| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is spoIVB [H]
Identifier: 187935751
GI number: 187935751
Start: 2487207
End: 2488397
Strand: Reverse
Name: spoIVB [H]
Synonym: CLL_A2396
Alternate gene names: 187935751
Gene position: 2488397-2487207 (Counterclockwise)
Preceding gene: 187932364
Following gene: 187934141
Centisome position: 65.48
GC content: 27.46
Gene sequence:
>1191_bases ATGAAGAAGAAAATATTAAAAACTATTAGTTTAATTACAGCTCCAATCTTGATTCTGGTTTTAATAACTAGTATATATTT AAAAGGTATTCCAAATATAATATATACAAATAAAGATATACCAGTATCTTCGATGATCCCTATAGGAAGTACAATAAATA AAATTGAAGATTATGGAAACAAATATCAGGTTAAGTTATTAGGATTAATACCAGTAAAATCATTAGAAGTTCAAAAAGTA AAAAATCTAGAAATTTATCCAGGTGGAAACAGCGTAGGTGTTAGAGTATCAACACAAGGAGTTTTAGTTGTAGGATATTC TGATATAGTAATTAATAATGTAACTGAAGAAAGTCCTGGTAAATTAGGTGGAGTTCAATTAGGAGATATAATATTAAAAA TTAATGGTGAAGAAGTTGAAAATTGCAAAGACTTGGTAGGTATAATTAAAAATTGCAAAGAATGTAAAATAAAAGTTGAC TTACTAAGACACAATGAAAAATTGAATAAAGAAATAGAATTGAAAAAAGAAGAAAATTCAGATTATAAACTAGGATTATG GATTAGAGATTCTACCGCAGGTGTAGGAACAATGACTTTTATAGACAGTGAATCAAAAAAATTTGGTGCATTAGGCCATC CTATTACCGACTGTGATACAAATGAAGCATTTTTAATTAAAAAAGGTGATTTATTAGAATCATCAATAATAAGCTTAAGA AAAGGTGAAAAAGGATCCCCAGGAGAACTTAAAGGTGTATTTATAAATGAAAATAATGTAAGTGGTAAAATAAAAAATAA TACTGAGAGTGGTATTTTTGGAACAGTTGAAAATATGAATATTATAAAGAGTAATGTTAAACCTATGAAAGTTGGTTTTA GAAATGAGGTATGTGAAGGAAAAGCAAGTATAATAACAACTATTGACGAATCTGGACCTAAGGAATATGAAATAGAAATA GTTAAACTTTTGGAACAAAATACTCCTGGACCTAAAAGTATGGTAATAAAAGTTACAGATCCTAAATTATTAGAAAAAAC AGGAGGAATAGTTCAAGGAATGAGTGGAAGTCCTATTATTCAAAATAATAAAATTGTAGGAGCAGTAACTCATGTACTAA TAAATAAGCCAGATACAGGCTATGGAATATATATAGAATGGATGCTAAAAGATGCAGAAATAATAAAATAG
Upstream 100 bases:
>100_bases CATGAACTAAAAATTTTTGACTTTTATAACAATGTTAATACAGCATAAGAGGCTTGTCTCATGGGGAAATTAAAATCAGA AGCAAAGGAGGAGGGAAATT
Downstream 100 bases:
>100_bases TAGTAATATTAAAAGCACTAATTTTTATAATTAGTGCTTTTATAATAAAAAAATAATGAGTTTTACAAACTATTTTCTAC AAAACTATTTACTTATATTA
Product: stage IV sporulation protein B
Products: NA
Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]
Number of amino acids: Translated: 396; Mature: 396
Protein sequence:
>396_residues MKKKILKTISLITAPILILVLITSIYLKGIPNIIYTNKDIPVSSMIPIGSTINKIEDYGNKYQVKLLGLIPVKSLEVQKV KNLEIYPGGNSVGVRVSTQGVLVVGYSDIVINNVTEESPGKLGGVQLGDIILKINGEEVENCKDLVGIIKNCKECKIKVD LLRHNEKLNKEIELKKEENSDYKLGLWIRDSTAGVGTMTFIDSESKKFGALGHPITDCDTNEAFLIKKGDLLESSIISLR KGEKGSPGELKGVFINENNVSGKIKNNTESGIFGTVENMNIIKSNVKPMKVGFRNEVCEGKASIITTIDESGPKEYEIEI VKLLEQNTPGPKSMVIKVTDPKLLEKTGGIVQGMSGSPIIQNNKIVGAVTHVLINKPDTGYGIYIEWMLKDAEIIK
Sequences:
