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The map label for this gene is rlmB [H]

Identifier: 187928166

GI number: 187928166

Start: 1142230

End: 1142982

Strand: Direct

Name: rlmB [H]

Synonym: Rpic_1073

Alternate gene names: 187928166

Gene position: 1142230-1142982 (Clockwise)

Preceding gene: 187928165

Following gene: 187928167

Centisome position: 28.97

GC content: 65.6

Gene sequence:

>753_bases
ATGTCTAAATCCAAGCTCCTCATCGGCTTTCATGCCGTCACCGCCCGACTGCGCCACGGCGCCAAGTCGATCGACGAAAT
CTACTTCGAGGCCAACCGCCGCGACCGCCGCATGCAGGATTTCCTGAAAGCGGCGGAAGCAGCGGGCGTGCGCCTCATTC
CCGCTGACGCCGACCGTCTGCGCGGCATTGCCGGTACCGACCGCCACCAGGGCGTGGTTGCCAAGGCAGAGTCGCTGTCG
TTGGCGCTCAATCTTGATGAACTGCTCGACGGCATCGAAGGCACGCCGCTGCTGCTGGTGCTCGACGGTGTGACGGACCC
GCACAACCTCGGCGCTTGCCTGCGCGTGGCCGATGCTGCCGGCGCGCATGCCGTGATCGCGCCGAAGGACCGCAGCGTTG
GGATCAACACGACGGTCGCCAAGGTCGCCAGCGGCGCGGCTGAAACCGTCCCGTATATCACCGTGACCAACCTGGCGCGC
ACGCTGCGTGAATTGCAGGAGCGCGGCGTGTGGGTGATCGGCACGGCTGACGAGGCCGAACAGGATCTCTACCAGGCAGA
CTTCAAGGGCCCCACCGCCATCGTGATGGGCGCCGAAGGCGAAGGCATGCGCCGCCTCACGCGTGAAACGTGCGACGCGC
TGGTCAGCATTCCGATGGCCGGCAGCGTTGAAAGCCTGAACGTGTCGGTAGCCAGCGGGGTTTGCCTGTTTGAGGCTGTG
CGCCAACGCGCAGCGGCACCTGCCAGGAAGTAA

Upstream 100 bases:

>100_bases
CAAGACCGCTGCGCCGCGCAAGGTGGCAGGCAAGGCCCCGCGCAAGACCAAGGGGCGTTAAAGCGGCCGGGGAGCGAGGG
CGGGCGGGTACAATCGCGCC

Downstream 100 bases:

>100_bases
TGAGCATCGAACCCTCGGTCGCATCCGATGCGGCCGCATTGGCCCAGGCCCGCGCCTGGGTCGAGGCCGCCCAGCACGTC
ATGGTGCTCACGGGCGCGGG

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSKSKLLIGFHAVTARLRHGAKSIDEIYFEANRRDRRMQDFLKAAEAAGVRLIPADADRLRGIAGTDRHQGVVAKAESLS
LALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLAR
TLRELQERGVWVIGTADEAEQDLYQADFKGPTAIVMGAEGEGMRRLTRETCDALVSIPMAGSVESLNVSVASGVCLFEAV
RQRAAAPARK

Sequences:

>Translated_250_residues
MSKSKLLIGFHAVTARLRHGAKSIDEIYFEANRRDRRMQDFLKAAEAAGVRLIPADADRLRGIAGTDRHQGVVAKAESLS
LALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLAR
TLRELQERGVWVIGTADEAEQDLYQADFKGPTAIVMGAEGEGMRRLTRETCDALVSIPMAGSVESLNVSVASGVCLFEAV
RQRAAAPARK
>Mature_249_residues
SKSKLLIGFHAVTARLRHGAKSIDEIYFEANRRDRRMQDFLKAAEAAGVRLIPADADRLRGIAGTDRHQGVVAKAESLSL
ALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADAAGAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLART
LRELQERGVWVIGTADEAEQDLYQADFKGPTAIVMGAEGEGMRRLTRETCDALVSIPMAGSVESLNVSVASGVCLFEAVR
QRAAAPARK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=265, Percent_Identity=27.1698113207547, Blast_Score=87, Evalue=9e-18,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=54.7325102880658, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788935, Length=190, Percent_Identity=28.9473684210526, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26564; Mature: 26432

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSKLLIGFHAVTARLRHGAKSIDEIYFEANRRDRRMQDFLKAAEAAGVRLIPADADRL
CCCCCEEEHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCHHHH
RGIAGTDRHQGVVAKAESLSLALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADAA
CCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLARTLRELQERGVWVIGTADEAE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
QDLYQADFKGPTAIVMGAEGEGMRRLTRETCDALVSIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHHHHHHHHHH
RQRAAAPARK
HHHHCCCCCC
>Mature Secondary Structure 
SKSKLLIGFHAVTARLRHGAKSIDEIYFEANRRDRRMQDFLKAAEAAGVRLIPADADRL
CCCCEEEHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCHHHH
RGIAGTDRHQGVVAKAESLSLALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADAA
CCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GAHAVIAPKDRSVGINTTVAKVASGAAETVPYITVTNLARTLRELQERGVWVIGTADEAE
CCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
QDLYQADFKGPTAIVMGAEGEGMRRLTRETCDALVSIPMAGSVESLNVSVASGVCLFEAV
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHHHHHHHHHH
RQRAAAPARK
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]