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The map label for this gene is recN [H]

Identifier: 187932364

GI number: 187932364

Start: 2488690

End: 2490378

Strand: Reverse

Name: recN [H]

Synonym: CLL_A2397

Alternate gene names: 187932364

Gene position: 2490378-2488690 (Counterclockwise)

Preceding gene: 187932857

Following gene: 187935751

Centisome position: 65.53

GC content: 24.81

Gene sequence:

>1689_bases
ATGTTAATTCAATTGAACATTAAAAATTTTGCATTGATACAAGAAATAACTATGAATTTCAATGAAGGATTTAATATTCT
TTCAGGTGAAACAGGAGCTGGTAAATCTATTTTAATTGATGCGATAGATTATGTATTAGGTGGAAAATTTTCAAAAAGTT
TAATAAGAACTGGAGAAGATAAAACTTATGTTGAGGCTATATTTACTATTGAAAATAGCTTGTTAAAAAATGTATTAATT
GAATTAGATATTGAAAGTGACGATGACATGCTAATTGTCTCTAGAGAAACTCATCAAAGTGGAAGAAGTTTAATTAAAGT
TAATGGAAAAACTTTTTTAGCATCTCAATTAAAAAAAATAAGAGAGAGATTATTAGATATACATGGTCAACATCAAAATC
AAACTCTTCTTCAAAGAAATAGTCATATACTATATTTAGATGAATTTATAGGAAAAGAAATTCTTAATCATTTAGAAGAA
TTCACTTCTTTAAAGGATAGTTTATTACAAATCGAAAACAAAATTCAACAAATATGTGGGAATGATGATAGAGATAAACT
TTTAGACTATCTTAAATTTCAAATTGAGGATATAGAAAAAGGGAAATTAAAAGAAAAAGAAGAAGAAAATTTAAAAGAAG
AATTTAATTTATTATCAAATGCTGAAAAAATAAGCACTAGTTTAAATTTATCATATGCTTTATTAAATGGTGTTGATGGA
GAAAATAGTGTCATAAATTCAATATCAAAGGTTATTCAAGAATTATCGAATATTGAAAATAATTTTGAAAAAGCTAAAAA
AAATAGACAGCTAATAGAAGAAGCTTTTTATACAATAGAAGAAGTGTCTAGAGATATAAGAAATATAGCAGAAGAAGTTG
TATATGATGATAACGCTTTAGAAAAAATAAATGCAAGGATATATGAAATTAATCAATATAAGAAAAAATATGGTGCTACT
ATAACGGAAGTTTTAGAGTACTATGATAAAATAAAAATACAATATAATGATCTTATAAATTCTGAAAAAATTATTGAAGA
ATTAAACTTGAAAAAGCAAGAAATTATAAAAGAAATGGAAATAGTATCACTAAAACTTCATGAATTAAGAGTCAATAAAA
GCACTGAATTAAAAGAAAATATTTTAAATGAACTTAGTTTTGTAGGACTTGAAAAATCAACTATGGAAATAAGTATAGTG
AGAGAAGAAAAATTTAATTCTAGAGGCTTTGATAATGTATGTTTTATGATTTCTACAAATCCTGGAGAACCGCTTATGCC
ATTAGAAAAAATATTATCTGGTGGAGAACTTTCAAGAATAATGCTTGCATTAAAATGCGTTTTTGCAGATAAAGATGAAA
TAGCAACTTTAATTTTTGATGAAATAGATACTGGTATTAGTGGTGTTGTGGCAAAAAGAGTTGGGGAAAAAATGTATCAA
GTATCTAAAAATCACCAAGTTTTATGTATAACACATCTTCCTCAAATAGCAATTTTATCAGATTATCATTACTTTTTATC
AAAGATAGTAAAAGAAAATAAGACATTTACTAATATAAGAATGTTAACCAAAGAAGAAAAAGAGTATCAAATTGGTAGAA
TGCTTGGTGGAGATGAAGTTACAGAAGCAACGTTAAATAATGTGAAAGAAATGATAAAAATTGCCGAGTTAAAAAAAATA
GAAATTTAA

