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The map label for this gene is pmbA [H]

Identifier: 187927911

GI number: 187927911

Start: 859213

End: 860586

Strand: Direct

Name: pmbA [H]

Synonym: Rpic_0816

Alternate gene names: 187927911

Gene position: 859213-860586 (Clockwise)

Preceding gene: 187927908

Following gene: 187927914

Centisome position: 21.79

GC content: 63.32

Gene sequence:

>1374_bases
ATGTCTCAAACGCTCGATTCCAAGACCGCCCCTGTGTTTGCCTACCGCCGCGAACAGCTCGAGGAGATGGCCTCCGATGT
GCTGCGCATTGCACGCGAACTCGGCGCCTCTGATGCCGCAACGGAAATCTCCGAAGGCCAGGGCCTGTCGGTCACGGTGC
GCAAGGGCGAGATCGAGACCATCGAGCAGAACCGCGACAAGATTGTTGGCGTGACCGTCATGCTGGGCAAGAAGCGCGGC
AACGCCAGCACGTCGGACTTTTCGCCGGCAGCACTGCGGTCCACCGTCGAGGCCGCCTACAACATCGCGCGCTTCACAGC
CGACGATGATTGCGCCGGCTTGGCAGAAGAAGAACTGCTGGAGAAGCACCCGCGAGACTTGCAGCTCTATCACCCATGGA
TGATCGACGCCGAAGCCGCCGTGGAGATCGCGCGCGCCGCTGAAGCCGCTGCCTTTGCCGTGAGCCCAAAGATCCGCAAC
AGCGATGGTTCCAGCATCTCTGCCCAGCATTCGCACTTCGTGTTGGCCACGTCGCGCGGTTTCATGGCGGGCTACCCGTA
TTCGCGTCACTTCATTTCCGCCGCGCCGATCGCCGGTTCGGGCGCGCATATGCAGCGCGATGATTGGTACTCGTCCAAGC
GCTCGCCGGACGATCTGGCCGCGCCGGAAGCGATCGGCAAGTACGCGGCGGAACGCGCGCTGGCGCGTTTGTCCGCGCGC
CGGTTGACGACCCGCAAGTGCCCGGTGTTGTTTGAGGCGCCGCTGGCTGCGGGTTTGCTGGGCGCGTTCGTGCAGGCCGT
GTCGGGAGGCGCCTTGTACCGCAAGTCGACGTTCCTGCTCGATACGCTGGGCAAGGAAGTGTTCGCGCCGCATGTGCAGA
TCAAGGAAGACCCGCATGTTCTCAACGGGATGGGCAGCGCGCCCTTCGACGAGGAAGGCGTACGCACGCAGCGCCGCGAT
GTGGTGAAGAACGGCGTGGTGGAGGGCTACTTCCTGTCGACGTATTCCGCGCGCAAGCTCGGCATGCAGACTACTGGCAA
CGCGGGCGGCTCGCATAACCTGACGCTGTTCTCCAACAAAACGACCGCCGAAGATGACTTTGCCGGCATGCTGCGCCGCA
TGGGCACGGGTCTGCTCGTGACCGAACTGATGGGCCAGGGCGTGAACTATGTGACGGGCGACTATTCGCGCGGCGCGTCG
GGCTATTGGGTGGAGAACGGCGTGATTCAGTATCCGGTGGAAGAGATCACGATTGCCGGCAACCTGGGCGAGATGTTCCG
CCAGATTGTCGCCATCGGCGCGGATGCGCTAGTGCGCGGCACGAAGGAAACCGGCTCGATCCTGATCGAACAGATGACAA
TTGCCGGCGAATAA

Upstream 100 bases:

>100_bases
TCGGGATGCTCGGCTCATGGCGCGCATTGTATCTTGCTATGATGGGCGCTTCCGCCCGCTTTTGGCAAAATCGCGGCACC
CGTTTCTCCCTTTATTGCCT

Downstream 100 bases:

>100_bases
ATTGCCACAGCGGAAATAAAAAAACGCGCTCGAAACTGAGCGCGTTTTTTATTGCCTGCAGCGGTTATTGCTCGTTCGAT
GGCGGACGTTCATAGGTAAC

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSQTLDSKTAPVFAYRREQLEEMASDVLRIARELGASDAATEISEGQGLSVTVRKGEIETIEQNRDKIVGVTVMLGKKRG
NASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLEKHPRDLQLYHPWMIDAEAAVEIARAAEAAAFAVSPKIRN
SDGSSISAQHSHFVLATSRGFMAGYPYSRHFISAAPIAGSGAHMQRDDWYSSKRSPDDLAAPEAIGKYAAERALARLSAR
RLTTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDTLGKEVFAPHVQIKEDPHVLNGMGSAPFDEEGVRTQRRD
VVKNGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSNKTTAEDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGAS
GYWVENGVIQYPVEEITIAGNLGEMFRQIVAIGADALVRGTKETGSILIEQMTIAGE

