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The map label for this gene is vanS [H]

Identifier: 187934998

GI number: 187934998

Start: 3178844

End: 3180367

Strand: Reverse

Name: vanS [H]

Synonym: CLL_A3073

Alternate gene names: 187934998

Gene position: 3180367-3178844 (Counterclockwise)

Preceding gene: 187932476

Following gene: 187934395

Centisome position: 83.69

GC content: 24.02

Gene sequence:

>1524_bases
TTGAAAAATAAAGAAAAATTAAAAAACCTTTATGAACAATATAATGAAAAATTAGTTGCTTTAAAGGTTTATAAGTTTTT
TCGTAGCGTATGTTTAAAATTAAGAATAAATATTGAGAGAAGCATTAGATTTGAATTAATGCTAGTAATTGGAGTATGCT
TTTTATTATCTTTTACATTTTATAATTTCACAGATGTACTGTTGAAAAAAGAATATCGAAATCCAGAAGTATTTTATGAT
TATGAATCTACAGAAAAAGAAGCTAATATGTTATTAAAAGAGTTAGAAAGAACGGAACATTTAAAAATAGATGACTCTAA
AGAGATAGAAAAAATATTAAATTCACCTATGTATAATAACAGTAAGATATATATTACAAATACTGAAGGAAAAGTTGTTT
TTAAAACTTCAAATGTTTATGAAGAAACAATAGATATATATAGTGTATTTAGAGATATTATTTCTAATGAAAATGATACT
AGTAATGCAGCACGGGAAAGAATTTATATTATGCCATTAAAAATGGATAATACCAGATTATATTTAATATATTCTAAAAT
TCCAAAGGCAGAAATAGTATATAATACGGTAGAAATGTCTAATTCCTCTTTAGCATTATCACTTACATTTATAATTTTTA
TAATTTCATTTGTAATAATTACTAATAATAAAATGAAGTATTTAGATGAAATTGCATCTGGATTAAAAACAATAGCAAAT
GGAAATTTAAATTATAGGATTGAGGAAAAAGGAAGAGATGAAATTAGAAAGATAGCTAATAACATAAATAATATGGCTAA
GGAGATTGGTGAGAAAATACAAGCTGAAAGAGATGCAGAAAAAACAAAAACTGACTTAATTACAAATGTATCTCATGATT
TGAGAACTCCGTTAACGTCTATTATGGGTTATATAGGTTTGGTAAAAGAAGATAGATACAAAAACAAGGAAGAAATGAAC
GAATATTTAAATATAGCTTATAACAAGGCACAAAAATTAAATATATTAATTGAAGATTTATTTGAATATACGAAGCTTAA
TAATAATGGAATGAAGTTAACTAAAGAAAAAGTTAATTTATGTGAACTTCTATCTCAATTAATAGAAGAGATGACGCCAA
TCGTAGATGAAAATTCAGTAAGTATAAATAAAAAATTTGATATAGATAGAGCATATGTTTTTATAGATATAGTAAAAATG
CTTAGAGTGTTTGAAAATTTAATAATGAATGCTGTAAAGTATTCAATTAAACCTGGAGAAATAATCATAGGTCTTTACCA
AAAAGATGATTATGTAACTTTTGTGTGTAGAAATAAGGGAGAATATATATCTAAAGAAAAATTAGATAAAGTATTTGATC
GATTTTATAGAGTTGATGAATCAAGAAATACTACTACAGGGGGATCAGGATTAGGCCTCGCTATTTCTAAAAATATAATT
AATTTACATGAAGGTAAAATATGGGCTGAATGTATTGATGATAATATAAGTTTTTATGTTAATTTAAAGATAGAAGATTT
ATAG

Upstream 100 bases:

>100_bases
AATACTGTAATGGTACATATAAGAAAAATAAGAGAAAAAATAGAAAGTGATCCAAGAACCCCTAAATTTATAAAGACCGT
ATGGGGAGTTGGGTATAAAA

Downstream 100 bases:

>100_bases
TTATTATATAATTATATTTTATTGGTACTTTTTTTGAGTAACTTTAATATTTTATAATAAGACTACTTTTTATTTGGAGG
AAATATGCAAGATAAAAATC

Product: two-component sensor kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKNKEKLKNLYEQYNEKLVALKVYKFFRSVCLKLRINIERSIRFELMLVIGVCFLLSFTFYNFTDVLLKKEYRNPEVFYD
YESTEKEANMLLKELERTEHLKIDDSKEIEKILNSPMYNNSKIYITNTEGKVVFKTSNVYEETIDIYSVFRDIISNENDT
SNAARERIYIMPLKMDNTRLYLIYSKIPKAEIVYNTVEMSNSSLALSLTFIIFIISFVIITNNKMKYLDEIASGLKTIAN
GNLNYRIEEKGRDEIRKIANNINNMAKEIGEKIQAERDAEKTKTDLITNVSHDLRTPLTSIMGYIGLVKEDRYKNKEEMN
EYLNIAYNKAQKLNILIEDLFEYTKLNNNGMKLTKEKVNLCELLSQLIEEMTPIVDENSVSINKKFDIDRAYVFIDIVKM
LRVFENLIMNAVKYSIKPGEIIIGLYQKDDYVTFVCRNKGEYISKEKLDKVFDRFYRVDESRNTTTGGSGLGLAISKNII
NLHEGKIWAECIDDNISFYVNLKIEDL

Sequences:

