| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is dnaN [H]
Identifier: 187927114
GI number: 187927114
Start: 2194
End: 3309
Strand: Direct
Name: dnaN [H]
Synonym: Rpic_0002
Alternate gene names: 187927114
Gene position: 2194-3309 (Clockwise)
Preceding gene: 187927113
Following gene: 187927115
Centisome position: 0.06
GC content: 61.47
Gene sequence:
>1116_bases ATGCAATTGGTCAAAACCTCGCGAGACAACCTGCTGCGTCCGCTGCAAATCGTGAGCGGCATCGTGGAACGCCGACACAC GCTGCCGATCCTGGCCAATCTGCTGATCACCAAGCAAGGTGAACGGGTTTCCTTCCTGTCCACCGACATCGAAATCCAGA TCACCACGCACGCCGATTGCGGCGCCGGCGCGGGCGACGTTGCCACCACCGTGGCCGCCCGCAAGCTGGTCGACATCCTG CGTGCCATGCCTGACGGCGAAGTCGCCCTCACGCTCAACGACAAGCGCATGACGGTGCAGTCCGGCAAGAGCCGCTTTGC GCTGCAGACGCTGGCCGCCGAAGAATTCCCGACCGTTGCAGAGGCAACCGATTTCGGTGCCCGCATCACGCTGCCGCAGA AGACGCTCAAGCACTTGCTGGCGATGGTGCACTTCGCGATGGCCCAGCAAGACATCCGCTACTACCTCAACGGCATGCTG CTGGTGGTGGACGGCAAGCAAGTCATGGCCGTCGCCACCGACGGCCACCGCCTGGCCTACTGCGGCGTGGAAACCGGCGA GCAACCGGCAGGAGCCGGCGGCCGCCAGGAAGTGATCATCCCGCGCAAGACCATCCTCGAACTGCAACGCCTGCTGGAAG ACGTGGACGATCCCGTCTCGGTGCAACTGGCATCGAACCAGGTCAAGTTCACTTTCGGCAATATCGAGCTGATCTCCAAG CTGGTCGAAGGCAAGTTCCCGGATTTCCAGCGCGTGATCCCGAAGGGTTACCGCAACAGCTTCACGATCGACCGTGCCTT CCTGCAGCAGGCGCTGCAACGCACGGCCATCCTGACCACCGACAAGTTCAAGGGCGTGCGCTGCATGCTCGACACCAACG TCCTGAAGATCAGCTCCACCAACGCTGACCAGGAAGAAGCGCAGGAAGAACTCGAAATCGACTACCAGGGCGATGCGCTG GATATCGGTTTCAACGTCACCTACCTGCTGGACGTGCTGGCCAACCTCAAGGCCGAAAAAGTGCAGGTGAGCCTGGGCGA CTCCAACTCGAGCGCACTCATCACCCTGCCCGACGACGACACCTTCAAGTACGTCGTCATGCCGATGCGCATTTGA
Upstream 100 bases:
>100_bases AACAACTTTGGGTTAATCGCGAATGCTCCTGCAGGCACCTGGCCATGTGGCCGAGGCTGGGGCGCGCGCGGTGTCACCTC TCAAGCCAAGGATCGCCTTC
Downstream 100 bases:
>100_bases CGCACCGCTGCACCCAGAGCAAGACGAGGGGCTGGATCGTGACGATCCGCCCCTTCGCCGTTTTTAAGCAACCCCGCTGT GTGCGTTGAGATAACCATTT
Product: DNA polymerase III subunit beta
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 371; Mature: 371
Protein sequence:
>371_residues MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLITKQGERVSFLSTDIEIQITTHADCGAGAGDVATTVAARKLVDIL RAMPDGEVALTLNDKRMTVQSGKSRFALQTLAAEEFPTVAEATDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRQEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI
Sequences:
>Translated_371_residues MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLITKQGERVSFLSTDIEIQITTHADCGAGAGDVATTVAARKLVDIL RAMPDGEVALTLNDKRMTVQSGKSRFALQTLAAEEFPTVAEATDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRQEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI >Mature_371_residues MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLITKQGERVSFLSTDIEIQITTHADCGAGAGDVATTVAARKLVDIL RAMPDGEVALTLNDKRMTVQSGKSRFALQTLAAEEFPTVAEATDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGML LVVDGKQVMAVATDGHRLAYCGVETGEQPAGAGGRQEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISSTNADQEEAQEELEIDYQGDAL DIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDDTFKYVVMPMRI
Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped
COG id: COG0592
COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
Organism=Escherichia coli, GI1790136, Length=368, Percent_Identity=44.8369565217391, Blast_Score=309, Evalue=2e-85,
Paralogues:
None
Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001001 - InterPro: IPR022635 - InterPro: IPR022637 - InterPro: IPR022634 [H]
Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 40952; Mature: 40952
Theoretical pI: Translated: 5.29; Mature: 5.29
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLITKQGERVSFLSTDIEIQITTHADC CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCC GAGAGDVATTVAARKLVDILRAMPDGEVALTLNDKRMTVQSGKSRFALQTLAAEEFPTVA CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHCCCHH EATDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKQVMAVATDGHRLAY HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE CGVETGEQPAGAGGRQEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK EECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCHHHHHH LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISST HHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCCEEEEEECCEEEEECC NADQEEAQEELEIDYQGDALDIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDD CCCHHHHHHHCEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCC TFKYVVMPMRI CEEEEEEEECC >Mature Secondary Structure MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLITKQGERVSFLSTDIEIQITTHADC CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCEEEEEEECCCC GAGAGDVATTVAARKLVDILRAMPDGEVALTLNDKRMTVQSGKSRFALQTLAAEEFPTVA CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHCCCHH EATDFGARITLPQKTLKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKQVMAVATDGHRLAY HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE CGVETGEQPAGAGGRQEVIIPRKTILELQRLLEDVDDPVSVQLASNQVKFTFGNIELISK EECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCHHHHHH LVEGKFPDFQRVIPKGYRNSFTIDRAFLQQALQRTAILTTDKFKGVRCMLDTNVLKISST HHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHEEEEECCCCCEEEEEECCEEEEECC NADQEEAQEELEIDYQGDALDIGFNVTYLLDVLANLKAEKVQVSLGDSNSSALITLPDDD CCCHHHHHHHCEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCC TFKYVVMPMRI CEEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12534463 [H]