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The map label for this gene is cobB [H]

Identifier: 187928167

GI number: 187928167

Start: 1142982

End: 1143731

Strand: Direct

Name: cobB [H]

Synonym: Rpic_1074

Alternate gene names: 187928167

Gene position: 1142982-1143731 (Clockwise)

Preceding gene: 187928166

Following gene: 187928169

Centisome position: 28.99

GC content: 66.53

Gene sequence:

>750_bases
ATGAGCATCGAACCCTCGGTCGCATCCGATGCGGCCGCATTGGCCCAGGCCCGCGCCTGGGTCGAGGCCGCCCAGCACGT
CATGGTGCTCACGGGCGCGGGCGTCTCGGCCGAATCCGGTGTCCCTACGTTTCGTGATGCCCTGACTGGCTTGTGGTCGC
GCTTCAACCCGGAAGACATGGCGACCGAGGCCGCGTACCGCAAGCACCCCCGCATGGTGTGGGACTGGTATCAGGAGCGC
CGCGAGCGTGTGCTGCAGGTGCAGCCGAATCCGGCCCATCTTGCAATTGCCGCGCTAGCCAAGCGCAAGGCGGTGACGCT
CGTCACGCAGAACGTCGACGGTTTGCATCAGCGCGCCGGCAGCGAAGGGGTGATCGAGCTGCACGGCAACCTGTTTGCCA
ACAAGTGGTTGGGTGGCTGCGGCAAATGCGATATCGCCACCGCCGAGCCGGGCAGGCCGCCGCGTTGTGCGACGTGTGGC
GCGATGCTGCGCCCGGGCGTGGTGTGGTTTGGCGAGCAACTGCCGCTGGTCGCGAACTACCGTGCTGAAGAGGCGGCCAA
TACGTGCGACGTTTGTCTGGTAGTTGGCACATCCGGCATGGTATATCCGGCCGCTGGCTTGCCGGGGCTGGCGAAGGACC
ATGGCGCCAAGGTGATCGTCGTGAATCCGGAGCCAAGCGCCCTGGATGCAACGGCGGACCTCGTGATCCACCAGCCGGCG
GGCGTGTGCCTGCCCGCCATGCTGGGATAA

Upstream 100 bases:

>100_bases
CGATGGCCGGCAGCGTTGAAAGCCTGAACGTGTCGGTAGCCAGCGGGGTTTGCCTGTTTGAGGCTGTGCGCCAACGCGCA
GCGGCACCTGCCAGGAAGTA

Downstream 100 bases:

>100_bases
GACCCGGCTTTCTTCTTACGCCGCTGCGCGCAGCGGCTCAATCAACGCTTCCAACTTGATCGCGTCCGCTGCAAACAGGC
GGATGCCTTCGGCCAGCTTT

Product: Silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSIEPSVASDAAALAQARAWVEAAQHVMVLTGAGVSAESGVPTFRDALTGLWSRFNPEDMATEAAYRKHPRMVWDWYQER
RERVLQVQPNPAHLAIAALAKRKAVTLVTQNVDGLHQRAGSEGVIELHGNLFANKWLGGCGKCDIATAEPGRPPRCATCG
AMLRPGVVWFGEQLPLVANYRAEEAANTCDVCLVVGTSGMVYPAAGLPGLAKDHGAKVIVVNPEPSALDATADLVIHQPA
GVCLPAMLG

Sequences:

>Translated_249_residues
MSIEPSVASDAAALAQARAWVEAAQHVMVLTGAGVSAESGVPTFRDALTGLWSRFNPEDMATEAAYRKHPRMVWDWYQER
RERVLQVQPNPAHLAIAALAKRKAVTLVTQNVDGLHQRAGSEGVIELHGNLFANKWLGGCGKCDIATAEPGRPPRCATCG
AMLRPGVVWFGEQLPLVANYRAEEAANTCDVCLVVGTSGMVYPAAGLPGLAKDHGAKVIVVNPEPSALDATADLVIHQPA
GVCLPAMLG
>Mature_248_residues
SIEPSVASDAAALAQARAWVEAAQHVMVLTGAGVSAESGVPTFRDALTGLWSRFNPEDMATEAAYRKHPRMVWDWYQERR
ERVLQVQPNPAHLAIAALAKRKAVTLVTQNVDGLHQRAGSEGVIELHGNLFANKWLGGCGKCDIATAEPGRPPRCATCGA
MLRPGVVWFGEQLPLVANYRAEEAANTCDVCLVVGTSGMVYPAAGLPGLAKDHGAKVIVVNPEPSALDATADLVIHQPAG
VCLPAMLG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=259, Percent_Identity=38.2239382239382, Blast_Score=167, Evalue=6e-42,
Organism=Homo sapiens, GI6912664, Length=276, Percent_Identity=36.5942028985507, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI13787215, Length=252, Percent_Identity=38.0952380952381, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI7657575, Length=235, Percent_Identity=31.063829787234, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI6912662, Length=278, Percent_Identity=28.0575539568345, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300797597, Length=214, Percent_Identity=32.2429906542056, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI300797577, Length=233, Percent_Identity=30.0429184549356, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI13775602, Length=202, Percent_Identity=28.7128712871287, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI13775600, Length=202, Percent_Identity=28.7128712871287, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI6912660, Length=211, Percent_Identity=31.2796208530806, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI300797705, Length=193, Percent_Identity=27.979274611399, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI63054862, Length=190, Percent_Identity=30.5263157894737, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI308199517, Length=227, Percent_Identity=41.4096916299559, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17567771, Length=276, Percent_Identity=26.0869565217391, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71990482, Length=276, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17541892, Length=228, Percent_Identity=28.5087719298246, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17505510, Length=246, Percent_Identity=28.8617886178862, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571445, Length=284, Percent_Identity=28.5211267605634, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648389, Length=225, Percent_Identity=29.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137536, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26378; Mature: 26246

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEPSVASDAAALAQARAWVEAAQHVMVLTGAGVSAESGVPTFRDALTGLWSRFNPEDM
CCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ATEAAYRKHPRMVWDWYQERRERVLQVQPNPAHLAIAALAKRKAVTLVTQNVDGLHQRAG
HHHHHHHHCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHEEEEEECCCCHHHHHCC
SEGVIELHGNLFANKWLGGCGKCDIATAEPGRPPRCATCGAMLRPGVVWFGEQLPLVANY
CCCEEEECCCEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECC
RAEEAANTCDVCLVVGTSGMVYPAAGLPGLAKDHGAKVIVVNPEPSALDATADLVIHQPA
CHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCC
GVCLPAMLG
CCCHHHHCC
>Mature Secondary Structure 
SIEPSVASDAAALAQARAWVEAAQHVMVLTGAGVSAESGVPTFRDALTGLWSRFNPEDM
CCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ATEAAYRKHPRMVWDWYQERRERVLQVQPNPAHLAIAALAKRKAVTLVTQNVDGLHQRAG
HHHHHHHHCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHEEEEEECCCCHHHHHCC
SEGVIELHGNLFANKWLGGCGKCDIATAEPGRPPRCATCGAMLRPGVVWFGEQLPLVANY
CCCEEEECCCEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEECC
RAEEAANTCDVCLVVGTSGMVYPAAGLPGLAKDHGAKVIVVNPEPSALDATADLVIHQPA
CHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCC
GVCLPAMLG
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]