Upstream 100 bases:

>100_bases
TAATACAATATTCATACTTCTTAGAAGTTTAGAAAAAGCAGAAGAATTAGTAGCTAAAATAAGAAAAAGAATATCACTAT
AAATATAAGGATGGGTATAT

Downstream 100 bases:

>100_bases
AAATTAATACATATAGTAATATATTTTGGTAAAAATAAAATCATGTAATACGTGGTTTTATTTTTTTTTATATTATTCCA
AATTTCTTGCAGACGAGTTC

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MLIQLNIKNFALIQEITMNFNEGFNILSGETGAGKSILIDAIDYVLGGKFSKSLIRTGEDKTYVEAIFTIENSLLKNVLI
ELDIESDDDMLIVSRETHQSGRSLIKVNGKTFLASQLKKIRERLLDIHGQHQNQTLLQRNSHILYLDEFIGKEILNHLEE
FTSLKDSLLQIENKIQQICGNDDRDKLLDYLKFQIEDIEKGKLKEKEEENLKEEFNLLSNAEKISTSLNLSYALLNGVDG
ENSVINSISKVIQELSNIENNFEKAKKNRQLIEEAFYTIEEVSRDIRNIAEEVVYDDNALEKINARIYEINQYKKKYGAT
ITEVLEYYDKIKIQYNDLINSEKIIEELNLKKQEIIKEMEIVSLKLHELRVNKSTELKENILNELSFVGLEKSTMEISIV
REEKFNSRGFDNVCFMISTNPGEPLMPLEKILSGGELSRIMLALKCVFADKDEIATLIFDEIDTGISGVVAKRVGEKMYQ
VSKNHQVLCITHLPQIAILSDYHYFLSKIVKENKTFTNIRMLTKEEKEYQIGRMLGGDEVTEATLNNVKEMIKIAELKKI
EI

Sequences:

>Translated_562_residues
MLIQLNIKNFALIQEITMNFNEGFNILSGETGAGKSILIDAIDYVLGGKFSKSLIRTGEDKTYVEAIFTIENSLLKNVLI
ELDIESDDDMLIVSRETHQSGRSLIKVNGKTFLASQLKKIRERLLDIHGQHQNQTLLQRNSHILYLDEFIGKEILNHLEE
FTSLKDSLLQIENKIQQICGNDDRDKLLDYLKFQIEDIEKGKLKEKEEENLKEEFNLLSNAEKISTSLNLSYALLNGVDG
ENSVINSISKVIQELSNIENNFEKAKKNRQLIEEAFYTIEEVSRDIRNIAEEVVYDDNALEKINARIYEINQYKKKYGAT
ITEVLEYYDKIKIQYNDLINSEKIIEELNLKKQEIIKEMEIVSLKLHELRVNKSTELKENILNELSFVGLEKSTMEISIV
REEKFNSRGFDNVCFMISTNPGEPLMPLEKILSGGELSRIMLALKCVFADKDEIATLIFDEIDTGISGVVAKRVGEKMYQ
VSKNHQVLCITHLPQIAILSDYHYFLSKIVKENKTFTNIRMLTKEEKEYQIGRMLGGDEVTEATLNNVKEMIKIAELKKI
EI
>Mature_562_residues
MLIQLNIKNFALIQEITMNFNEGFNILSGETGAGKSILIDAIDYVLGGKFSKSLIRTGEDKTYVEAIFTIENSLLKNVLI
ELDIESDDDMLIVSRETHQSGRSLIKVNGKTFLASQLKKIRERLLDIHGQHQNQTLLQRNSHILYLDEFIGKEILNHLEE
FTSLKDSLLQIENKIQQICGNDDRDKLLDYLKFQIEDIEKGKLKEKEEENLKEEFNLLSNAEKISTSLNLSYALLNGVDG
ENSVINSISKVIQELSNIENNFEKAKKNRQLIEEAFYTIEEVSRDIRNIAEEVVYDDNALEKINARIYEINQYKKKYGAT
ITEVLEYYDKIKIQYNDLINSEKIIEELNLKKQEIIKEMEIVSLKLHELRVNKSTELKENILNELSFVGLEKSTMEISIV
REEKFNSRGFDNVCFMISTNPGEPLMPLEKILSGGELSRIMLALKCVFADKDEIATLIFDEIDTGISGVVAKRVGEKMYQ
VSKNHQVLCITHLPQIAILSDYHYFLSKIVKENKTFTNIRMLTKEEKEYQIGRMLGGDEVTEATLNNVKEMIKIAELKKI
EI

