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The map label for this gene is spo0A [H]

Identifier: 187934141

GI number: 187934141

Start: 2486005

End: 2486823

Strand: Reverse

Name: spo0A [H]

Synonym: CLL_A2395

Alternate gene names: 187934141

Gene position: 2486823-2486005 (Counterclockwise)

Preceding gene: 187935751

Following gene: 187935337

Centisome position: 65.44

GC content: 29.18

Gene sequence:

>819_bases
ATGGAAGATAAAAAAATATCTGTACTTATTGCTGATGATAATAAGGAATTTTGCAGTATTTTAAACGATTATTTATTAAA
TCAAAGAAATATAATAGTAACAGGAATAGCAAAAGATGGTAGGGAAGCTTTAGATTTAGTGGTTGAAAAGAAACCAGATT
TATTAATTCTAGATATAATAATGCCACATTTAGACGGTTTAGGTGTTTTAGAAAGATTAAATACTATGAATTTAGAAAAA
ATGCCTAAGATTATAATCTTATCTGCAGTAGGTCAAGATAAAATTACACAACAGGCAATAACATTAGGAGCAGACTATTA
TACTGTTAAGCCATTTGATATGGAAGTATTTACAAAGAGAATAAGAGAAATGTTCAATAGTGAAACAGACAACCAAAGTA
TAAGAAGAACATCATCACAACCTATGATTTCTTATACTAGTGAATGTCAAGATAATAAAGGAAGTTTAGATTTAGAAACA
GAAATAACAAATATTATACATGAAATTGGTGTACCGGCTCATATAAAGGGATATATGTATTTAAGGGAAGCAATAACTAT
GGTTGTTAATGATATAGAATTATTATCAGCTGTAACAAAGGAATTATATCCATCAATAGCTAAGAAATATAACACAACAG
CTTCAAGAGTGGAAAGAGCAATAAGACATGCTATAGAAGTTGCTTGGGGCAGAGGCCAAATAGAAGCTATAAACAAGCTA
TTTGGATATACTATACACAATGATAAGGGCAAGCCTACTAATAGCGAATTTATAGCAATTATTGCAGATAAGCTAAGACT
TAAAAATAAAGTTAGCTAA

Upstream 100 bases:

>100_bases
TATTTAGTGATAAGTTTAGCTATTAAAAGTAGTTTATGATGTTATAGAGATTGTGATTCAAATAGTAACATAATTAGGTT
TATGAAGGAGAGGAAAAAGC

Downstream 100 bases:

>100_bases
AAGGCTTGGTTATGCGATTCTTGTGAATGGCGATAGCGATAAACTCTATAAAAATCGCTATGGATTTTGATGTTTTATGT
CTATAAATATAATATTAATT

Product: sporulation transcription factor Spo0A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MEDKKISVLIADDNKEFCSILNDYLLNQRNIIVTGIAKDGREALDLVVEKKPDLLILDIIMPHLDGLGVLERLNTMNLEK
MPKIIILSAVGQDKITQQAITLGADYYTVKPFDMEVFTKRIREMFNSETDNQSIRRTSSQPMISYTSECQDNKGSLDLET
EITNIIHEIGVPAHIKGYMYLREAITMVVNDIELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWGRGQIEAINKL
FGYTIHNDKGKPTNSEFIAIIADKLRLKNKVS

Sequences:

>Translated_272_residues
MEDKKISVLIADDNKEFCSILNDYLLNQRNIIVTGIAKDGREALDLVVEKKPDLLILDIIMPHLDGLGVLERLNTMNLEK
MPKIIILSAVGQDKITQQAITLGADYYTVKPFDMEVFTKRIREMFNSETDNQSIRRTSSQPMISYTSECQDNKGSLDLET
EITNIIHEIGVPAHIKGYMYLREAITMVVNDIELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWGRGQIEAINKL
FGYTIHNDKGKPTNSEFIAIIADKLRLKNKVS
>Mature_272_residues
MEDKKISVLIADDNKEFCSILNDYLLNQRNIIVTGIAKDGREALDLVVEKKPDLLILDIIMPHLDGLGVLERLNTMNLEK
MPKIIILSAVGQDKITQQAITLGADYYTVKPFDMEVFTKRIREMFNSETDNQSIRRTSSQPMISYTSECQDNKGSLDLET
EITNIIHEIGVPAHIKGYMYLREAITMVVNDIELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWGRGQIEAINKL
FGYTIHNDKGKPTNSEFIAIIADKLRLKNKVS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 30786; Mature: 30786

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKKISVLIADDNKEFCSILNDYLLNQRNIIVTGIAKDGREALDLVVEKKPDLLILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEHEEH
MPHLDGLGVLERLNTMNLEKMPKIIILSAVGQDKITQQAITLGADYYTVKPFDMEVFTKR
HHCCCCHHHHHHHHHCCHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
IREMFNSETDNQSIRRTSSQPMISYTSECQDNKGSLDLETEITNIIHEIGVPAHIKGYMY
HHHHHCCCCCCHHHHHCCCCCCEEEHHHCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHH
LREAITMVVNDIELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWGRGQIEAINKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FGYTIHNDKGKPTNSEFIAIIADKLRLKNKVS
HCEEEECCCCCCCCCCEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MEDKKISVLIADDNKEFCSILNDYLLNQRNIIVTGIAKDGREALDLVVEKKPDLLILDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEHEEH
MPHLDGLGVLERLNTMNLEKMPKIIILSAVGQDKITQQAITLGADYYTVKPFDMEVFTKR
HHCCCCHHHHHHHHHCCHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
IREMFNSETDNQSIRRTSSQPMISYTSECQDNKGSLDLETEITNIIHEIGVPAHIKGYMY
HHHHHCCCCCCHHHHHCCCCCCEEEHHHCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHH
LREAITMVVNDIELLSAVTKELYPSIAKKYNTTASRVERAIRHAIEVAWGRGQIEAINKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FGYTIHNDKGKPTNSEFIAIIADKLRLKNKVS
HCEEEECCCCCCCCCCEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7885226 [H]