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The map label for this gene is gyrB [H]

Identifier: 187927115

GI number: 187927115

Start: 3438

End: 5966

Strand: Direct

Name: gyrB [H]

Synonym: Rpic_0003

Alternate gene names: 187927115

Gene position: 3438-5966 (Clockwise)

Preceding gene: 187927114

Following gene: 187927116

Centisome position: 0.09

GC content: 63.74

Gene sequence:

>2529_bases
ATGACCGAAGAGCAGAAACCGCAATCCACCCCCGCCGAGGCCAACAGCTATGACGCCGCCTCGATCCAGATCCTGGAAGG
CCTGGAGGCGGTACGCAAGCGGCCGGGCATGTACATCGGCGACACCTCGGATGGCACCGGCCTGCACCACCTCGTGTTCG
AGGTGCTGGACAATTCCATCGACGAAGCACTGGCCGGGCATTGCACCGAGATCCAGGTCACCATCCACACGGACAACTCC
ATCTCCGTGATCGACAACGGCCGTGGCATCCCGACCGGCATCAAGTTTGACGACAAACACGAGCCCAAGCGCAGCGCGAC
CGAGATCGCCATGACCGAGCTGCACGCCGGTGGCAAGTTCAACCAGAACAGCTACAAGGTGTCGGGCGGCCTGCACGGCG
TGGGCGTGTCGTGCGTGAACGGTCTGTCGAAGTGGATGAAGGTGACGGTGCGCCAGGGTGGCAAGGTGCATTACATCGAG
TTTGCACAGGGCGTTCCGCAAAACCGCCTGATCGAAACGGTGCAAGGGCCGGACGGTCAGATGGTGGAGGTCTCGCCGCT
GCGCGTGTTGGGCACCACCGACAAGCGCGGCACCGAAGTGCACTTCCTGGCCGACGAAGAGATCTTCACCAACGTCGAAT
ACCACTACGAGATCCTCTCCAAGCGCATTCGCGAGCTTTCGTTCCTGAACAACGGCGTGCACATCCGGCTGACCGATCAG
CGCACCGGCAAGGAAGAAGACTTCGCCTTCTCGGGCGGCGTGAAGGGCTTCGTTGAGTACATCAACAAGAACAAGACCGT
GCTGCACCCCACCATCTTCTTTGCCGCAGGCGAGAAAGACGGCGTGGCCGTGGAAGTGTCAATGCAGTGGAACGACGCCT
ACAACGAACAGGTGCTCTGCTTCACCAACAACATCCCGCAGCGTGACGGCGGCACCCACCTCACGGGCCTGCGCGCCGCC
ATGACGCGCGTCATCAACAAGTACATCGCCGACAACGAGATCGCCAAGAAAGCCAAGGTCGACACCTCGGGCGACGACAT
GCGCGAAGGCCTGACCTGCGTGCTGTCCGTCAAGGTGCCCGAGCCCAAGTTCAGCTCGCAGACCAAGGACAAGCTCGTCT
CGTCGGAAGTGCGCCTGCCGGTGGAAGAAGTCGTCGCCAAGGCGCTGAGCGACTTCCTGCTGGAAACGCCCGTCGACGCC
AAGATCATCTGCGGCAAGATCGTTGAAGCTGCCCGCGCGCGTGAAGCCGCCCGCAAGGCCCGCGAAATGACGCGCCGCAA
AGGCGTGCTCGACGGCATGGGCCTGCCCGGCAAGCTGGCCGACTGCCAGGAGAAAGACCCCGCGCTGTCTGAACTCTTCA
TCGTCGAGGGTGACTCCGCAGGCGGATCGGCCAAGCAGGGCCGCGACCGCAAGTTCCAGGCAATCCTGCCGCTCAAGGGC
AAGATCCTGAACGTCGAGCGCGCGCGCTTTGACAAGATGCTCTCCAGCCAGGAAGTGCTCACGCTCATCACCGCCATGGG
CACCGGCATTGGCAAAGACGACTACAACCTCGACAAGCTGCGCTACCACCGCATCATCATCATGACCGATGCTGACGTGG
ACGGCTCGCACATCCGCACGCTGCTGCTCACGTTCTTCTACCGCCAGATGCCCGAGATCATCGAGCGCGGCCACGTGTAC
ATCGCCCAGCCGCCGCTCTACAAGATCAAGCACGGCAAGGAAGAGCGCTACATCAAAGACGATGTCGAGATGGCCGCCTA
CCTCGTGCGCCAGGCGCTGGACACCGCCGTGCTCGTGCGCCCCGACGGCACCGAGATTGCCGGCGACGCCCTGGCCGAAC
TCGCACGCCAGTACCAGCTCAGCCGCGGCGTGATCGAGCGCCTGTCTCGCGTGATTGACGCCGACGCCCTGCGCGCCATT
GCCGAAGGCGTGTCGCTGGACTTGTCCAACGAAGCCGCAGCCGAAGCCAGCGCCCGCACGCTCAAGGCCCGCCTGCTGGA
AATGCAAGGCAACGCCAGCAGCGCCAACGGCGGCGCCACCGCAGACGCCTTCATGCAGTACGACGAGAAGACCGAGAAGT
ACCGCATCATGGTCGTCCGCCGCCAGCACGGCAACCAGCGCCTCAGCCACATCGACGCCGACTTTGTGGCCGGCGCCGAC
TACACCGCGCTCTCAAGCACCGCCGCCACCTTCCAGGGCCTGATTGGCGACGGCGCCAAGGTGCGCCGCGGCACCGGCGA
CAAGGTGCGCGAGCAGAACGTTGCCGACTTCCACGCCGCCATGACCTGGCTGCTGAGCGAGGCCGAGCGCGGCGTGAGCC
GCCAGCGCTACAAGGGCCTGGGCGAAATGAACCCCGAGCAGCTGTGGGAAACCACCATGGACGTCACCCAGCGCCGCCTG
CTGCGCGTGCAGATTGAAGACGCCATTGCCGCGGACCAGATCTTCACCACGCTGATGGGCGACGACGTGGAACCGCGCCG
GAACTTCATTGAGAGCAATGCGTTGGTGGCGCGGAATATTGATGTTTGA