Sequences:

>Translated_457_residues
MSQTLDSKTAPVFAYRREQLEEMASDVLRIARELGASDAATEISEGQGLSVTVRKGEIETIEQNRDKIVGVTVMLGKKRG
NASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLEKHPRDLQLYHPWMIDAEAAVEIARAAEAAAFAVSPKIRN
SDGSSISAQHSHFVLATSRGFMAGYPYSRHFISAAPIAGSGAHMQRDDWYSSKRSPDDLAAPEAIGKYAAERALARLSAR
RLTTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDTLGKEVFAPHVQIKEDPHVLNGMGSAPFDEEGVRTQRRD
VVKNGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSNKTTAEDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGAS
GYWVENGVIQYPVEEITIAGNLGEMFRQIVAIGADALVRGTKETGSILIEQMTIAGE
>Mature_456_residues
SQTLDSKTAPVFAYRREQLEEMASDVLRIARELGASDAATEISEGQGLSVTVRKGEIETIEQNRDKIVGVTVMLGKKRGN
ASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLEKHPRDLQLYHPWMIDAEAAVEIARAAEAAAFAVSPKIRNS
DGSSISAQHSHFVLATSRGFMAGYPYSRHFISAAPIAGSGAHMQRDDWYSSKRSPDDLAAPEAIGKYAAERALARLSARR
LTTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDTLGKEVFAPHVQIKEDPHVLNGMGSAPFDEEGVRTQRRDV
VKNGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSNKTTAEDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASG
YWVENGVIQYPVEEITIAGNLGEMFRQIVAIGADALVRGTKETGSILIEQMTIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=443, Percent_Identity=47.4040632054176, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1789640, Length=397, Percent_Identity=23.6775818639798, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49257; Mature: 49126

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLDSKTAPVFAYRREQLEEMASDVLRIARELGASDAATEISEGQGLSVTVRKGEIET
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHH
IEQNRDKIVGVTVMLGKKRGNASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELL
HHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EKHPRDLQLYHPWMIDAEAAVEIARAAEAAAFAVSPKIRNSDGSSISAQHSHFVLATSRG
HHCCCCEEEECCEEECHHHHHHHHHHHHHHHEEECCCEECCCCCCEECCCCEEEEEECCC
FMAGYPYSRHFISAAPIAGSGAHMQRDDWYSSKRSPDDLAAPEAIGKYAAERALARLSAR
CEECCCCHHHHEEECCCCCCCCCCCCCHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
RLTTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDTLGKEVFAPHVQIKEDPHV
HHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCEECCCCHH
LNGMGSAPFDEEGVRTQRRDVVKNGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSNK
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECC
TTAEDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAG
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEEECHHHEEEEC
NLGEMFRQIVAIGADALVRGTKETGSILIEQMTIAGE
CHHHHHHHHHHHCCHHHHCCCCHHHHEEEEHHEECCC
>Mature Secondary Structure 
SQTLDSKTAPVFAYRREQLEEMASDVLRIARELGASDAATEISEGQGLSVTVRKGEIET
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHH
IEQNRDKIVGVTVMLGKKRGNASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELL
HHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EKHPRDLQLYHPWMIDAEAAVEIARAAEAAAFAVSPKIRNSDGSSISAQHSHFVLATSRG
HHCCCCEEEECCEEECHHHHHHHHHHHHHHHEEECCCEECCCCCCEECCCCEEEEEECCC
FMAGYPYSRHFISAAPIAGSGAHMQRDDWYSSKRSPDDLAAPEAIGKYAAERALARLSAR
CEECCCCHHHHEEECCCCCCCCCCCCCHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
RLTTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDTLGKEVFAPHVQIKEDPHV
HHHHCCCCEEEECHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCEECCCCHH
LNGMGSAPFDEEGVRTQRRDVVKNGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSNK
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECC
TTAEDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAG
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEEECHHHEEEEC
NLGEMFRQIVAIGADALVRGTKETGSILIEQMTIAGE
CHHHHHHHHHHHCCHHHHCCCCHHHHEEEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]