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The map label for this gene is pcm [H]

Identifier: 187928142

GI number: 187928142

Start: 1115012

End: 1115956

Strand: Direct

Name: pcm [H]

Synonym: Rpic_1049

Alternate gene names: 187928142

Gene position: 1115012-1115956 (Clockwise)

Preceding gene: 187928141

Following gene: 187928143

Centisome position: 28.28

GC content: 67.41

Gene sequence:

>945_bases
ATGTCTGAACGCCCGCGCGATCGGCGCTTCCCGTTGACGCTCGACGCGGTGGTCGAGCGTAAGCCCGTGGAGCGCCAGCG
TGAGACGCGTATCTCGTCTGGGGTGAGCGCGGTGTCACGTCCCACGCCGGTGCGTGCGGCATCCGTCGAACGCGCGTCAC
CCGCATCCGCCCCCGCACCTGCGCCGCAACGCGTGGCGGGGGTCGACGCTGCGGTCGGCGCCGTCGCTGCGAGCGCCGTG
CAGGCACGCACGGCGGCAGCGCATGCGGCGGCGGGCGGCATGGCGTCGGACCGCGCGCGTCAGGCATTGGTTGGCCGGGT
GCAAGCAGGTGGCGTGAGTGACAAGCGCGTGCTGGCAGCAATCGGCACTGTGCCACGCCATCTGTTCGTCGATGCCGGCC
TGGCGTCGCAGGCGTATGAGGATTCCGCATTACCGATCGGCCATCAGCAAACGATCTCGAAGCCGTCAGTGGTCGGACGG
ATGATCGAGTTGTTGTTGAGTGGACGGTCGGGCCGCACCGGCATGCCGCTGGCACGTGTGCTGGAAATTGGCACCGGCTG
CGGCTACCAGGCAGCCGTTCTGGCGCAACTCGCCGACGAGGTGTACTCCATCGAGCGCGTTCGCCCGCTGCACGAAAAAG
CCAAAGCGAACTTGCGGCCACTCCGCGTGCCAAACATCCGTCTGCACTATGGCGACGGCATGCTGGGGCTGCCGAAGGCC
GCGCCGTTCGACGCCATCATCCTGGCGGCGGCGGGGCTGGAGGTGCCGCAGGCGTTGCTCGACCAGCTGACCGTCGGCGG
CCGGCTGATCGCCCCCGTTGCCACCGACCGCAACAGCGGTCCGGCGCAGCAGCTCGTTCTCATTGAGCGTCGCAGCCGCT
TCGAGTTTCACAGTACCGCGCTTGAAGGCGTTTTCTTTGTCCCGTTAAAATCAGGAACTGTTTAG

Upstream 100 bases:

>100_bases
CGGCTTCGTCTCGCTGACACCGCTGCAGCTCGATCTGACCGATACGGCCCAACTGCGCACCGTGCGCCGCTGGCAGACGC
CATGATCGCCACGGTGGCTC

Downstream 100 bases:

>100_bases
CATGTTCATTTCCCGGAGCTCCATGAACCGTCCAAGCCCCGCACGCGCAGGCCGGCTCGCCGTGGCGATGGTGTCGGCCG
CACTACTGGCGGCTTGCGCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSERPRDRRFPLTLDAVVERKPVERQRETRISSGVSAVSRPTPVRAASVERASPASAPAPAPQRVAGVDAAVGAVAASAV
QARTAAAHAAAGGMASDRARQALVGRVQAGGVSDKRVLAAIGTVPRHLFVDAGLASQAYEDSALPIGHQQTISKPSVVGR
MIELLLSGRSGRTGMPLARVLEIGTGCGYQAAVLAQLADEVYSIERVRPLHEKAKANLRPLRVPNIRLHYGDGMLGLPKA
APFDAIILAAAGLEVPQALLDQLTVGGRLIAPVATDRNSGPAQQLVLIERRSRFEFHSTALEGVFFVPLKSGTV

Sequences:

>Translated_314_residues
MSERPRDRRFPLTLDAVVERKPVERQRETRISSGVSAVSRPTPVRAASVERASPASAPAPAPQRVAGVDAAVGAVAASAV
QARTAAAHAAAGGMASDRARQALVGRVQAGGVSDKRVLAAIGTVPRHLFVDAGLASQAYEDSALPIGHQQTISKPSVVGR
MIELLLSGRSGRTGMPLARVLEIGTGCGYQAAVLAQLADEVYSIERVRPLHEKAKANLRPLRVPNIRLHYGDGMLGLPKA
APFDAIILAAAGLEVPQALLDQLTVGGRLIAPVATDRNSGPAQQLVLIERRSRFEFHSTALEGVFFVPLKSGTV
>Mature_313_residues
SERPRDRRFPLTLDAVVERKPVERQRETRISSGVSAVSRPTPVRAASVERASPASAPAPAPQRVAGVDAAVGAVAASAVQ
ARTAAAHAAAGGMASDRARQALVGRVQAGGVSDKRVLAAIGTVPRHLFVDAGLASQAYEDSALPIGHQQTISKPSVVGRM
IELLLSGRSGRTGMPLARVLEIGTGCGYQAAVLAQLADEVYSIERVRPLHEKAKANLRPLRVPNIRLHYGDGMLGLPKAA
PFDAIILAAAGLEVPQALLDQLTVGGRLIAPVATDRNSGPAQQLVLIERRSRFEFHSTALEGVFFVPLKSGTV

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=183, Percent_Identity=34.9726775956284, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789100, Length=221, Percent_Identity=43.8914027149321, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=182, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193207222, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17981723, Length=184, Percent_Identity=33.695652173913, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 32988; Mature: 32857

Theoretical pI: Translated: 11.17; Mature: 11.17

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERPRDRRFPLTLDAVVERKPVERQRETRISSGVSAVSRPTPVRAASVERASPASAPAP
CCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCCHHCCCCCCCCCCCCC
APQRVAGVDAAVGAVAASAVQARTAAAHAAAGGMASDRARQALVGRVQAGGVSDKRVLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IGTVPRHLFVDAGLASQAYEDSALPIGHQQTISKPSVVGRMIELLLSGRSGRTGMPLARV
HHHCCHHHHEECCCHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
LEIGTGCGYQAAVLAQLADEVYSIERVRPLHEKAKANLRPLRVPNIRLHYGDGMLGLPKA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCCCCCCC
APFDAIILAAAGLEVPQALLDQLTVGGRLIAPVATDRNSGPAQQLVLIERRSRFEFHSTA
CCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHH
LEGVFFVPLKSGTV
HCCEEEEEECCCCC
>Mature Secondary Structure 
SERPRDRRFPLTLDAVVERKPVERQRETRISSGVSAVSRPTPVRAASVERASPASAPAP
CCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCCHHCCCCCCCCCCCCC
APQRVAGVDAAVGAVAASAVQARTAAAHAAAGGMASDRARQALVGRVQAGGVSDKRVLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IGTVPRHLFVDAGLASQAYEDSALPIGHQQTISKPSVVGRMIELLLSGRSGRTGMPLARV
HHHCCHHHHEECCCHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
LEIGTGCGYQAAVLAQLADEVYSIERVRPLHEKAKANLRPLRVPNIRLHYGDGMLGLPKA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCCCCCCC
APFDAIILAAAGLEVPQALLDQLTVGGRLIAPVATDRNSGPAQQLVLIERRSRFEFHSTA
CCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHH
LEGVFFVPLKSGTV
HCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]