Upstream 100 bases:

>100_bases
GGGCTGGATCGTGACGATCCGCCCCTTCGCCGTTTTTAAGCAACCCCGCTGTGTGCGTTGAGATAACCATTTCCGCGCCC
ATGGCAGCGAGCAGAACGAC

Downstream 100 bases:

>100_bases
GGGGTGTAGCCGCGCATAGATACAAAACGCCGGGGGTTACTTCCCGGCGTTTTGCTTTGCACAGCACCAACGAGGCGTTT
CCGGCTGGAGTTGAAGCGGG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MTEEQKPQSTPAEANSYDAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIQVTIHTDNS
ISVIDNGRGIPTGIKFDDKHEPKRSATEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNGLSKWMKVTVRQGGKVHYIE
FAQGVPQNRLIETVQGPDGQMVEVSPLRVLGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIRLTDQ
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTIFFAAGEKDGVAVEVSMQWNDAYNEQVLCFTNNIPQRDGGTHLTGLRAA
MTRVINKYIADNEIAKKAKVDTSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALSDFLLETPVDA
KIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVY
IAQPPLYKIKHGKEERYIKDDVEMAAYLVRQALDTAVLVRPDGTEIAGDALAELARQYQLSRGVIERLSRVIDADALRAI
AEGVSLDLSNEAAAEASARTLKARLLEMQGNASSANGGATADAFMQYDEKTEKYRIMVVRRQHGNQRLSHIDADFVAGAD
YTALSSTAATFQGLIGDGAKVRRGTGDKVREQNVADFHAAMTWLLSEAERGVSRQRYKGLGEMNPEQLWETTMDVTQRRL
LRVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV

Sequences:

>Translated_842_residues
MTEEQKPQSTPAEANSYDAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIQVTIHTDNS
ISVIDNGRGIPTGIKFDDKHEPKRSATEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNGLSKWMKVTVRQGGKVHYIE
FAQGVPQNRLIETVQGPDGQMVEVSPLRVLGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIRLTDQ
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTIFFAAGEKDGVAVEVSMQWNDAYNEQVLCFTNNIPQRDGGTHLTGLRAA
MTRVINKYIADNEIAKKAKVDTSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALSDFLLETPVDA
KIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVY
IAQPPLYKIKHGKEERYIKDDVEMAAYLVRQALDTAVLVRPDGTEIAGDALAELARQYQLSRGVIERLSRVIDADALRAI
AEGVSLDLSNEAAAEASARTLKARLLEMQGNASSANGGATADAFMQYDEKTEKYRIMVVRRQHGNQRLSHIDADFVAGAD
YTALSSTAATFQGLIGDGAKVRRGTGDKVREQNVADFHAAMTWLLSEAERGVSRQRYKGLGEMNPEQLWETTMDVTQRRL
LRVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV
>Mature_841_residues
TEEQKPQSTPAEANSYDAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCTEIQVTIHTDNSI
SVIDNGRGIPTGIKFDDKHEPKRSATEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNGLSKWMKVTVRQGGKVHYIEF
AQGVPQNRLIETVQGPDGQMVEVSPLRVLGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIRLTDQR
TGKEEDFAFSGGVKGFVEYINKNKTVLHPTIFFAAGEKDGVAVEVSMQWNDAYNEQVLCFTNNIPQRDGGTHLTGLRAAM
TRVINKYIADNEIAKKAKVDTSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRLPVEEVVAKALSDFLLETPVDAK
IICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKGK
ILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVYI
AQPPLYKIKHGKEERYIKDDVEMAAYLVRQALDTAVLVRPDGTEIAGDALAELARQYQLSRGVIERLSRVIDADALRAIA
EGVSLDLSNEAAAEASARTLKARLLEMQGNASSANGGATADAFMQYDEKTEKYRIMVVRRQHGNQRLSHIDADFVAGADY
TALSSTAATFQGLIGDGAKVRRGTGDKVREQNVADFHAAMTWLLSEAERGVSRQRYKGLGEMNPEQLWETTMDVTQRRLL
RVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=566, Percent_Identity=25.2650176678445, Blast_Score=114, Evalue=6e-25,
Organism=Homo sapiens, GI19913406, Length=554, Percent_Identity=24.5487364620939, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI48994957, Length=834, Percent_Identity=59.7122302158273, Blast_Score=960, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=565, Percent_Identity=39.8230088495575, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=608, Percent_Identity=24.6710526315789, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=525, Percent_Identity=24.1904761904762, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=34.1935483870968, Blast_Score=73, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=603, Percent_Identity=23.8805970149254, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17136538, Length=551, Percent_Identity=23.956442831216, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93073; Mature: 92942