>Translated_396_residues MKKKILKTISLITAPILILVLITSIYLKGIPNIIYTNKDIPVSSMIPIGSTINKIEDYGNKYQVKLLGLIPVKSLEVQKV KNLEIYPGGNSVGVRVSTQGVLVVGYSDIVINNVTEESPGKLGGVQLGDIILKINGEEVENCKDLVGIIKNCKECKIKVD LLRHNEKLNKEIELKKEENSDYKLGLWIRDSTAGVGTMTFIDSESKKFGALGHPITDCDTNEAFLIKKGDLLESSIISLR KGEKGSPGELKGVFINENNVSGKIKNNTESGIFGTVENMNIIKSNVKPMKVGFRNEVCEGKASIITTIDESGPKEYEIEI VKLLEQNTPGPKSMVIKVTDPKLLEKTGGIVQGMSGSPIIQNNKIVGAVTHVLINKPDTGYGIYIEWMLKDAEIIK >Mature_396_residues MKKKILKTISLITAPILILVLITSIYLKGIPNIIYTNKDIPVSSMIPIGSTINKIEDYGNKYQVKLLGLIPVKSLEVQKV KNLEIYPGGNSVGVRVSTQGVLVVGYSDIVINNVTEESPGKLGGVQLGDIILKINGEEVENCKDLVGIIKNCKECKIKVD LLRHNEKLNKEIELKKEENSDYKLGLWIRDSTAGVGTMTFIDSESKKFGALGHPITDCDTNEAFLIKKGDLLESSIISLR KGEKGSPGELKGVFINENNVSGKIKNNTESGIFGTVENMNIIKSNVKPMKVGFRNEVCEGKASIITTIDESGPKEYEIEI VKLLEQNTPGPKSMVIKVTDPKLLEKTGGIVQGMSGSPIIQNNKIVGAVTHVLINKPDTGYGIYIEWMLKDAEIIK
Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c
COG id: COG0750
COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1
Gene ontology:
Cell location: Forespore intermembrane space [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 peptidase S55 domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001478 - InterPro: IPR014219 - InterPro: IPR008763 [H]
Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]
EC number: =3.4.21.116 [H]
Molecular weight: Translated: 43320; Mature: 43320
Theoretical pI: Translated: 8.63; Mature: 8.63
Prosite motif: PS50106 PDZ
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKKILKTISLITAPILILVLITSIYLKGIPNIIYTNKDIPVSSMIPIGSTINKIEDYGN CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCHHHHHHHCCC KYQVKLLGLIPVKSLEVQKVKNLEIYPGGNSVGVRVSTQGVLVVGYSDIVINNVTEESPG EEEEEEEEEECCCCCCCEECCCEEEEECCCEEEEEEECCCEEEEEECCEEEECCCCCCCC KLGGVQLGDIILKINGEEVENCKDLVGIIKNCKECKIKVDLLRHNEKLNKEIELKKEENS CCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCHHCEEEEEEECCCHHCCCEEEEEECCCC DYKLGLWIRDSTAGVGTMTFIDSESKKFGALGHPITDCDTNEAFLIKKGDLLESSIISLR CEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH KGEKGSPGELKGVFINENNVSGKIKNNTESGIFGTVENMNIIKSNVKPMKVGFRNEVCEG CCCCCCCCCEEEEEEECCCCCEEECCCCCCCEEEEECCCEEECCCCCEEEECCCHHHCCC KASIITTIDESGPKEYEIEIVKLLEQNTPGPKSMVIKVTDPKLLEKTGGIVQGMSGSPII CEEEEEEECCCCCCCCHHHEEEEHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCCEE QNNKIVGAVTHVLINKPDTGYGIYIEWMLKDAEIIK ECCEEEEEEEEEEECCCCCCCEEEEEEEECCCHHCC >Mature Secondary Structure MKKKILKTISLITAPILILVLITSIYLKGIPNIIYTNKDIPVSSMIPIGSTINKIEDYGN CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCHHHHHHHCCC KYQVKLLGLIPVKSLEVQKVKNLEIYPGGNSVGVRVSTQGVLVVGYSDIVINNVTEESPG EEEEEEEEEECCCCCCCEECCCEEEEECCCEEEEEEECCCEEEEEECCEEEECCCCCCCC KLGGVQLGDIILKINGEEVENCKDLVGIIKNCKECKIKVDLLRHNEKLNKEIELKKEENS CCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCHHCEEEEEEECCCHHCCCEEEEEECCCC DYKLGLWIRDSTAGVGTMTFIDSESKKFGALGHPITDCDTNEAFLIKKGDLLESSIISLR CEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH KGEKGSPGELKGVFINENNVSGKIKNNTESGIFGTVENMNIIKSNVKPMKVGFRNEVCEG CCCCCCCCCEEEEEEECCCCCEEECCCCCCCEEEEECCCEEECCCCCEEEECCCHHHCCC KASIITTIDESGPKEYEIEIVKLLEQNTPGPKSMVIKVTDPKLLEKTGGIVQGMSGSPII CEEEEEEECCCCCCCCHHHEEEEHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCCEE QNNKIVGAVTHVLINKPDTGYGIYIEWMLKDAEIIK ECCEEEEEEEEEEECCCCCCCEEEEEEEECCCHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 2106508; 8969508; 9384377; 11741860 [H]