>Translated_507_residues
MKNKEKLKNLYEQYNEKLVALKVYKFFRSVCLKLRINIERSIRFELMLVIGVCFLLSFTFYNFTDVLLKKEYRNPEVFYD
YESTEKEANMLLKELERTEHLKIDDSKEIEKILNSPMYNNSKIYITNTEGKVVFKTSNVYEETIDIYSVFRDIISNENDT
SNAARERIYIMPLKMDNTRLYLIYSKIPKAEIVYNTVEMSNSSLALSLTFIIFIISFVIITNNKMKYLDEIASGLKTIAN
GNLNYRIEEKGRDEIRKIANNINNMAKEIGEKIQAERDAEKTKTDLITNVSHDLRTPLTSIMGYIGLVKEDRYKNKEEMN
EYLNIAYNKAQKLNILIEDLFEYTKLNNNGMKLTKEKVNLCELLSQLIEEMTPIVDENSVSINKKFDIDRAYVFIDIVKM
LRVFENLIMNAVKYSIKPGEIIIGLYQKDDYVTFVCRNKGEYISKEKLDKVFDRFYRVDESRNTTTGGSGLGLAISKNII
NLHEGKIWAECIDDNISFYVNLKIEDL
>Mature_507_residues
MKNKEKLKNLYEQYNEKLVALKVYKFFRSVCLKLRINIERSIRFELMLVIGVCFLLSFTFYNFTDVLLKKEYRNPEVFYD
YESTEKEANMLLKELERTEHLKIDDSKEIEKILNSPMYNNSKIYITNTEGKVVFKTSNVYEETIDIYSVFRDIISNENDT
SNAARERIYIMPLKMDNTRLYLIYSKIPKAEIVYNTVEMSNSSLALSLTFIIFIISFVIITNNKMKYLDEIASGLKTIAN
GNLNYRIEEKGRDEIRKIANNINNMAKEIGEKIQAERDAEKTKTDLITNVSHDLRTPLTSIMGYIGLVKEDRYKNKEEMN
EYLNIAYNKAQKLNILIEDLFEYTKLNNNGMKLTKEKVNLCELLSQLIEEMTPIVDENSVSINKKFDIDRAYVFIDIVKM
LRVFENLIMNAVKYSIKPGEIIIGLYQKDDYVTFVCRNKGEYISKEKLDKVFDRFYRVDESRNTTTGGSGLGLAISKNII
NLHEGKIWAECIDDNISFYVNLKIEDL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=29.0322580645161, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786783, Length=448, Percent_Identity=21.2053571428571, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787894, Length=315, Percent_Identity=26.3492063492064, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=28.9719626168224, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790346, Length=254, Percent_Identity=27.5590551181102, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=26.0683760683761, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788713, Length=261, Percent_Identity=25.6704980842912, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=25.7510729613734, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=260, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=472, Percent_Identity=20.7627118644068, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1789808, Length=307, Percent_Identity=26.0586319218241, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=28.7671232876712, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082128, Length=288, Percent_Identity=21.875, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789149, Length=352, Percent_Identity=22.1590909090909, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790436, Length=207, Percent_Identity=27.0531400966184, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788279, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787374, Length=321, Percent_Identity=21.8068535825545, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790551, Length=238, Percent_Identity=23.9495798319328, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59315; Mature: 59315

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKEKLKNLYEQYNEKLVALKVYKFFRSVCLKLRINIERSIRFELMLVIGVCFLLSFTF
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
YNFTDVLLKKEYRNPEVFYDYESTEKEANMLLKELERTEHLKIDDSKEIEKILNSPMYNN
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
SKIYITNTEGKVVFKTSNVYEETIDIYSVFRDIISNENDTSNAARERIYIMPLKMDNTRL
CEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEEEEECCCEE
YLIYSKIPKAEIVYNTVEMSNSSLALSLTFIIFIISFVIITNNKMKYLDEIASGLKTIAN
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GNLNYRIEEKGRDEIRKIANNINNMAKEIGEKIQAERDAEKTKTDLITNVSHDLRTPLTS
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IMGYIGLVKEDRYKNKEEMNEYLNIAYNKAQKLNILIEDLFEYTKLNNNGMKLTKEKVNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
CELLSQLIEEMTPIVDENSVSINKKFDIDRAYVFIDIVKMLRVFENLIMNAVKYSIKPGE
HHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIIGLYQKDDYVTFVCRNKGEYISKEKLDKVFDRFYRVDESRNTTTGGSGLGLAISKNII
EEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEE
NLHEGKIWAECIDDNISFYVNLKIEDL
ECCCCCEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MKNKEKLKNLYEQYNEKLVALKVYKFFRSVCLKLRINIERSIRFELMLVIGVCFLLSFTF
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
YNFTDVLLKKEYRNPEVFYDYESTEKEANMLLKELERTEHLKIDDSKEIEKILNSPMYNN
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCC
SKIYITNTEGKVVFKTSNVYEETIDIYSVFRDIISNENDTSNAARERIYIMPLKMDNTRL
CEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEEEEECCCEE
YLIYSKIPKAEIVYNTVEMSNSSLALSLTFIIFIISFVIITNNKMKYLDEIASGLKTIAN
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GNLNYRIEEKGRDEIRKIANNINNMAKEIGEKIQAERDAEKTKTDLITNVSHDLRTPLTS
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IMGYIGLVKEDRYKNKEEMNEYLNIAYNKAQKLNILIEDLFEYTKLNNNGMKLTKEKVNL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
CELLSQLIEEMTPIVDENSVSINKKFDIDRAYVFIDIVKMLRVFENLIMNAVKYSIKPGE
HHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIIGLYQKDDYVTFVCRNKGEYISKEKLDKVFDRFYRVDESRNTTTGGSGLGLAISKNII
EEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEE
NLHEGKIWAECIDDNISFYVNLKIEDL
ECCCCCEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]