATTTTCATCTTCCACCTCAAGGTGGCAACCACGGTGGGACGGGACACGATTGATCCGTCCATC
GTCGATCATTACGTCATCTTCGGGGCGTTGGTCGGCGCCATCGCGTGGAACATCATCACGTGGTACTACGGCATTCCGTC
CAGCTCGTCGCACGCGCTCATCGGTGGCTTGGTAGGCGCGGCTGTGGCCAAGTCCGGCACGGGCTCGCTGGTGGCCAGTG
GCTTGCTCAAGACCGTGGCGTTCATCCTGATTTCGCCGTTGCTGGGTTTTATCCTCGGCTCGCTCTTGATGGTGGCGGTG
TCCTGGCTGTTCTTCCGAACGCCGCCATCCAAGGTGGACCGATGGTTCCGGCGCTTGCAACTGCTCTCCGCTTCGCTGTA
CAGCCTGGGCCATGGCGGCAACGATGCGCAGAAGACCATCGGCATCATCTGGATGCTGCTGATTGCGGGCGGCTACGCGT
CGGCCACGGCAACCGAACCGCCGATCTGGGTCATCGTCTGCTGCTATGTGGCGATCGGCTTGGGTACGCTGCTGGGTGGC
TGGCGGATCGTGCGCACGATGGGTCAGAAGATCACCAAGCTCAAGCCGGTCGGCGGTTTCTGCGCGGAAACCGGTGGCGC
CATGACGCTGTTCTTCGCTTCGGCGTTGGGCGTGCCAGTGTCGACGACGCACACCATCACAGGCGCCATTGTCGGTGTGG
GCGCGGCGCAGAAAGCTTCTGCCGTGCGCTGGGGGGTGGCAGGCAACATCGTCTGGGCGTGGGTGTTGACGATTCCCGCG
TCGGCGTTCATGGCGGCCATCGCCTGGTGGATCGGTCGACACGTGCTGTAA

Upstream 100 bases:

>100_bases
CGATCTACGAACAACTGGAGAGCATCACCGACCGTTGCGAGGATGTCGCCAACATCATCGAAGGCATCGTGCTCGAAAAC
GCCTGACGAGACGACGCCTC

Downstream 100 bases:

>100_bases
GCAAAGCCGGCCTCGGATTCACTTCGCAAAAACGAAACGCCCACCAAACCGGTGGGCGTTTTGCATTGGGGCGAGCGATG
CGCTACTGCGTGGCAAAGCG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHTLQISLWVVVLLVALAIVFDFMNGFHDAANSIATVVSTGVLKPQQAVAMAAACNVIAIFIFHLKVATTVGRDTIDPSI
VDHYVIFGALVGAIAWNIITWYYGIPSSSSHALIGGLVGAAVAKSGTGSLVASGLLKTVAFILISPLLGFILGSLLMVAV
SWLFFRTPPSKVDRWFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIAGGYASATATEPPIWVIVCCYVAIGLGTLLGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFFASALGVPVSTTHTITGAIVGVGAAQKASAVRWGVAGNIVWAWVLTIPA
SAFMAAIAWWIGRHVL

Sequences:

>Translated_336_residues
MHTLQISLWVVVLLVALAIVFDFMNGFHDAANSIATVVSTGVLKPQQAVAMAAACNVIAIFIFHLKVATTVGRDTIDPSI
VDHYVIFGALVGAIAWNIITWYYGIPSSSSHALIGGLVGAAVAKSGTGSLVASGLLKTVAFILISPLLGFILGSLLMVAV
SWLFFRTPPSKVDRWFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIAGGYASATATEPPIWVIVCCYVAIGLGTLLGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFFASALGVPVSTTHTITGAIVGVGAAQKASAVRWGVAGNIVWAWVLTIPA
SAFMAAIAWWIGRHVL
>Mature_336_residues
MHTLQISLWVVVLLVALAIVFDFMNGFHDAANSIATVVSTGVLKPQQAVAMAAACNVIAIFIFHLKVATTVGRDTIDPSI
VDHYVIFGALVGAIAWNIITWYYGIPSSSSHALIGGLVGAAVAKSGTGSLVASGLLKTVAFILISPLLGFILGSLLMVAV
SWLFFRTPPSKVDRWFRRLQLLSASLYSLGHGGNDAQKTIGIIWMLLIAGGYASATATEPPIWVIVCCYVAIGLGTLLGG
WRIVRTMGQKITKLKPVGGFCAETGGAMTLFFASALGVPVSTTHTITGAIVGVGAAQKASAVRWGVAGNIVWAWVLTIPA
SAFMAAIAWWIGRHVL

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=175, Percent_Identity=35.4285714285714, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=37.6623376623377, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.3364055299539, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789360, Length=221, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=33.1325301204819, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=32.0987654320988, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=31.4814814814815, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566716, Length=175, Percent_Identity=31.4285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536725, Length=186, Percent_Identity=29.5698924731183, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=169, Percent_Identity=31.9526627218935, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=165, Percent_Identity=30.9090909090909, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=31.0160427807487, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35317; Mature: 35317

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]