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=563, Percent_Identity=30.7282415630551, Blast_Score=273, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64601; Mature: 64601

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIQLNIKNFALIQEITMNFNEGFNILSGETGAGKSILIDAIDYVLGGKFSKSLIRTGED
CEEEEECCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCC
KTYVEAIFTIENSLLKNVLIELDIESDDDMLIVSRETHQSGRSLIKVNGKTFLASQLKKI
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHH
RERLLDIHGQHQNQTLLQRNSHILYLDEFIGKEILNHLEEFTSLKDSLLQIENKIQQICG
HHHHHHHCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDDRDKLLDYLKFQIEDIEKGKLKEKEEENLKEEFNLLSNAEKISTSLNLSYALLNGVDG
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHCCCCC
ENSVINSISKVIQELSNIENNFEKAKKNRQLIEEAFYTIEEVSRDIRNIAEEVVYDDNAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EKINARIYEINQYKKKYGATITEVLEYYDKIKIQYNDLINSEKIIEELNLKKQEIIKEME
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEHHHHCCHHHHHHHHCCCHHHHHHHHH
IVSLKLHELRVNKSTELKENILNELSFVGLEKSTMEISIVREEKFNSRGFDNVCFMISTN
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCEEEEEECC
PGEPLMPLEKILSGGELSRIMLALKCVFADKDEIATLIFDEIDTGISGVVAKRVGEKMYQ
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSKNHQVLCITHLPQIAILSDYHYFLSKIVKENKTFTNIRMLTKEEKEYQIGRMLGGDEV
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
TEATLNNVKEMIKIAELKKIEI
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIQLNIKNFALIQEITMNFNEGFNILSGETGAGKSILIDAIDYVLGGKFSKSLIRTGED
CEEEEECCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCC
KTYVEAIFTIENSLLKNVLIELDIESDDDMLIVSRETHQSGRSLIKVNGKTFLASQLKKI
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHH
RERLLDIHGQHQNQTLLQRNSHILYLDEFIGKEILNHLEEFTSLKDSLLQIENKIQQICG
HHHHHHHCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NDDRDKLLDYLKFQIEDIEKGKLKEKEEENLKEEFNLLSNAEKISTSLNLSYALLNGVDG
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHCCCCC
ENSVINSISKVIQELSNIENNFEKAKKNRQLIEEAFYTIEEVSRDIRNIAEEVVYDDNAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EKINARIYEINQYKKKYGATITEVLEYYDKIKIQYNDLINSEKIIEELNLKKQEIIKEME
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEHHHHCCHHHHHHHHCCCHHHHHHHHH
IVSLKLHELRVNKSTELKENILNELSFVGLEKSTMEISIVREEKFNSRGFDNVCFMISTN
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCEEEEEECC
PGEPLMPLEKILSGGELSRIMLALKCVFADKDEIATLIFDEIDTGISGVVAKRVGEKMYQ
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSKNHQVLCITHLPQIAILSDYHYFLSKIVKENKTFTNIRMLTKEEKEYQIGRMLGGDEV
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
TEATLNNVKEMIKIAELKKIEI
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]