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEQKPQSTPAEANSYDAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
DEALAGHCTEIQVTIHTDNSISVIDNGRGIPTGIKFDDKHEPKRSATEIAMTELHAGGKF
HHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
NQNSYKVSGGLHGVGVSCVNGLSKWMKVTVRQGGKVHYIEFAQGVPQNRLIETVQGPDGQ
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCC
MVEVSPLRVLGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIRLTDQ
EEEECCEEEEECCCCCCCEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTIFFAAGEKDGVAVEVSMQWNDAYNEQVLC
CCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEE
FTNNIPQRDGGTHLTGLRAAMTRVINKYIADNEIAKKAKVDTSGDDMREGLTCVLSVKVP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCEEEEEEECC
EPKFSSQTKDKLVSSEVRLPVEEVVAKALSDFLLETPVDAKIICGKIVEAARAREAARKA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
REMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCC
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRT
EEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
LLLTFFYRQMPEIIERGHVYIAQPPLYKIKHGKEERYIKDDVEMAAYLVRQALDTAVLVR
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
PDGTEIAGDALAELARQYQLSRGVIERLSRVIDADALRAIAEGVSLDLSNEAAAEASART
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEECCCHHHHHHHHHH
LKARLLEMQGNASSANGGATADAFMQYDEKTEKYRIMVVRRQHGNQRLSHIDADFVAGAD
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHCCHHHHCCCC
YTALSSTAATFQGLIGDGAKVRRGTGDKVREQNVADFHAAMTWLLSEAERGVSRQRYKGL
CHHHHHHHHHHHHHHCCCCCEECCCCCHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHCCC
GEMNPEQLWETTMDVTQRRLLRVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECC
DV
CC
>Mature Secondary Structure 
TEEQKPQSTPAEANSYDAASIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
DEALAGHCTEIQVTIHTDNSISVIDNGRGIPTGIKFDDKHEPKRSATEIAMTELHAGGKF
HHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
NQNSYKVSGGLHGVGVSCVNGLSKWMKVTVRQGGKVHYIEFAQGVPQNRLIETVQGPDGQ
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCC
MVEVSPLRVLGTTDKRGTEVHFLADEEIFTNVEYHYEILSKRIRELSFLNNGVHIRLTDQ
EEEECCEEEEECCCCCCCEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RTGKEEDFAFSGGVKGFVEYINKNKTVLHPTIFFAAGEKDGVAVEVSMQWNDAYNEQVLC
CCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEE
FTNNIPQRDGGTHLTGLRAAMTRVINKYIADNEIAKKAKVDTSGDDMREGLTCVLSVKVP
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCEEEEEEECC
EPKFSSQTKDKLVSSEVRLPVEEVVAKALSDFLLETPVDAKIICGKIVEAARAREAARKA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
REMTRRKGVLDGMGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKFQAILPLKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCC
KILNVERARFDKMLSSQEVLTLITAMGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRT
EEECHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
LLLTFFYRQMPEIIERGHVYIAQPPLYKIKHGKEERYIKDDVEMAAYLVRQALDTAVLVR
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
PDGTEIAGDALAELARQYQLSRGVIERLSRVIDADALRAIAEGVSLDLSNEAAAEASART
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEECCCHHHHHHHHHH
LKARLLEMQGNASSANGGATADAFMQYDEKTEKYRIMVVRRQHGNQRLSHIDADFVAGAD
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHCCHHHHCCCC
YTALSSTAATFQGLIGDGAKVRRGTGDKVREQNVADFHAAMTWLLSEAERGVSRQRYKGL
CHHHHHHHHHHHHHHCCCCCEECCCCCHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHCCC
GEMNPEQLWETTMDVTQRRLLRVQIEDAIAADQIFTTLMGDDVEPRRNFIESNALVARNI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECC
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]